{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T03:20:26Z","timestamp":1792466426659,"version":"4.5.0"},"reference-count":61,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Brucellosis and Q-fever are two highly contagious bacterial diseases with significant zoonotic potential and economic threats, yet they often remain underreported and neglected in low- and middle-income countries. The present study aimed to determine the seroprevalence of Brucellosis and Q-fever in water buffaloes in the Haryana state of India to implement effective preventive measures for disease control. The study covered all 22 districts of Haryana and involved 400 serum samples collected from female buffaloes belonging to two age groups and three distinct agro-climatic zones. The collected sera were tested using the Rose Bengal Plate agglutination test (RBPT) and commercial indirect enzyme linked immunosorbent assay (i-ELISA) for the presence of antibodies against smooth strains of <jats:italic>Brucella<\/jats:italic> spp. Additionally, the same serum samples were examined by i-ELISA for antibodies against <jats:italic>Coxiella burnetii<\/jats:italic>. The overall seroprevalence of brucellosis was observed as 8.25% (CI: 5.75\u201311.39) using the RBPT and 7.5% (CI: 5.12\u201310.53) by i-ELISA. The overall observed seroprevalence of Q-fever was 2.00% (CI: 0.87\u20133.90). No significant variation was seen in seropositivity of both diseases based on age and agroclimatic zones of the state. The findings of this study provide critical insights to farmers, agricultural organizations, veterinary services, and healthcare providers, facilitating more effective implementation of disease control measures.<\/jats:p>","DOI":"10.1371\/journal.pone.0314726","type":"journal-article","created":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T18:29:09Z","timestamp":1733423349000},"page":"e0314726","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["Seroprevalance of Brucellosis and Q-fever in water buffaloes (Bubalus bubalis) in Haryana state, India"],"prefix":"10.1371","volume":"19","author":[{"given":"Dharanya","family":"Muthiah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2613-6286","authenticated-orcid":true,"given":"Mahavir","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajesh","family":"Chhabra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renu","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swati","family":"Dahiya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Priya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinu","family":"Manoj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,12,5]]},"reference":[{"key":"pone.0314726.ref001","unstructured":"Livestock Census. Department of Animal Husbandry, Dairying and Fisheries, Ministry of Agriculture, Government of India. 2019; https:\/\/dahd.nic.in\/sites\/default\/filess\/Key%20Results%2BAnnexure%2018.10.2019."},{"key":"pone.0314726.ref002","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1007\/s11250-018-1598-3","article-title":"Seroprevalence of brucellosis and Q fever (Coxiellosis) in cattle herds in Maigana and Birnin Gwari agro-ecological zone of Kaduna State, Nigeria","volume":"50","author":"SG Adamu","year":"2018","journal-title":"Trop Anim Hlth Prod"},{"issue":"1","key":"pone.0314726.ref003","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1080\/01652176.2020.1868616","article-title":"Bovine brucellosis-a comprehensive review","volume":"41","author":"SK Khurana","year":"2021","journal-title":"Vet Q"},{"issue":"2","key":"pone.0314726.ref004","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S1473-3099(06)70382-6","article-title":"The new global map of human brucellosis","volume":"6","author":"G Pappas","year":"2006","journal-title":"Lancet Infect Dis"},{"key":"pone.0314726.ref005","doi-asserted-by":"crossref","first-page":"521","DOI":"10.3389\/fvets.2020.00521","article-title":"One health approach to address zoonotic brucellosis: A spatiotemporal associations study between animals and humans","volume":"7","author":"K Zhou","year":"2020","journal-title":"Front Vet Sci"},{"issue":"3\u20134","key":"pone.0314726.ref006","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.prevetmed.2015.03.013","article-title":"Economic losses occurring due to brucellosis in Indian livestock populations","volume":"119","author":"BB Singh","year":"2015","journal-title":"Prevent Vet