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This retrospective analysis of six years of routinely collected respiratory pathogen multiplex PCR testing at a major paediatric hospital in New South Wales Australia, describes the epidemiology, year-round seasonality, and co-detection patterns of 15 viral respiratory pathogens. 32,599 respiratory samples from children aged under 16 years were analysed. Most samples were associated with a hospital admission (24,149, 74.1%) and the median age of sampling was 16 months (IQR 5\u201353). Viruses were detected in 62.9% (20,510) of samples, with single virus detections occurring in 73.5% (15,082) of positive samples. In instances of single virus detection, rhinovirus was most frequent (5125, 40.6%), followed by RSV-B (1394, 9.2%) and RSV-A (1290, 8.6%). Moderate to strong seasonal strength was observed for most viruses with some notable exceptions. Rhinovirus and enterovirus were detected year-round and low seasonal strength was observed for adenovirus and bocavirus. Biennial seasonal patterns were observed for influenza B and parainfluenza virus 2. Co-detections occurred in 5,428 samples, predominantly with two (4284, 79.0%) or three viruses (904, 16.7%). The most common co-detections were rhinovirus-adenovirus (566, 10.4%), rhinovirus-enterovirus (357, 8.3%), and rhinovirus-RSV-B (337, 7.9%). Ongoing pan-pathogen surveillance, integrating both laboratory and clinical data, is necessary to assist in identification of key pathogens and combination of pathogens to support effective preventative public health strategies and reduce the burden of paediatric respiratory infections.<\/jats:p>","DOI":"10.1371\/journal.pone.0313504","type":"journal-article","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T13:35:49Z","timestamp":1737552949000},"page":"e0313504","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Respiratory virus detections in children presenting to an Australian paediatric referral hospital pre-COVID-19 pandemic, January 2014 to December 2019"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0377-9641","authenticated-orcid":true,"given":"Rebecca","family":"Burrell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0040-6679","authenticated-orcid":true,"given":"Gemma L.","family":"Saravanos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison","family":"Kesson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kin-chuen","family":"Leung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0175-5769","authenticated-orcid":true,"given":"Alex C.","family":"Outhred","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Wood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2391-4396","authenticated-orcid":true,"given":"David","family":"Muscatello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip N.","family":"Britton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,1,22]]},"reference":[{"key":"pone.0313504.ref001","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1016\/S0140-6736(20)30925-9","article-title":"Global burden of 369 diseases and injuries in 204 countries and territories, 1990\u20132019: a systematic analysis for the Global Burden of Disease Study 2019","volume":"396","author":"T Vos","year":"2020","journal-title":"The Lancet"},{"key":"pone.0313504.ref002","unstructured":"Australian Institute of Health and Welfare. 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