{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T04:24:02Z","timestamp":1793075042567,"version":"4.5.0"},"reference-count":126,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T00:00:00Z","timestamp":1734393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI167692"],"award-info":[{"award-number":["R01AI167692"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R24GM137786"],"award-info":[{"award-number":["R24GM137786"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p><jats:italic>Cryptococcus neoformans<\/jats:italic> is a pathogenic basidiomycetous yeast that primarily infects immunocompromised individuals. Fatal outcome of cryptococcosis depends on the ability of <jats:italic>C<\/jats:italic>. <jats:italic>neoformans<\/jats:italic> to sense and adapt to 37\u00b0C. A complex of conserved filament forming GTPases, called septins, composed of Cdc3, Cdc10, Cdc11, and Cdc12, assembles at the mother-bud neck in <jats:italic>C<\/jats:italic>. <jats:italic>neoformans<\/jats:italic>. Septins Cdc3 and Cdc12 are essential for proliferation of <jats:italic>C<\/jats:italic>. <jats:italic>neoformans<\/jats:italic> at 37\u00b0C and for virulence in the <jats:italic>Galleria mellonella<\/jats:italic> model of infection, presumably due to their requirement for septin complex formation, and the involvement in cytokinesis. However, how exactly Cdc3, and Cdc12 contribute to <jats:italic>C<\/jats:italic>. <jats:italic>neoformans<\/jats:italic> growth at 37\u00b0C remains unknown. Based on studies investigating roles of septins in <jats:italic>Saccharomyces cerevisiae<\/jats:italic>, septin complex at the mother-bud neck of <jats:italic>C<\/jats:italic>. <jats:italic>neoformans<\/jats:italic> is predicted to interact with proteins involved in cell cycle control, morphogenesis, and cytokinesis, but the septin-associated proteome in <jats:italic>C<\/jats:italic>. <jats:italic>neoformans<\/jats:italic> has not been investigated. Here, we utilized tandem mass spectrometry to define <jats:italic>C<\/jats:italic>. <jats:italic>neoformans<\/jats:italic> proteins that associate with either Cdc3 or Cdc10 at \u223c25\u00b0C or after the shift to 37\u00b0C. Our findings unveil a diverse array of septin-associated proteins, highlighting potential roles of septins in cell division, and stress response. Two proteins, identified as associated with both Cdc3 and Cdc10, the actin-binding protein profilin, which was detected at both temperatures, and ATP-binding multi-drug transporter Afr1, which was detected exclusively at 37\u00b0C, were further confirmed by co-immunoprecipitation. We also confirmed that association of Cdc3 with Afr1 was enhanced at 37\u00b0C. Upon shift to 37\u00b0C, septins Cdc3 and Cdc10 exhibited altered localization and Cdc3 partially co-localized with Afr1. In addition, we also investigated changes to levels of individual <jats:italic>C<\/jats:italic>. <jats:italic>neoformans<\/jats:italic> proteins upon shift from \u223c25 to 37\u00b0C in exponentially grown culture and when cells entered stationary phase at \u223c25\u00b0C. Our study reveals changes to <jats:italic>C<\/jats:italic>. <jats:italic>neoformans<\/jats:italic> proteome associated with heat and nutrient deprivation stresses and provides a landscape of septin-associated <jats:italic>C<\/jats:italic>. <jats:italic>neoformans<\/jats:italic> proteome, which will facilitate elucidating the biology of septins and mechanisms of stress response in this fungal pathogen.<\/jats:p>","DOI":"10.1371\/journal.pone.0313444","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T18:23:36Z","timestamp":1734459816000},"page":"e0313444","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Quantitative analysis of septin Cdc10 &amp; Cdc3-associated proteome during stress response in the fungal pathogen Cryptococcus neoformans"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9350-3576","authenticated-orcid":true,"given":"Stephani","family":"Martinez Barrera","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9343-6534","authenticated-orcid":true,"given":"Emma","family":"Hatchell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1783-3610","authenticated-orcid":true,"given":"Stephanie D.","family":"Byrum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel G.","family":"Mackintosh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9560-782X","authenticated-orcid":true,"given":"Lukasz","family":"Kozubowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,12,17]]},"reference":[{"issue":"12","key":"pone.0313444.ref001","doi-asserted-by":"crossref","first-page":"1748","DOI":"10.1016\/S1473-3099(22)00499-6","article-title":"The global burden of HIV-associated cryptococcal infection in adults in 2020: a modelling analysis","volume":"22","author":"R Rajasingham","year":"2022","journal-title":"Lancet Infect Dis"},{"issue":"1","key":"pone.0313444.ref002","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s40249-023-01073-4","article-title":"Cryptococcus neoformans, a global threat to human health","volume":"12","author":"Y Zhao","year":"2023","journal-title":"Infect Dis