{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T16:16:56Z","timestamp":1769012216926,"version":"3.49.0"},"reference-count":100,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T00:00:00Z","timestamp":1733184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Department of Biotechnology, Ministry of Science and Technology, Government of India","award":["BT\/ADV\/Canine Health\/GADVASU\/2017-18"],"award-info":[{"award-number":["BT\/ADV\/Canine Health\/GADVASU\/2017-18"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Evaluation of the effectiveness of vaccination of animals against rabies is not routinely implemented. In cases where it is carried out, the rapid fluorescent focus inhibition test (RFFIT) or the fluorescent antibody virus neutralization (FAVN) test are the recommended tests. However, both of these tests require handling of live rabies virus (RABV), and are cumbersome to perform. In view of this, the enzyme-linked immunosorbent assay (ELISA) has been proposed as a surrogate test; however, availability of appropriate antigen is a major impediment for the development of ELISAs to detect anti-rabies antibodies. The most widely used antigen is the RABV glycoprotein (G) purified from cell culture-propagated virus, which requires a biosafety level 3 containment. The alternative is to use recombinantly expressed G, which needs to be to be properly glycosylated and folded to serve as the best antigen. The most suitable system for its production is the baculovirus expression system (BVES). However, purification of RABV G is challenging. We therefore tested partially purified preparations in the form of extracts of insect cells infected with baculovirus expressing RABV G, against sera from vaccinated dogs in an indirect ELISA. The results showed good concordance against RFFIT, with sensitivity and specificity of 90.48% and 80.00%, respectively. The system may be used for quick screening to determine the presence and an approximate level of antibodies, and can be modified to enable monitoring of mass dog vaccination programs, as well as to facilitate certification of dogs intended for international travel and transportation.<\/jats:p>","DOI":"10.1371\/journal.pone.0314516","type":"journal-article","created":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T18:23:02Z","timestamp":1733250182000},"page":"e0314516","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of the immune status of dogs vaccinated against rabies by an enzyme-linked immunosorbent assay using crude preparations of insect cells infected with a recombinant baculovirus encoding the rabies virus glycoprotein gene"],"prefix":"10.1371","volume":"19","author":[{"given":"A. 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Tarantola","year":"2017","journal-title":"Trop Med Infect Dis"},{"key":"pone.0314516.ref002","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1186\/1743-422X-9-50","article-title":"Rabies molecular virology, diagnosis, prevention and treatment","volume":"9","author":"MZ Yousaf","year":"2012","journal-title":"Virol J"},{"issue":"RR-2","key":"pone.0314516.ref003","first-page":"1","article-title":"Use of a reduced (4-dose) vaccine schedule for postexposure prophylaxis to prevent human rabies: recommendations of the advisory committee on immunization practices","volume":"59","author":"CE Rupprecht","year":"2010","journal-title":"MMWR Recomm Rep"},{"key":"pone.0314516.ref004","unstructured":"WHO. WHO expert consultation on rabies, Third Report. Geneva, Switzerland: World Health Organization, 2018."},{"issue":"11","key":"pone.0314516.ref005","article-title":"Rabies Control and Treatment: From Prophylaxis to Strategies with Curative Potential","volume":"8","author":"S Zhu","year":"2016","journal-title":"Viruses"},{"issue":"12","key":"pone.0314516.ref006","doi-asserted-by":"crossref","first-page":"1912","DOI":"10.2460\/javma.2005.227.1912","article-title":"Rabies surveillance in the United States during 2004","volume":"227","author":"JW Krebs","year":"2005","journal-title":"J Am Vet Med Assoc"},{"key":"pone.0314516.ref007","first-page":"1","article-title":"Rationale and prospects for rabies elimination in developing countries","volume":"187","author":"FX Meslin","year":"1994","journal-title":"Curr Top Microbiol Immunol"},{"issue":"22","key":"pone.0314516.ref008","doi-asserted-by":"crossref","first-page":"1632","DOI":"10.1056\/NEJM199311253292208","article-title":"Rabies","volume":"329","author":"DB