{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:17:16Z","timestamp":1785284236445,"version":"3.55.0"},"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","award":["RSPD2024R971"],"award-info":[{"award-number":["RSPD2024R971"]}],"id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The members of genus <jats:italic>Moniezia<\/jats:italic> are the common parasites of livestock in tropical areas. The tapeworm, <jats:italic>Moniezia expansa<\/jats:italic> is commonly found in the gastrointestinal tract of the small and large ruminants. The present study focused on reporting the prevalence of <jats:italic>M<\/jats:italic>. <jats:italic>expansa<\/jats:italic> in small ruminants of southern Punjab: sheep and goats, in relation with epidemiological factors like age and gender. An overall prevalence of 27.2% was estimated for the small ruminants with higher infection rates in males (29.8%) and younger age group (&lt;1 year; 32.9%). Moreover, the molecular characterization and phylogenetic analysis of the isolates based on partial <jats:italic>cox1<\/jats:italic> gene indicated the placement of these sequences in the <jats:italic>M<\/jats:italic>. <jats:italic>expansa<\/jats:italic> cluster. Two distinct haplotypes, without any host tropism, were identified within the Pakistani isolates. A meta-analysis for <jats:italic>M<\/jats:italic>. <jats:italic>expansa<\/jats:italic> was run for all available global reports exhibiting an overall pooled prevalence of 21.3% (CI 95%: 13.5\u201329.0). Additionally, a global dataset encompassing 59 partial <jats:italic>cox1<\/jats:italic> sequences submitted from different geographical locations was also assessed. Moderate haplotype diversity (0.760 \u00b1 0.051) and significantly negative deviations from neutrality were estimated. The median joining haplotype network for these sequences revealed an interesting population structure indicating highly divergent sequences from China and Iraq compared to Pakistan, India, Vietnam, Senegal and Ethiopia. Given inconsistencies in genetic data there is a dire need to carry out molecular studies across the entire distributional range of <jats:italic>M<\/jats:italic>. <jats:italic>expansa<\/jats:italic> to delineate genetic diversity and population structure of the species. This will also be crucial in reevaluating the taxonomy of genus <jats:italic>Moniezia<\/jats:italic>.<\/jats:p>","DOI":"10.1371\/journal.pone.0314343","type":"journal-article","created":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T19:02:10Z","timestamp":1733425330000},"page":"e0314343","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["First molecular report of Moniezia expansa in small ruminants of Pakistan with epidemiological insight"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8255-1011","authenticated-orcid":true,"given":"Hira","family":"Muqaddas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7852-9113","authenticated-orcid":true,"given":"Naunain","family":"Mehmood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maher","family":"Nigar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2689-7452","authenticated-orcid":true,"given":"Farhana","family":"Yousaf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6968-3358","authenticated-orcid":true,"given":"Kainat Farooq","family":"Khokhar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6132-0297","authenticated-orcid":true,"given":"Saba","family":"Kousar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahnoor","family":"Aslam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zafar Iqbal","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis A.","family":"Giantsis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayman A.","family":"Swelum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4996-0152","authenticated-orcid":true,"given":"Furhan","family":"Iqbal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,12,5]]},"reference":[{"issue":"4","key":"pone.0314343.ref001","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/s12639-013-0259-8","volume":"38","author":"SNK Jyoti","year":"2014","journal-title":"J. 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