Poverty"},{"issue":"10","key":"pone.0313444.ref003","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1038\/nrmicro1245","article-title":"Deciphering the model pathogenic fungus Cryptococcus neoformans","volume":"3","author":"A Idnurm","year":"2005","journal-title":"Nat Rev Microbiol"},{"key":"pone.0313444.ref004","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/srep21489","article-title":"Cryptococcus neoformans Intracellular Proliferation and Capsule Size Determines Early Macrophage Control of Infection","volume":"6","author":"A Bojarczuk","year":"2016","journal-title":"Sci Rep"},{"issue":"4","key":"pone.0313444.ref005","doi-asserted-by":"crossref","first-page":"2012","DOI":"10.1128\/IAI.73.4.2012-2019.2005","article-title":"Melanization of Cryptococcus neoformans affects lung inflammatory responses during cryptococcal infection","volume":"73","author":"AJ Mednick","year":"2005","journal-title":"Infect Immun"},{"issue":"3","key":"pone.0313444.ref006","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1111\/cmi.12067","article-title":"Cryptococcus interactions with macrophages: Evasion and manipulation of the phagosome by a fungal pathogen","volume":"15","author":"SA Johnston","year":"2013","journal-title":"Cell Microbiol"},{"issue":"21","key":"pone.0313444.ref007","doi-asserted-by":"crossref","first-page":"2156","DOI":"10.1016\/j.cub.2006.09.032","article-title":"Expulsion of Live Pathogenic Yeast by Macrophages","volume":"16","author":"H Ma","year":"2006","journal-title":"Current Biology"},{"issue":"3","key":"pone.0313444.ref008","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1111\/j.1365-2958.2009.06983.x","article-title":"Septins enforce morphogenetic events during sexual reproduction and contribute to virulence of Cryptococcus neoformans","volume":"75","author":"L Kozubowski","year":"2010","journal-title":"Mol Microbiol"},{"key":"pone.0313444.ref009","first-page":"289","volume-title":"International Review of Cell and Molecular Biology","author":"Elsevier Inc","year":"2014","edition":"1"},{"key":"pone.0313444.ref010","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2148-7-1","article-title":"Analysis of septins across kingdoms reveals orthology and new motifs","volume":"7","author":"F Pan","year":"2007","journal-title":"BMC Evol Biol"},{"issue":"6","key":"pone.0313444.ref011","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1038\/nrm2407","article-title":"The septin family of GTPases: Architecture and dynamics","volume":"9","author":"CS Weirich","year":"2008","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"3","key":"pone.0313444.ref012","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1038\/nrm3284","article-title":"Septins: the fourth component of the cytoskeleton","volume":"13","author":"S Mostowy","year":"2012","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"9","key":"pone.0313444.ref013","first-page":"1503","article-title":"Septin Function in Yeast Model Systems and Pathogenic Fungi MINIREVIEW Septin Function in Yeast Model Systems and Pathogenic Fungi","volume":"4","author":"LM Douglas","year":"2005","journal-title":"Society"},{"issue":"6","key":"pone.0313444.ref014","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1016\/S1369-5274(01)00269-7","article-title":"The septin cortex at the yeast mother-bud neck","volume":"4","author":"AS Gladfelter","year":"2001","journal-title":"Curr Opin Microbiol"},{"issue":"4","key":"pone.0313444.ref015","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1016\/j.devcel.2011.02.004","article-title":"Septin Filament Formation Is Essential in Budding Yeast","volume":"20","author":"MA McMurray","year":"2011","journal-title":"Dev Cell"},{"issue":"8","key":"pone.0313444.ref016","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1016\/j.cub.2020.02.023","article-title":"Critical Roles of a RhoGEF-Anillin Module in Septin Architectural Remodeling during Cytokinesis","volume":"30","author":"X Chen","year":"2020","journal-title":"Current Biology"},{"issue":"5","key":"pone.0313444.ref017","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1083\/jcb.142.5.1301","article-title":"Involvement of an Actomyosin Contractile Ring in Saccharomyces cerevisiae Cytokinesis","volume":"142","author":"E Bi","year":"1998","journal-title":"J Cell Biol"},{"issue":"7","key":"pone.0313444.ref018","doi-asserted-by":"crossref","first-page":"1947","DOI":"10.1083\/jcb.143.7.1947","article-title":"Dual Function of Cyk2, a cdc15\/PSTPIP Family Protein, in Regulating Actomyosin Ring Dynamics and Septin Distribution","volume":"143","author":"J Lippincott","year":"1998","journal-title":"J Cell Biol"},{"issue":"5682","key":"pone.0313444.ref019","first-page":"393","article-title":"Spatial Coordination of Cytokinetic Events by Compartmentalization of the Cell Cortex","volume":"305","author":"J Dobbelaere","year":"2004","journal-title":"Science (1979)"},{"issue":"2","key":"pone.0313444.ref020","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1534\/genetics.111.132886","article-title":"Cell polarization and cytokinesis in budding yeast","volume":"191","author":"E