Fishbein","year":"1993","journal-title":"N Engl J Med"},{"issue":"Suppl 1","key":"pone.0314516.ref009","doi-asserted-by":"crossref","first-page":"A73","DOI":"10.1016\/j.vaccine.2018.10.012","article-title":"Global characteristics of the rabies biologics market in 2017","volume":"37","author":"A Ives","year":"2019","journal-title":"Vaccine"},{"issue":"3","key":"pone.0314516.ref010","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/0264-410X(95)00197-9","article-title":"Immunization coverage required to prevent outbreaks of dog rabies","volume":"14","author":"PG Coleman","year":"1996","journal-title":"Vaccine"},{"key":"pone.0314516.ref011","doi-asserted-by":"crossref","first-page":"38","DOI":"10.3389\/fvets.2017.00038","article-title":"Cost Description and Comparative Cost Efficiency of Post-Exposure Prophylaxis and Canine Mass Vaccination against Rabies in N\u2019Djamena, Chad","volume":"4","author":"R Mindekem","year":"2017","journal-title":"Front Vet Sci"},{"key":"pone.0314516.ref012","first-page":"417","volume-title":"Laboratory techniques in rabies","author":"EA Fitzgerald","year":"1996","edition":"4"},{"key":"pone.0314516.ref013","doi-asserted-by":"crossref","first-page":"570","DOI":"10.3181\/00379727-32-7767P","article-title":"Early diagnosis of rabies by mouse inoculation. 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Detection in Domestic Canine","volume":"2023","author":"Y Fitria","year":"2023","journal-title":"Vet Med Int"},{"issue":"5","key":"pone.0314516.ref035","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1128\/jcm.27.5.899-902.1989","article-title":"Enzyme-linked immunosorbent assay for determination of antibodies to the envelope glycoprotein of rabies virus","volume":"27","author":"M Grassi","year":"1989","journal-title":"J Clin Microbiol"},{"issue":"4","key":"pone.0314516.ref036","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1292\/jvms.61.411","article-title":"A new quantitative method for rabies virus by detection of nucleoprotein in virion using ELISA","volume":"61","author":"S Katayama","year":"1999","journal-title":"J Vet Med Sci"},{"issue":"5","key":"pone.0314516.ref037","first-page":"422","article-title":"Enzyme-linked immunosorbent assay for quantification of rabies antibodies in human sera","volume":"28","author":"L Kavaklova","year":"1984","journal-title":"Acta Virol"},{"issue":"28","key":"pone.0314516.ref038","doi-asserted-by":"crossref","first-page":"3310","DOI":"10.1016\/j.vaccine.2016.04.086","article-title":"Development and validation of a quantitative competitive ELISA for potency testing of equine anti rabies sera with other potential use","volume":"34","author":"J Korimbocus","year":"2016","journal-title":"Vaccine"},{"issue":"36","key":"pone.0314516.ref039","doi-asserted-by":"crossref","first-page":"5358","DOI":"10.1016\/j.vaccine.2012.06.037","article-title":"Evaluation of a new serological technique for detecting rabies virus antibodies following vaccination","volume":"30","author":"X Ma","year":"2012","journal-title":"Vaccine"},{"issue":"1","key":"pone.0314516.ref040","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1002\/jmv.1890090107","article-title":"Enzyme-linked immunosorbent assay: a rapid reproducible test for the measurement of rabies antibody","volume":"9","author":"KG Nicholson","year":"1982","journal-title":"J Med Virol"},{"issue":"1","key":"pone.0314516.ref041","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1590\/S0036-46651999000100008","article-title":"An ELISA suitable for the detection of rabies virus antibodies in serum samples from human vaccinated with either cell-culture vaccine or suckling-mouse-brain vaccine","volume":"41","author":"AS Piza","year":"1999","journal-title":"Rev Inst Med Trop Sao Paulo"},{"issue":"2","key":"pone.0314516.ref042","doi-asserted-by":"crossref","first-page":"97","DOI":"10.7774\/cevr.2023.12.2.97","article-title":"Detection of immunity in sheep following anti-rabies vaccination","volume":"12","author":"H Rathnadiwakara","year":"2023","journal-title":"Clin Exp Vaccine Res"},{"issue":"1","key":"pone.0314516.ref043","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.jviromet.2009.05.018","article-title":"Development and evaluation of a rapid neutralizing antibody test for rabies","volume":"161","author":"S Shiota","year":"2009","journal-title":"J