{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T15:04:32Z","timestamp":1781708672754,"version":"3.54.5"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2024,11,21]],"date-time":"2024-11-21T00:00:00Z","timestamp":1732147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shenzhen High-level Hospital Construction Fund","award":["YBH2019-260"],"award-info":[{"award-number":["YBH2019-260"]}]},{"name":"Shenzhen Key Medical Discipline Construction Fund","award":["SZXK027"],"award-info":[{"award-number":["SZXK027"]}]},{"DOI":"10.13039\/501100012151","name":"Sanming Project of Medicine in Shenzhen","doi-asserted-by":"crossref","award":["SZSM202011016"],"award-info":[{"award-number":["SZSM202011016"]}],"id":[{"id":"10.13039\/501100012151","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Context: Estrogen receptor \u03b1 (ER\u03b1) is a key regulator of reproductive function, particularly in ovarian development and function, yet the specifics of its role at the molecular level remain unclear. Aims: The study aims to elucidate the molecular mechanisms of ER\u03b1-regulated transcriptional dynamics in ovarian cells using ER\u03b1 knockout (\u03b1ERKO) mice created via CRISPR\/Cas9. Methods: Single-cell RNA sequencing (scRNA-seq) was used to compare transcriptomes from individual ovarian cells in both wild type and \u03b1ERKO mice. Bioinformatics analyses identified distinct cell populations and their transcriptional profiles post ER\u03b1 deletion. Key Results: Distinct oocyte and granulosa cell populations were identified, with ER\u03b1 deletion disrupting the regulation of genes linked to ovarian infertility, the ovulation cycle, and steroidogenesis. Greb1 expression in granulosa cells was found to be ER\u03b1-dependent. Conclusions: ER\u03b1 deletion significantly alters the transcriptional landscape of ovarian cells, affecting genes and pathways central to ovarian function and the ovulation process. Implications: The findings provide an in-depth, single-cell view of ER\u03b1\u2019s role in the reproductive system, offering insights that may lead to novel treatments for ovarian disorders.<\/jats:p>","DOI":"10.1371\/journal.pone.0313867","type":"journal-article","created":{"date-parts":[[2024,11,21]],"date-time":"2024-11-21T18:50:42Z","timestamp":1732215042000},"page":"e0313867","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Single-cell sequencing reveals transcriptional dynamics regulated by ER\u03b1 in mouse 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