Bi","year":"2012","journal-title":"Genetics"},{"issue":"8","key":"pone.0313444.ref021","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/S0960-9822(03)00247-1","article-title":"Septins Have a Dual Role in Controlling Mitotic Exit in Budding Yeast","volume":"13","author":"GA Castillon","year":"2003","journal-title":"Current Biology"},{"issue":"5","key":"pone.0313444.ref022","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1083\/jcb.200312070","article-title":"Septin collar formation in budding yeast requires GTP binding and direct phosphorylation by the PAK, Cla4","volume":"164","author":"M Versele","year":"2004","journal-title":"Journal of Cell Biology"},{"issue":"8\u20139","key":"pone.0313444.ref023","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1515\/BC.2011.083","article-title":"Evidence that a septin diffusion barrier is dispensable for cytokinesis in budding yeast","volume":"392","author":"C Wloka","year":"2011","journal-title":"bchm"},{"issue":"NOV","key":"pone.0313444.ref024","first-page":"1","article-title":"Septin organization and functions in budding yeast","volume":"4","author":"O Glomb","year":"2016","journal-title":"Front Cell Dev Biol"},{"issue":"3","key":"pone.0313444.ref025","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1083\/jcb.132.3.399","article-title":"Identification of a developmentally regulated septin and involvement of the septins in spore formation in Saccharomyces cerevisiae","volume":"132","author":"H Fares","year":"1996","journal-title":"J Cell Biol"},{"issue":"10","key":"pone.0313444.ref026","doi-asserted-by":"crossref","first-page":"2897","DOI":"10.1099\/13500872-142-10-2897","article-title":"SPR28, a sixth member of the septin gene family in Saccharomyces cerevisiae that is expressed specifically in sporulating cells","volume":"142","author":"C De Virgilio","year":"1996","journal-title":"Microbiology (Reading)"},{"issue":"2","key":"pone.0313444.ref027","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.cub.2008.12.030","article-title":"Septin-Mediated Uniform Bracing of Phospholipid Membranes","volume":"19","author":"Y Tanaka-Takiguchi","year":"2009","journal-title":"Current Biology"},{"issue":"6","key":"pone.0313444.ref028","doi-asserted-by":"crossref","first-page":"2146","DOI":"10.1073\/pnas.1314138111","article-title":"Septin assemblies form by diffusion-driven annealing on membranes","volume":"111","author":"AA Bridges","year":"2014","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"pone.0313444.ref029","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1083\/jcb.201512029","article-title":"Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton","volume":"213","author":"AA Bridges","year":"2016","journal-title":"Journal of Cell Biology"},{"key":"pone.0313444.ref030","doi-asserted-by":"crossref","unstructured":"Yi-Wei Huang, Ming Yan, Richard F. Collins, Jessica E. DiCiccio, Sergio Grinstein and WST. Mammalian Septins Are Required for Phagosome Formation. Mol Biol Cell. 2008;19(April):1717\u201326.","DOI":"10.1091\/mbc.e07-07-0641"},{"issue":"9","key":"pone.0313444.ref031","doi-asserted-by":"crossref","first-page":"5280","DOI":"10.1074\/jbc.M114.616201","article-title":"Septin dynamics are essential for exocytosis","volume":"290","author":"E Tokhtaeva","year":"2015","journal-title":"Journal of Biological Chemistry"},{"key":"pone.0313444.ref032","first-page":"1","article-title":"A Septin Diffusion Barrier at the Base of the Primary Cilium Maintains Ciliary Membrane Protein Distribution","volume":"23","author":"Hu Qicong","year":"2011","journal-title":"Science (1979)"},{"issue":"10","key":"pone.0313444.ref033","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.cub.2011.03.067","article-title":"Septins","volume":"21","author":"MP Estey","year":"2011","journal-title":"Current Biology"},{"issue":"11","key":"pone.0313444.ref034","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0013799","article-title":"A Draft of the Human Septin Interactome","volume":"5","author":"M Nakahira","year":"2010","journal-title":"PLoS One"},{"key":"pone.0313444.ref035","article-title":"Septins, a cytoskeletal protein family, with emerging role in striated muscle","author":"M G\u00f6nczi","year":"2020","journal-title":"J Muscle Res Cell Motil"},{"key":"pone.0313444.ref036","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fcell.2017.00003","article-title":"The Mammalian Septin Interactome","volume":"5","author":"K Neubauer","year":"2017","journal-title":"Front Cell Dev Biol"},{"issue":"4","key":"pone.0313444.ref037","doi-asserted-by":"crossref","DOI":"10.1242\/jcs.209098","article-title":"Septins are involved at the early stages of macroautophagy in S. cerevisiae","volume":"131","author":"G Barve","year":"2018","journal-title":"J Cell Sci"},{"issue":"5","key":"pone.0313444.ref038","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1007\/s00294-018-0834-8","article-title":"Septin localization and function during autophagy","volume":"64","author":"G Barve","year":"2018","journal-title":"Curr Genet"},{"issue":"2","key":"pone.0313444.ref039","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0148340","article-title":"Identification of