Virol Methods"},{"issue":"6","key":"pone.0314516.ref044","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1128\/cdli.4.6.727-730.1997","article-title":"A new competitive enzyme-linked immunosorbent assay demonstrates adequate immune levels to rabies virus in compulsorily vaccinated Japanese domestic dogs","volume":"4","author":"M Sugiyama","year":"1997","journal-title":"Clin Diagn Lab Immunol"},{"key":"pone.0314516.ref045","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.jviromet.2016.10.006","article-title":"First international collaborative study to evaluate rabies antibody detection method for use in monitoring the effectiveness of oral vaccination programmes in fox and raccoon dog in Europe","volume":"238","author":"M Wasniewski","year":"2016","journal-title":"J Virol 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I. Functional recombinant protein in stable co-transfected cell line","volume":"2","author":"AY Yokomizo","year":"2007","journal-title":"Biotechnol J"},{"issue":"4","key":"pone.0314516.ref052","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1111\/j.1745-7254.2005.00078.x","article-title":"Baculovirus as a highly efficient expression vector in insect and mammalian cells","volume":"26","author":"YC Hu","year":"2005","journal-title":"Acta Pharmacol Sin"},{"issue":"2\u20133","key":"pone.0314516.ref053","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/BF02788049","article-title":"The use of baculoviruses as expression vectors","volume":"42","author":"IM Kidd","year":"1993","journal-title":"Appl Biochem Biotechnol"},{"issue":"2","key":"pone.0314516.ref054","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/0042-6822(89)90551-5","article-title":"Immunogenic and protective properties of rabies virus glycoprotein expressed by baculovirus vectors","volume":"173","author":"C Prehaud","year":"1989","journal-title":"Virology"},{"issue":"10","key":"pone.0314516.ref055","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1128\/CVI.05258-11","article-title":"Expression and solubilization of insect cell-based rabies virus glycoprotein and assessment of its immunogenicity and protective efficacy in mice","volume":"18","author":"R Ramya","year":"2011","journal-title":"Clin Vaccine Immunol"},{"issue":"1\u20132","key":"pone.0314516.ref056","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0168-1702(92)90095-Q","article-title":"Characterization of rabies virus glycoprotein expressed by recombinant baculovirus","volume":"25","author":"K Tuchiya","year":"1992","journal-title":"Virus Res"},{"issue":"2","key":"pone.0314516.ref057","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1128\/JVI.72.2.1085-1091.1998","article-title":"Neuronal cell surface molecules mediate specific binding to rabies virus glycoprotein expressed by a recombinant baculovirus on the surfaces of lepidopteran cells","volume":"72","author":"C Tuffereau","year":"1998","journal-title":"J Virol"},{"key":"pone.0314516.ref058","first-page":"175","volume-title":"Laboratory techniques in rabies","author":"World Health Organization","year":"1996"},{"key":"pone.0314516.ref059","first-page":"493","article-title":"A simple method of estimating fifty per cent endpoints","volume":"27","author":"LJ Reed","year":"1938","journal-title":"Am J Hyg"},{"key":"pone.0314516.ref060","volume-title":"Laboratory techniques in rabies","author":"WHO","year":"1996","edition":"4"},{"key":"pone.0314516.ref061","volume-title":"Expression of glycoprotein gene of rabies virus and evaluation of recombinant protein for seromonitoring of vaccinal antibodies in dogs","author":"R. Sharada","year":"2015"},{"key":"pone.0314516.ref062","unstructured":"Anonymous. Bac-to-Bac baculovirus expression system user guide. Invitrogen Life Technologies Corporation; 2015."