Med"},{"key":"pone.0314726.ref007","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1186\/s12889-017-5016-y","article-title":"Brucellosis remains a neglected disease in the developing world: a call for interdisciplinary action","volume":"18","author":"KA Franc","year":"2018","journal-title":"BMC Pub Hlth"},{"key":"pone.0314726.ref008","doi-asserted-by":"crossref","first-page":"208","DOI":"10.4172\/2161-0703.1000208","article-title":"Brucellosis: An economically important infection","volume":"04","author":"Y Bano","year":"2015","journal-title":"J Med Microb Diagn"},{"issue":"3","key":"pone.0314726.ref009","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1111\/tbed.12804","article-title":"Risk factors for occupational Brucella infection in veterinary personnel in India","volume":"65","author":"V Proch","year":"2018","journal-title":"Transbound Emerg Dis"},{"key":"pone.0314726.ref010","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1128\/CMR.00045-16","article-title":"From Q-fever to Coxiella burnetii infection: A paradigm change","volume":"30","author":"C Eldin","year":"2017","journal-title":"Clin Microbiol Rev"},{"key":"pone.0314726.ref011","first-page":"1","article-title":"Review on Q-fever: epidemiology, public health importance and preventive measures","volume":"6","author":"G. Mekonnen","year":"2020","journal-title":"J Biogeneric Sci Res"},{"key":"pone.0314726.ref012","doi-asserted-by":"crossref","first-page":"4271","DOI":"10.3390\/ijerph16214271","article-title":"Serological and molecular investigation of Coxiella burnetii in small ruminants and ticks in Punjab, Pakistan","volume":"16","author":"Q Ullah","year":"2019","journal-title":"Int J Env Res Publ Hlth"},{"key":"pone.0314726.ref013","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.actatropica.2019.03.022","article-title":"Coxiella seroprevalence and risk factors in large ruminants in Bihar and Assam, India","volume":"194","author":"R Shome","year":"2019","journal-title":"Acta Trop"},{"issue":"3","key":"pone.0314726.ref014","doi-asserted-by":"crossref","first-page":"e0264629","DOI":"10.1371\/journal.pone.0264629","article-title":"Q-fever expertise among human and veterinary health professionals in Germany-A stakeholder analysis of knowledge gaps","volume":"17","author":"F Winter","year":"2022","journal-title":"PLOS One"},{"key":"pone.0314726.ref015","unstructured":"World Organization for Animal Health. Brucellosis (infection with Brucella abortus, B. melitensis and B. suis). In: Manual of diagnostic tests and vaccines for terrestrial animals Paris: World Organization for Animal Health (OIE). 2022."},{"key":"pone.0314726.ref016","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/S0378-1135(02)00229-8","article-title":"Diagnosis of brucellosis by serology","volume":"90","author":"K. Nielsen","year":"2002","journal-title":"Vet Microbiol"},{"key":"pone.0314726.ref017","unstructured":"World Organization for Animal Health. Q Fever. In: Manual of diagnostic tests and vaccines for terrestrial animals Paris: World Organization for Animal Health (OIE). 2018."},{"key":"pone.0314726.ref018","unstructured":"National Animal Disease Control Programme (NADCP) [Internet]. Government of India. 2022. https:\/\/dahd.nic.in\/schemes\/programmes\/nadcp."},{"issue":"3","key":"pone.0314726.ref019","article-title":"Performance of agriculture and allied sectors in Haryana","volume":"5","author":"A. Sharma","year":"2014","journal-title":"J Econom Sustain Dev"},{"key":"pone.0314726.ref020","unstructured":"Thrusfield M. Veterinary Epidemiology, 2nd ed. CRC. Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies. University of Edinburg. 1995."},{"issue":"3\u20134","key":"pone.0314726.ref021","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.anireprosci.2010.08.022","article-title":"Reproductive cycles of buffalo","volume":"124","author":"BM Perera","year":"2011","journal-title":"Anim Reprod Sci"},{"issue":"5","key":"pone.0314726.ref022","first-page":"1395","article-title":"One health approach: An overview of Q-fever in livestock, wildlife and humans in Asturias (Northwestern Spain)","volume":"11","author":"A Espi","year":"2021","journal-title":"Anim (Basel)"},{"key":"pone.0314726.ref023","doi-asserted-by":"crossref","first-page":"2741","DOI":"10.2147\/IDR.S319595","article-title":"Epidemiological, clinical and laboratory characteristics of patients with Brucella infection in Anhui Province, China","volume":"14","author":"C Shi","year":"2021","journal-title":"Infect