cell cycle dependent interaction partners of the septins by quantitative mass spectrometry","volume":"11","author":"C Renz","year":"2016","journal-title":"PLoS One"},{"issue":"11","key":"pone.0313444.ref040","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.1128\/EC.05087-11","article-title":"Calcineurin Colocalizes with P-Bodies and Stress Granules during Thermal Stress in Cryptococcus neoformans","volume":"10","author":"L Kozubowski","year":"2011","journal-title":"Eukaryot Cell"},{"issue":"1","key":"pone.0313444.ref041","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1006\/fgbi.1999.1180","article-title":"Gene Disruption by Biolistic Transformation in Serotype D Strains of Cryptococcus neoformans","volume":"29","author":"RC Davidson","year":"2000","journal-title":"Fungal Genetics and Biology"},{"key":"pone.0313444.ref042","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/978-1-0716-3722-7_5","volume-title":"Cryptococcus neoformans: Methods and Protocols, Methods in Molecular Biology","author":"Toffaletti Dena L.","year":"2024"},{"issue":"12","key":"pone.0313444.ref043","doi-asserted-by":"crossref","first-page":"3305","DOI":"10.1128\/jcm.31.12.3305-3309.1993","article-title":"Karyotyping of Cryptococcus neoformans as an Epidemiological Tool","volume":"31","author":"JR Perfect","year":"1993","journal-title":"J Clin Microbiol"},{"issue":"3","key":"pone.0313444.ref044","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0174771","article-title":"Time-and radiation-dose dependent changes in the plasma proteome after total body irradiation of non-human primates: Implications for biomarker selection","volume":"12","author":"SD Byrum","year":"2017","journal-title":"PLoS One"},{"issue":"10","key":"pone.0313444.ref045","doi-asserted-by":"crossref","first-page":"3384","DOI":"10.1021\/acs.jproteome.8b00288","article-title":"Label-Free Proteomic Approach to Characterize Protease-Dependent and -Independent Effects of sarA Inactivation on the Staphylococcus aureus Exoproteome","volume":"17","author":"SD Byrum","year":"2018","journal-title":"J Proteome Res"},{"issue":"2","key":"pone.0313444.ref046","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1093\/toxsci\/kfz222","article-title":"Identification of Serum Biomarkers to Distinguish Hazardous and Benign Aminotransferase Elevations","volume":"173","author":"JH Vazquez","year":"2019","journal-title":"Toxicological Sciences"},{"issue":"1","key":"pone.0313444.ref047","doi-asserted-by":"crossref","first-page":"5128","DOI":"10.1038\/s41467-018-07454-w","article-title":"Chromatogram libraries improve peptide detection and quantification by data independent acquisition mass spectrometry","volume":"9","author":"BC Searle","year":"2018","journal-title":"Nat Commun"},{"issue":"4","key":"pone.0313444.ref048","doi-asserted-by":"crossref","first-page":"e1004261","DOI":"10.1371\/journal.pgen.1004261","article-title":"Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation","volume":"10","author":"G Janbon","year":"2014","journal-title":"PLoS Genet"},{"issue":"85","key":"pone.0313444.ref049","doi-asserted-by":"crossref","first-page":"5184","DOI":"10.21105\/joss.05184","article-title":"proteoDA: a package for quantitative proteomics","volume":"8","author":"TJ Thurman","year":"2023","journal-title":"J Open Source Softw"},{"issue":"7","key":"pone.0313444.ref050","doi-asserted-by":"crossref","first-page":"e47","DOI":"10.1093\/nar\/gkv007","article-title":"limma powers differential expression analyses for RNA-sequencing and microarray studies","volume":"43","author":"ME Ritchie","year":"2015","journal-title":"Nucleic Acids Res"},{"issue":"W1","key":"pone.0313444.ref051","doi-asserted-by":"crossref","first-page":"W147","DOI":"10.1093\/nar\/gkw419","article-title":"Heatmapper: web-enabled heat mapping for all","volume":"44","author":"S Babicki","year":"2016","journal-title":"Nucleic Acids Res"},{"issue":"43","key":"pone.0313444.ref052","doi-asserted-by":"crossref","first-page":"15545","DOI":"10.1073\/pnas.0506580102","article-title":"Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles","volume":"102","author":"A Subramanian","year":"2005","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"4","key":"pone.0313444.ref053","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1093\/bioinformatics\/btg455","article-title":"FatiGO: a web tool for finding significant associations of Gene Ontology terms with groups of genes","volume":"20","author":"F Al-Shahrour","year":"2004","journal-title":"Bioinformatics"},{"issue":"7","key":"pone.0313444.ref054","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"CA Schneider","year":"2012","journal-title":"Nat Methods"},{"issue":"7","key":"pone.0313444.ref055","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"J Schindelin","year":"2012","journal-title":"Nat Methods"},{"issue":"6","key":"pone.0313444.ref056","doi-asserted-by":"crossref","first-page":"1869","DOI":"10.1534\/g3.119.400197","article-title":"An interaction network of the human SEPT9 established by quantitative mass spectrometry","volume":"9","author":"M Hecht","year":"2019","journal-title":"G3: