{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T19:33:31Z","timestamp":1790105611230,"version":"4.0.1"},"reference-count":40,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T00:00:00Z","timestamp":1731974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["NU23-08-00499"],"award-info":[{"award-number":["NU23-08-00499"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["CZ.02.1.01\/0.0\/0.0\/16_026\/0008451"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_026\/0008451"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Brno City Municipality","award":["Brno PhD Talent Scholarship"],"award-info":[{"award-number":["Brno PhD Talent Scholarship"]}]},{"DOI":"10.13039\/501100011659","name":"L\u00e9ka\u0159sk\u00e1 fakulta, Masarykova univerzita","doi-asserted-by":"publisher","award":["MUNI\/A\/1564\/2023"],"award-info":[{"award-number":["MUNI\/A\/1564\/2023"]}],"id":[{"id":"10.13039\/501100011659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["NU22-08-00124"],"award-info":[{"award-number":["NU22-08-00124"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["NW24-08-00064"],"award-info":[{"award-number":["NW24-08-00064"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Collaterals improve recanalization in acute ischemic stroke patients treated with intravenous thrombolysis, but the mechanisms are poorly understood. To investigate it, an <jats:italic>in vitro<\/jats:italic> flow model of the middle cerebral artery was developed with or without collaterals. An occlusion was achieved using human blood clots. Recanalization time, thrombolysis (clot length decrease and red blood cell (RBC) release), pressure gradient across the clot and clot compaction were measured. Results showed that with or without collateral alteplase-treated RBC dominant clots showed recanalization time 98\u00b123 min vs 130\u00b135 min (difference 32 min, 95% CI -6-58 min), relative clot reduction 31.8\u00b114.9% vs 30.3\u00b113.2% (difference 1.5%, 95% CI 10.4\u201313.4%) and RBC release 0.30\u00b10.07 vs 0.27\u00b10.09 (difference 0.03, 95% CI 0.04\u20130.10). Similar results were observed with fibrin-dominant clots. In RBC dominant clots, the presence vs absence of collateral caused different pressure gradients across the clot 0.41\u00b10.09 vs 0.70\u00b10.09 mmHg (difference 0.29 mmHg, 95% CI -0.17\u20130.41 mmHg), and caused the reduction of initial clot compaction by 5%. These findings align with observations in patients, where collaterals shortened recanalization time. However, collaterals did not increase thrombolysis. Instead, they decreased the pressure gradient across the clot, resulting in less clot compaction and easier distal displacement of the clot.<\/jats:p>","DOI":"10.1371\/journal.pone.0314079","type":"journal-article","created":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T18:26:23Z","timestamp":1732040783000},"page":"e0314079","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["A collateral circulation in ischemic stroke accelerates recanalization due to lower clot compaction"],"prefix":"10.1371","volume":"19","author":[{"given":"Sandra","family":"Thalerov\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"V\u00edte\u010dkov\u00e1 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