Drug 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Kaynak-Onurdag","year":"2016","journal-title":"J Dairy Sci"},{"key":"pone.0314726.ref027","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.3390\/ijerph17041412","article-title":"Serological and molecular detection of bovine brucellosis at institutional livestock farms in Punjab, Pakistan","volume":"17","author":"T Jamil","year":"2020","journal-title":"Int J Env Res Publ Hlth"},{"key":"pone.0314726.ref028","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S0378-1135(02)00253-5","article-title":"Epidemiology, zoonotic aspects, vaccination and control\/eradication of brucellosis in India","volume":"90","author":"GJ Renukaradhya","year":"2002","journal-title":"Vet Microbiol"},{"issue":"1","key":"pone.0314726.ref029","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/S0255-0857(21)02230-1","article-title":"Brucellosis in high risk group individuals","volume":"25","author":"A Agasthya","year":"2007","journal-title":"Indian J Med Microbiol"},{"issue":"2","key":"pone.0314726.ref030","first-page":"61","article-title":"Comparison of Rose Bengal plate agglutination, standard tube agglutination and indirect ELISA tests for detection of Brucella antibodies in cows and buffaloes","volume":"3","author":"SN Ghodasara","year":"2010","journal-title":"Vet World"},{"issue":"8","key":"pone.0314726.ref031","doi-asserted-by":"crossref","first-page":"559","DOI":"10.20546\/ijcmas.2019.808.067","article-title":"Serosurveillance of bovine brucellosis using RBPT and c-ELISA and comparative evaluation of test performance","volume":"8","author":"MU Yuguda","year":"2019","journal-title":"Int J Curr Microbiol App Sci"},{"issue":"7","key":"pone.0314726.ref032","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.20546\/ijcmas.2020.907.226","article-title":"Brucellosis and associated risk factors analysis in serum sample of bovines","volume":"9","author":"RP Mishra","year":"2020","journal-title":"Int J Curr Microbiol App 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Hlth"},{"key":"pone.0314726.ref043","doi-asserted-by":"crossref","first-page":"244","DOI":"10.5455\/ijlr.20170915054130","article-title":"Seroprevalence of brucellosis in buffaloes by indirect enzyme linked immune-sorbent assay in Punjab, India","volume":"8","author":"MR Islam","year":"2018","journal-title":"Int J Livestock Res"},{"issue":"6","key":"pone.0314726.ref044","doi-asserted-by":"crossref","first-page":"3303","DOI":"10.20546\/ijcmas.2020.906.393","article-title":"Seroprevalence of brucellosis in large and small ruminants in Chhattisgarh state","volume":"9","author":"S Shakya","year":"2020","journal-title":"India. Int J Curr Microbiol App Sci"},{"key":"pone.0314726.ref045","first-page":"1556548","article-title":"Bovine brucellosis: prevalence, risk factors, economic cost and control options with particular reference to India\u2014a review","volume":"8","author":"RP Deka","year":"2018","journal-title":"Infect Ecol Epidemiol"},{"key":"pone.0314726.ref046","first-page":"1","volume":"43","author":"PA Jaismon","year":"2023","journal-title":"Prevalence of bovine brucellosis in India: a meta analysis"},{"issue":"1","key":"pone.0314726.ref047","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/s13104-017-2394-2","article-title":"Seroprevalence and risk factors associated with bovine brucellosis in the Potohar Plateau, Pakistan","volume":"10","author":"S Ali","year":"2017","journal-title":"BMC Res. Notes"},{"key":"pone.0314726.ref048","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1111\/j.1865-1682.2010.01171.x","article-title":"Serological evidence of Brucella abortus prevalence in Punjab province, Pakistan\u2014a cross-sectional study","volume":"57","author":"M Abubakar","year":"2010","journal-title":"Transbound Emerg Dis"},{"issue":"9","key":"pone.0314726.ref049","doi-asserted-by":"crossref","first-page":"e0239260","DOI":"10.1371\/journal.pone.0239260","article-title":"Apparent prevalence and risk factors of coxiellosis (Q fever) among dairy herds in India","volume":"15","author":"P Dhaka","year":"2020","journal-title":"PLOS One"},{"issue":"1","key":"pone.0314726.ref050","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1186\/1297-9716-44-67","article-title":"Q-fever in pregnant goats: humoral and cellular immune responses","volume":"44","author":"HIJ Roest","year":"2013","journal-title":"Vet 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