Genes, Genomes, Genetics"},{"issue":"D1","key":"pone.0313444.ref057","doi-asserted-by":"crossref","first-page":"D638","DOI":"10.1093\/nar\/gkac1000","article-title":"The STRING database in 2023: protein\u2013protein association networks and functional enrichment analyses for any sequenced genome of interest","volume":"51","author":"D Szklarczyk","year":"2023","journal-title":"Nucleic Acids Res"},{"issue":"20","key":"pone.0313444.ref058","doi-asserted-by":"crossref","first-page":"23102","DOI":"10.1021\/acsami.1c22734","article-title":"Highly Specific Protein Identification by Immunoprecipitation\u2013Mass Spectrometry Using Antifouling Microbeads","volume":"14","author":"E Van Andel","year":"2022","journal-title":"ACS Appl Mater Interfaces"},{"issue":"1","key":"pone.0313444.ref059","doi-asserted-by":"crossref","first-page":"e28","DOI":"10.1093\/nar\/gks941","article-title":"Stoichiometry of chromatin-associated protein complexes revealed by label-free quantitative mass spectrometry-based proteomics","volume":"41","author":"AH Smits","year":"2013","journal-title":"Nucleic Acids Res"},{"issue":"3","key":"pone.0313444.ref060","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/0022-2836(77)90166-8","article-title":"Actin polymerizability is influenced by profilin, a low molecular weight protein in non-muscle cells","volume":"115","author":"L Carlsson","year":"1977","journal-title":"J Mol Biol"},{"issue":"2","key":"pone.0313444.ref061","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1046\/j.1365-2958.2003.03281.x","article-title":"Identification and characterization of a Cryptococcus neoformans ATP binding cassette (ABC) transporter\u2010encoding gene, CnAFR1, involved in the resistance to fluconazole","volume":"47","author":"B Posteraro","year":"2003","journal-title":"Mol Microbiol"},{"issue":"2","key":"pone.0313444.ref062","doi-asserted-by":"crossref","first-page":"1352","DOI":"10.1128\/IAI.74.2.1352-1359.2006","article-title":"Role of AFR1, an ABC Transporter-Encoding Gene, in the In Vivo Response to Fluconazole and Virulence of Cryptococcus neoformans","volume":"74","author":"M Sanguinetti","year":"2006","journal-title":"Infect Immun"},{"issue":"3","key":"pone.0313444.ref063","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1111\/j.1462-5822.2008.01273.x","article-title":"Signalling pathways in the pathogenesis of Cryptococcus","volume":"11","author":"L Kozubowski","year":"2009","journal-title":"Cell Microbiol"},{"issue":"1","key":"pone.0313444.ref064","doi-asserted-by":"crossref","DOI":"10.1093\/genetics\/iyaa009","article-title":"Genetic contribution to high temperature tolerance in Cryptococcus neoformans","volume":"217","author":"PR Stempinski","year":"2021","journal-title":"Genetics"},{"issue":"46","key":"pone.0313444.ref065","doi-asserted-by":"crossref","first-page":"E4393","DOI":"10.1073\/pnas.1318100110","article-title":"Yeast metabolic and signaling genes are required for heat-shock survival and have little overlap with the heat-induced genes","volume":"110","author":"PA Gibney","year":"2013","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2\u20133","key":"pone.0313444.ref066","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/S0014-5793(96)01259-8","article-title":"Profilin is required for the normal timing of actin polymerization in response to thermal stress","volume":"398","author":"J Yeh","year":"1996","journal-title":"FEBS Lett"},{"issue":"3","key":"pone.0313444.ref067","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1083\/jcb.201502062","article-title":"Cytoskeletal dynamics: A view from the membrane","volume":"209","author":"M Bezanilla","year":"2015","journal-title":"Journal of Cell Biology"},{"issue":"3","key":"pone.0313444.ref068","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.bbamem.2006.12.012","article-title":"Profilin binding to sub-micellar concentrations of phosphatidylinositol (4,5) bisphosphate and phosphatidylinositol (3,4,5) trisphosphate","volume":"1768","author":"PDJ Moens","year":"2007","journal-title":"Biochimica et Biophysica Acta (BBA)\u2014Biomembranes"},{"issue":"1","key":"pone.0313444.ref069","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/1471-2091-3-12","article-title":"Mutational analysis of human profilin I reveals a second PI(4,5)-P2 binding site neighbouring the poly(L-proline) binding site","volume":"3","author":"A Lambrechts","year":"2002","journal-title":"BMC Biochem"},{"issue":"44","key":"pone.0313444.ref070","doi-asserted-by":"crossref","first-page":"14027","DOI":"10.1021\/bi961878z","article-title":"Lipid Products of Phosphoinositide 3-Kinase Bind Human Profilin with High Affinity","volume":"35","author":"PJ Lu","year":"1996","journal-title":"Biochemistry"},{"issue":"3","key":"pone.0313444.ref071","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1002\/jcb.240370302","article-title":"Specificity of the interaction between phosphatidylinositol 4,5-bisphosphate and the profilin:actin complex","volume":"37","author":"I Lassing","year":"1988","journal-title":"J Cell Biochem"},{"issue":"6010","key":"pone.0313444.ref072","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1038\/314472a0","article-title":"Specific