{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:42:51Z","timestamp":1792802571868,"version":"4.5.0"},"reference-count":52,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T00:00:00Z","timestamp":1732492800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Office of Research Administration, Chiang Mai University, Chiang Mai 50200","award":["EX010082"],"award-info":[{"award-number":["EX010082"]}],"id":[{"id":"https:\/\/ror.org\/05m2fqn25","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    <jats:italic>Aedes albopictus<\/jats:italic>\n                    , known as the Asian tiger mosquito, is a significant vector for dengue fever, chikungunya, zika virus, yellow fever. Current control methods rely on chemical insecticides, which face challenges such as resistance, environmental harm, and impact on non-target species\n                    <jats:italic>Eudrilus eugeniae<\/jats:italic>\n                    and\n                    <jats:italic>Artemia salina<\/jats:italic>\n                    . This study evaluates the toxic effects of biogenic copper nanoparticles (CuNPs) synthesized using\n                    <jats:italic>Metarhizium robertsii<\/jats:italic>\n                    intracellular extract obtained from our previous research. The CuNPs were tested against\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>albopictus<\/jats:italic>\n                    and non-target species at 24 and 48 hours post-treatment. Results demonstrated that entomopathogenic fungi-derived CuNPs exhibited potent mosquitocidal activity, resulting in 97.33% mortality in larvae, 93.33% in pupae, and 74.66% in adults at 48 hours post-treatment. The CuNPs derived from\n                    <jats:italic>M<\/jats:italic>\n                    .\n                    <jats:italic>robertsii<\/jats:italic>\n                    showed lower LC\n                    <jats:sub>50<\/jats:sub>\n                    values of 74.873 mg\/L in larvae, 76.101 mg\/L in pupae, and 136.645 mg\/L in adults at 48 hours post-treatment. Additionally, 12 hours post-treatment, catalase (an antioxidant enzyme) activity decreased 1.5-fold in a dose-dependent manner, while glutathione S-transferase (a detoxification enzyme) activity increased 7.8-fold. CuNPs demonstrated lower toxicity to non-target species, with 24% mortality in\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>salina<\/jats:italic>\n                    and 24.44% mortality in\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>eugeniae<\/jats:italic>\n                    at 24 hours post-treatment. The LC\n                    <jats:sub>50<\/jats:sub>\n                    values were 634.747 mg\/L for\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>salina<\/jats:italic>\n                    and 602.494 mg\/L for\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>eugeniae<\/jats:italic>\n                    at 24 hours post-treatment. These findings indicate that entomopathogenic fungi-derived CuNPs are a promising, target-specific candidate for controlling\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>albopictus<\/jats:italic>\n                    at various life stages (larvae, pupae, and adults).\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0314279","type":"journal-article","created":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T13:34:48Z","timestamp":1732541688000},"page":"e0314279","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Effects of copper nanoparticles synthesized from the entomopathogen Metarhizium robertsii against the dengue vector Aedes albopictus (Skuse, 1894)"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3283-1306","authenticated-orcid":true,"given":"Perumal","family":"Vivekanandhan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kannan","family":"Swathy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pittarate","family":"Sarayut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3521-7659","authenticated-orcid":true,"given":"Patcharin","family":"Krutmuang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,11,25]]},"reference":[{"key":"pone.0314279.ref001","doi-asserted-by":"crossref","first-page":"1332334","DOI":"10.3389\/fpubh.2023.1332334","article-title":"Aedes albopictus arrives in Lisbon: an emerging public health threat","author":"T Nazareth","year":"2023","journal-title":"Front Pub Health"},{"key":"pone.0314279.ref002","first-page":"101137","article-title":"Chemical constituents and larvicidal efficacy of Naringi crenulata (Rutaceae) plant extracts and bioassay guided fractions against Culex quinquefasciatus mosquito (Diptera: Culicidae). 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