},{"key":"pone.0314516.ref063","volume-title":"Veterinary Epidemiology","author":"M. Thrusfield","year":"2007"},{"key":"pone.0314516.ref064","volume-title":"Practical statistics for medical research","author":"DG Altman","year":"1991","edition":"1"},{"issue":"2","key":"pone.0314516.ref065","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/0042-6822(92)90465-2","article-title":"Rabies virus glycoprotein is a trimer","volume":"187","author":"Y Gaudin","year":"1992","journal-title":"Virology"},{"issue":"2","key":"pone.0314516.ref066","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1016\/0042-6822(91)90539-N","article-title":"Membrane fusion activity, oligomerization, and assembly of the rabies virus glycoprotein","volume":"185","author":"MA Whitt","year":"1991","journal-title":"Virology"},{"issue":"4","key":"pone.0314516.ref067","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.jbiotec.2008.12.009","article-title":"High-level expression of rabies virus glycoprotein with the RNA-based Semliki Forest Virus expression vector","volume":"139","author":"R Benmaamar","year":"2009","journal-title":"J Biotechnol"},{"issue":"Pt 2","key":"pone.0314516.ref068","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1099\/0022-1317-72-2-359","article-title":"Stable expression of rabies virus glycoprotein in Chinese hamster ovary cells","volume":"72","author":"SR Burger","year":"1991","journal-title":"J Gen Virol"},{"issue":"Pt 4","key":"pone.0314516.ref069","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1099\/0022-1317-64-4-843","article-title":"Antigenic sites on the CVS rabies virus glycoprotein: analysis with monoclonal antibodies","volume":"64","author":"M Lafon","year":"1983","journal-title":"J Gen Virol"},{"issue":"15","key":"pone.0314516.ref070","doi-asserted-by":"crossref","first-page":"10690","DOI":"10.1016\/S0021-9258(19)50073-4","article-title":"N-linked glycosylation of rabies virus glycoprotein. Individual sequons differ in their glycosylation efficiencies and influence on cell surface expression","volume":"267","author":"SH Shakin-Eshleman","year":"1992","journal-title":"J Biol Chem"},{"issue":"8","key":"pone.0314516.ref071","doi-asserted-by":"crossref","first-page":"2599","DOI":"10.1021\/bi00008a026","article-title":"Stable secretion of a soluble, oligomeric form of rabies virus glycoprotein: influence of N-glycan processing on secretion","volume":"34","author":"B Wojczyk","year":"1995","journal-title":"Biochemistry"},{"issue":"2","key":"pone.0314516.ref072","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1093\/glycob\/8.2.121","article-title":"The role of site-specific N-glycosylation in secretion of soluble forms of rabies virus glycoprotein","volume":"8","author":"BS Wojczyk","year":"1998","journal-title":"Glycobiology"},{"issue":"Pt 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Jallet","year":"1999","journal-title":"J Virol"},{"issue":"36","key":"pone.0314516.ref076","doi-asserted-by":"crossref","first-page":"4644","DOI":"10.1016\/j.vaccine.2014.06.058","article-title":"A recombinant rabies vaccine expressing the trimeric form of the glycoprotein confers enhanced immunogenicity and protection in outbred mice","volume":"32","author":"P Koraka","year":"2014","journal-title":"Vaccine"},{"issue":"2","key":"pone.0314516.ref077","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1590\/S0074-02762002000200022","article-title":"Effect of the contents and form of rabies glycoprotein on the potency of rabies vaccination in cattle","volume":"97","author":"AT Piza","year":"2002","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"2","key":"pone.0314516.ref078","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.virusres.2005.05.002","article-title":"DNA vaccine for rabies: relevance of the trans-membrane domain of the glycoprotein in generating an antibody response","volume":"113","author":"A Rath","year":"2005","journal-title":"Virus Res"},{"issue":"1","key":"pone.0314516.ref079","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jbiotec.2005.06.009","article-title":"High level expression of surface glycoprotein of rabies virus in tobacco leaves and its immunoprotective activity in mice","volume":"119","author":"S Ashraf","year":"2005","journal-title":"J Biotechnol"},{"issue":"8","key":"pone.0314516.ref080","doi-asserted-by":"crossref","first-page":"3804","DOI":"10.1128\/jvi.64.8.3804-3809.1990","article-title":"Structural and immunological characterization of a linear virus-neutralizing epitope of the rabies virus glycoprotein and its possible use in a synthetic vaccine","volume":"64","author":"B Dietzschold","year":"1990","journal-title":"J Virol"},{"issue":"2","key":"pone.0314516.ref081","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1006\/prep.1996.0026","article-title":"Purification of a secreted form of recombinant rabies virus glycoprotein: comparison of two affinity tags","volume":"7","author":"BS Wojczyk","year":"1996","journal-title":"Protein Expr