interaction between phosphatidylinositol 4,5-bisphosphate and profilactin","volume":"314","author":"I Lassing","year":"1985","journal-title":"Nature"},{"issue":"4998","key":"pone.0313444.ref073","first-page":"1231","article-title":"Regulation of Phospholipase C-\u03b31 by Profilin and Tyrosine Phosphorylation","volume":"251","author":"PJ Goldschmidt-Clermont","year":"1991","journal-title":"Science (1979)"},{"issue":"8","key":"pone.0313444.ref074","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1038\/ncb834","article-title":"An actin nucleation mechanism mediated by Bni1 and profilin","volume":"4","author":"I Sagot","year":"2002","journal-title":"Nat Cell Biol"},{"issue":"24","key":"pone.0313444.ref075","doi-asserted-by":"crossref","first-page":"8083","DOI":"10.1523\/JNEUROSCI.0543-14.2014","article-title":"Profilin 1 Associates with Stress Granules and ALS-Linked Mutations Alter Stress Granule Dynamics","volume":"34","author":"MD Figley","year":"2014","journal-title":"Journal of Neuroscience"},{"issue":"4","key":"pone.0313444.ref076","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1002\/yea.3681","article-title":"Yeast stress granules at a glance","volume":"39","author":"T Grousl","year":"2022","journal-title":"Yeast"},{"issue":"7","key":"pone.0313444.ref077","doi-asserted-by":"crossref","first-page":"e0182059","DOI":"10.1371\/journal.pone.0182059","article-title":"Effects of oxidative and thermal stresses on stress granule formation in human induced pluripotent stem cells","volume":"12","author":"F Palangi","year":"2017","journal-title":"PLoS One"},{"key":"pone.0313444.ref078","first-page":"263","author":"AC Fan","year":"2016","journal-title":"RNA Granules and Diseases: A Case Study of Stress Granules in ALS and FTLD. In"},{"issue":"9","key":"pone.0313444.ref079","doi-asserted-by":"crossref","first-page":"e1005873","DOI":"10.1371\/journal.ppat.1005873","article-title":"Calcineurin Targets Involved in Stress Survival and Fungal Virulence","volume":"12","author":"HS Park","year":"2016","journal-title":"PLoS Pathog"},{"issue":"8","key":"pone.0313444.ref080","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1016\/j.bbamcr.2007.05.003","article-title":"Function and regulation in MAPK signaling pathways: Lessons learned from the yeast Saccharomyces cerevisiae","volume":"1773","author":"RE Chen","year":"2007","journal-title":"Biochimica et Biophysica Acta (BBA)\u2014Molecular Cell Research"},{"issue":"1","key":"pone.0313444.ref081","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1152\/physrev.1999.79.1.143","article-title":"Mitogen-Activated Protein Kinase: Conservation of a Three-Kinase Module From Yeast to Human","volume":"79","author":"C WIDMANN","year":"1999","journal-title":"Physiol Rev"},{"issue":"15","key":"pone.0313444.ref082","doi-asserted-by":"crossref","DOI":"10.1242\/jcs.258341","article-title":"Spatiotemporal control of pathway sensors and cross-pathway feedback regulate a differentiation MAPK pathway in yeast","volume":"134","author":"A Prabhakar","year":"2021","journal-title":"J Cell Sci"},{"issue":"7","key":"pone.0313444.ref083","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.2217\/fmb.10.72","article-title":"MAPK cell-cycle regulation in Saccharomyces cerevisiae and Candida albicans","volume":"5","author":"I Correia","year":"2010","journal-title":"Future Microbiol"},{"issue":"2","key":"pone.0313444.ref084","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1093\/genetics\/156.2.579","article-title":"Suppression of the Profilin-Deficient Phenotype by the RHO2 Signaling Pathway in Saccharomyces cerevisiae","volume":"156","author":"N Marcoux","year":"2000","journal-title":"Genetics"},{"issue":"3","key":"pone.0313444.ref085","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1073\/pnas.84.3.779","article-title":"Characterization of two members of the rho gene family from the yeast Saccharomyces cerevisiae","volume":"84","author":"P Madaule","year":"1987","journal-title":"Proc Natl Acad Sci U S A"},{"key":"pone.0313444.ref086","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1146\/annurev.micro.52.1.687","article-title":"Cell polarity and morphogenesis in budding yeast","volume":"52","author":"K Madden","year":"1998","journal-title":"Annu Rev Microbiol"},{"issue":"20","key":"pone.0313444.ref087","doi-asserted-by":"crossref","first-page":"3569","DOI":"10.1242\/jcs.112.20.3569","article-title":"Schizosaccharomyces pombe protein kinase C homologues, pck1p and pck2p, are targets of rho1p and rho2p and differentially regulate cell integrity","volume":"112","author":"M Arellano","year":"1999","journal-title":"J Cell Sci"},{"issue":"2","key":"pone.0313444.ref088","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/j.bbrc.2003.10.140","article-title":"Role of guanine nucleotide exchange factors for Rho family GTPases in the regulation of cell morphology and actin cytoskeleton in fission yeast","volume":"312","author":"N Iwaki","year":"2003","journal-title":"Biochem Biophys Res Commun"},{"issue":"15","key":"pone.0313444.ref089","doi-asserted-by":"crossref","first-page":"11516","DOI":"10.1074\/jbc.M109.071027","article-title":"Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating protein","volume":"285","author":"T Soto","year":"2010","journal-title":"J Biol Chem"},{"issue":"12","key":"pone.0313444.ref090","doi-asserted-by":"crossref","first-page":"5028","DOI":"10.1091\/mbc.e06-08-0688","article-title":"Rho2 Is a Target of the Farnesyltransferase Cpp1 and Acts Upstream of Pmk1 Mitogen-activated Protein Kinase Signaling in Fission Yeast","volume":"17","author":"Y Ma","year":"2006","journal-title":"Mol Biol Cell"},{"issue":"14","key":"pone.0313444.ref091","doi-asserted-by":"crossref","first-page":"2745","DOI":"10.1128\/MCB.01515-13","article-title":"Rho2 Palmitoylation Is Required for Plasma Membrane Localization and Proper Signaling to the Fission Yeast Cell Integrity Mitogen-Activated Protein Kinase Pathway","volume":"34","author":"L S\u00e1nchez-Mir","year":"2014","journal-title":"Mol Cell Biol"},{"issue":"18","key":"pone.0313444.ref092","doi-asserted-by":"crossref","first-page":"2295","DOI":"10.1101\/gad.11.18.2295","article-title":"GTPases and signaling networks","volume":"11","author":"L Van Aelst","year":"1997","journal-title":"Genes Dev"},{"issue":"1","key":"pone.0313444.ref093","doi-asserted-by":"crossref","first-page":"22184","DOI":"10.1038\/s41598-020-78470-4","article-title":"A Rab escort protein regulates the MAPK pathway that controls filamentous growth in yeast","volume":"10","author":"S Jamalzadeh","year":"2020","journal-title":"Sci Rep"},{"issue":"15","key":"pone.0313444.ref094","doi-asserted-by":"crossref","first-page":"3151","DOI":"10.1093\/nar\/22.15.3151","article-title":"LRG1 is expressed during sporulation in Saccharomyces cerevisiae and contains motifs similar to LIM and rho\/racGAP domains","volume":"22","author":"L M\u00fcller","year":"1994","journal-title":"Nucleic Acids Res"},{"issue":"3","key":"pone.0313444.ref095","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1007\/s004380100580","article-title":"Lrg1p functions as a putative GTPase-activating protein in the Pkc1p-mediated cell integrity pathway in Saccharomyces cerevisiae","volume":"266","author":"A Lorberg","year":"2001","journal-title":"Mol Genet Genomics"},{"issue":"10","key":"pone.0313444.ref096","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1002\/yea.742","article-title":"Yeast Lrg1p acts as a specialized RhoGAP regulating 1,3-beta-glucan synthesis","volume":"18","author":"D Watanabe","year":"2001","journal-title":"Yeast"},{"issue":"5","key":"pone.0313444.ref097","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.cub.2008.02.005","article-title":"Pom1 DYRK regulates localization of the Rga4 GAP to ensure bipolar activation of Cdc42 in fission yeast","volume":"18","author":"H Tatebe","year":"2008","journal-title":"Curr Biol"},{"issue":"10","key":"pone.0313444.ref098","doi-asserted-by":"crossref","first-page":"e1006405","DOI":"10.1371\/journal.pgen.1006405","article-title":"Signaling through Lrg1, Rho1 and Pkc1 Governs Candida albicans Morphogenesis in Response to Diverse Cues","volume":"12","author":"JL Xie","year":"2016","journal-title":"PLoS Genet"},{"issue":"5","key":"pone.0313444.ref099","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01767-19","article-title":"Lrg1 Regulates \u03b2 (1,3)-Glucan Masking in Candida albicans through the Cek1 MAP Kinase Pathway","volume":"10","author":"T Chen","year":"2019","journal-title":"mBio"},{"key":"pone.0313444.ref100","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.01085","article-title":"The role of Cdc42 and Gic1 in the regulation of septin filament formation and dissociation","volume":"2","author":"Y Sadian","year":"2013","journal-title":"Elife"},{"issue":"3","key":"pone.0313444.ref101","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1111\/j.1365-2958.2009.07019.x","article-title":"Two CDC42 paralogues modulate Cryptococcus neoformans thermotolerance and morphogenesis under host physiological conditions","volume":"75","author":"ER Ballou","year":"2010","journal-title":"Mol Microbiol"},{"issue":"8","key":"pone.0313444.ref102","doi-asserted-by":"crossref","first-page":"e1003687","DOI":"10.1371\/journal.pgen.1003687","article-title":"Ras1 Acts through Duplicated Cdc42 and Rac Proteins to Regulate Morphogenesis and Pathogenesis in the Human Fungal Pathogen Cryptococcus neoformans","volume":"9","author":"ER Ballou","year":"2013","journal-title":"PLoS Genet"},{"issue":"3","key":"pone.0313444.ref103","doi-asserted-by":"crossref","first-page":"161","DOI":"10.4489\/MYCO.2009.37.3.161","article-title":"The Stress-Activated Signaling (SAS) Pathways of a Human Fungal Pathogen, Cryptococcus neoformans","volume":"37","author":"KW Jung","year":"2009","journal-title":"Mycobiology"},{"issue":"10","key":"pone.0313444.ref104","article-title":"Cell Wall Integrity Pathway Involved in Morphogenesis, Virulence and Antifungal Susceptibility in Cryptococcus neoformans","volume":"7","author":"HC de Oliveira","year":"2021","journal-title":"J Fungi (Basel)"},{"issue":"2","key":"pone.0313444.ref105","doi-asserted-by":"crossref","first-page":"e1000752","DOI":"10.1371\/journal.ppat.1000752","article-title":"An