Purif"},{"issue":"6","key":"pone.0314516.ref082","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1007\/s10930-012-9420-y","article-title":"Localization of rabies virus glycoprotein into the endoplasmic reticulum produces immunoprotective antigen","volume":"31","author":"DK Yadav","year":"2012","journal-title":"Protein J"},{"issue":"7","key":"pone.0314516.ref083","first-page":"532","article-title":"Enzyme-linked immunosorbent assay (ELISA) using staphylococcal protein A for the measurement of rabies antibody in various species","volume":"36","author":"T Mebatsion","year":"1989","journal-title":"Zentralbl Veterinarmed B"},{"issue":"5","key":"pone.0314516.ref084","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.ijid.2006.09.013","article-title":"Development and evaluation of a competitive ELISA for estimation of rabies neutralizing antibodies after post-exposure rabies vaccination in humans","volume":"11","author":"K Muhamuda","year":"2007","journal-title":"Int J Infect Dis"},{"issue":"8034","key":"pone.0314516.ref085","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/S0140-6736(77)90321-X","article-title":"Enzyme immunoassay for demonstration of rabies-virus antibodies after immunisation","volume":"2","author":"O Thraenhart","year":"1977","journal-title":"Lancet"},{"issue":"Pt 6","key":"pone.0314516.ref086","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1099\/jmm.0.006064-0","article-title":"An evaluation of two commercially available ELISAs and one in-house reference laboratory ELISA for the determination of human anti-rabies virus antibodies","volume":"58","author":"RJ Welch","year":"2009","journal-title":"J Med Microbiol"},{"issue":"4","key":"pone.0314516.ref087","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1017\/S095026880500381X","article-title":"Comparative evaluation of specific ELISA and RFFIT antibody assays in the assessment of dog immunity against rabies","volume":"133","author":"C Bahloul","year":"2005","journal-title":"Epidemiol Infect"},{"issue":"38","key":"pone.0314516.ref088","doi-asserted-by":"crossref","first-page":"5611","DOI":"10.1016\/j.vaccine.2012.06.089","article-title":"Serological methods used for rabies post vaccination surveys: an analysis","volume":"30","author":"P De Benedictis","year":"2012","journal-title":"Vaccine"},{"key":"pone.0314516.ref089","first-page":"40","article-title":"Rapid quantitative assay of rabies post-vaccination antibody by ELISA","volume":"3","author":"RM Karamany","year":"1988","journal-title":"APMIS Suppl"},{"issue":"3","key":"pone.0314516.ref090","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/0092-1157(86)90006-5","article-title":"A rapid rabies enzyme immuno-diagnosis (RREID): a useful and simple technique for the routine diagnosis of rabies","volume":"14","author":"P Perrin","year":"1986","journal-title":"J Biol Stand"},{"issue":"1\u20132","key":"pone.0314516.ref091","first-page":"17","article-title":"Collaborative study to evaluate a new ELISA test to monitor the effectiveness of rabies vaccination in domestic carnivores","volume":"120","author":"Oie Reference Laboratory for Rabies WHO","year":"2006","journal-title":"Virus Res"},{"issue":"6","key":"pone.0314516.ref092","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.biologicals.2016.09.007","article-title":"Rabies vaccine response measurement is assay dependent","volume":"44","author":"SM Moore","year":"2016","journal-title":"Biologicals"},{"key":"pone.0314516.ref093","doi-asserted-by":"crossref","first-page":"101655","DOI":"10.1016\/j.cimid.2021.101655","article-title":"Serosurveillance of rabies antibodies in dogs in Mumbai region by using indirect ELISA","volume":"76","author":"JM Nale","year":"2021","journal-title":"Comp Immunol Microbiol Infect Dis"},{"issue":"18","key":"pone.0314516.ref094","doi-asserted-by":"crossref","first-page":"6547","DOI":"10.1007\/s00253-021-11515-4","article-title":"Detection and quantification of anti-rabies glycoprotein antibodies: current state and perspectives","volume":"105","author":"MC Rodriguez","year":"2021","journal-title":"Appl Microbiol Biotechnol"},{"issue":"3","key":"pone.0314516.ref095","article-title":"Rabies Virus Antibodies from Oral Vaccination as a Correlate of Protection against Lethal Infection in Wildlife","volume":"2","author":"SM Moore","year":"2017","journal-title":"Trop Med Infect 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