Extensive Circuitry for Cell Wall Regulation in Candida albicans","volume":"6","author":"JR Blankenship","year":"2010","journal-title":"PLoS 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transporters","volume":"7","author":"S Wilkens","year":"2015","journal-title":"F1000Prime Rep"},{"issue":"1","key":"pone.0313444.ref110","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1038\/s42005-023-01320-y","article-title":"ABC transporters are billion-year-old Maxwell Demons","volume":"6","author":"S Flatt","year":"2023","journal-title":"Commun Phys"},{"issue":"4","key":"pone.0313444.ref111","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.01751-17","article-title":"Roles of Three Cryptococcus neoformans and Cryptococcus gattii Efflux Pump-Coding Genes in Response to Drug Treatment","volume":"62","author":"M Chang","year":"2018","journal-title":"Antimicrob Agents Chemother"},{"issue":"7","key":"pone.0313444.ref112","doi-asserted-by":"crossref","first-page":"2804","DOI":"10.1128\/AAC.00295-09","article-title":"Heteroresistance to Fluconazole in Cryptococcus neoformans Is Intrinsic and Associated with Virulence","volume":"53","author":"E Sionov","year":"2009","journal-title":"Antimicrob Agents Chemother"},{"issue":"2","key":"pone.0313444.ref113","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1111\/j.1567-1364.2008.00470.x","article-title":"The ABC transporter-encoding gene AFR1 affects the resistance of Cryptococcus neoformans to microglia-mediated antifungal activity by delaying phagosomal maturation","volume":"9","author":"CF Orsi","year":"2009","journal-title":"FEMS Yeast Res"},{"issue":"7","key":"pone.0313444.ref114","doi-asserted-by":"crossref","first-page":"677","DOI":"10.3390\/jof8070677","article-title":"Novel ABC Transporter Associated with Fluconazole Resistance in Aging of Cryptococcus neoformans","volume":"8","author":"NK Oliveira","year":"2022","journal-title":"Journal of Fungi"},{"issue":"11","key":"pone.0313444.ref115","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1091\/mbc.E20-01-0006","article-title":"Importin binding mediates the intramolecular regulation of anillin during cytokinesis","volume":"31","author":"D Beaudet","year":"2020","journal-title":"Mol Biol Cell"},{"issue":"6","key":"pone.0313444.ref116","doi-asserted-by":"crossref","first-page":"106903","DOI":"10.1016\/j.isci.2023.106903","article-title":"The Rho1 GTPase controls anillo-septin assembly to facilitate contractile ring closure during cytokinesis","volume":"26","author":"SC Carim","year":"2023","journal-title":"iScience"},{"issue":"1","key":"pone.0313444.ref117","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.cub.2011.11.040","article-title":"Cleavage Furrow Organization Requires PIP2-Mediated Recruitment of Anillin","volume":"22","author":"J Liu","year":"2012","journal-title":"Current Biology"},{"issue":"3","key":"pone.0313444.ref118","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1083\/jcb.150.3.539","article-title":"Functional Analysis of a Human Homologue of the Drosophila Actin Binding Protein Anillin Suggests a Role in Cytokinesis","volume":"150","author":"K Oegema","year":"2000","journal-title":"J Cell Biol"},{"issue":"4","key":"pone.0313444.ref119","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.devcel.2015.03.003","article-title":"Mechanistic Insights into the Anchorage of the Contractile Ring by Anillin and Mid1","volume":"33","author":"L Sun","year":"2015","journal-title":"Dev Cell"},{"key":"pone.0313444.ref120","doi-asserted-by":"crossref","first-page":"103697","DOI":"10.1016\/j.fgb.2022.103697","article-title":"Role of the anillin-like protein in growth of Cryptococcus neoformans at human host temperature","volume":"160","author":"CA Peng","year":"2022","journal-title":"Fungal Genetics and Biology"},{"issue":"7","key":"pone.0313444.ref121","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1083\/jcb.200211126","article-title":"An anillin homologue, Mid2p, acts during fission yeast cytokinesis to organize the septin ring and promote cell separation","volume":"160","author":"JJ Tasto","year":"2003","journal-title":"J Cell Biol"},{"issue":"5","key":"pone.0313444.ref122","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1242\/jcs.118521","article-title":"Coupling of septins to the axial landmark by Bud4 in budding yeast","volume":"126","author":"PJ Kang","year":"2013","journal-title":"J Cell Sci"},{"issue":"3","key":"pone.0313444.ref123","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1128\/EC.00268-14","article-title":"The anillin-related region of Bud4 is the major functional determinant for Bud4\u2019s function in septin organization during bud growth and axial bud site selection in budding yeast","volume":"14","author":"H Wu","year":"2015","journal-title":"Eukaryot Cell"},{"issue":"2","key":"pone.0313444.ref124","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1128\/MCB.25.2.767-778.2005","article-title":"Genetic Analysis of the Kinetochore DASH Complex Reveals an Antagonistic Relationship 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