{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T15:16:23Z","timestamp":1793978183070,"version":"4.8.0"},"reference-count":39,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T00:00:00Z","timestamp":1749772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004508","name":"Prince of Songkla University","doi-asserted-by":"publisher","award":["VET6604128S"],"award-info":[{"award-number":["VET6604128S"]}],"id":[{"id":"10.13039\/501100004508","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    This study aimed to characterize zoonotic yeasts from zebra dove (\n                    <jats:italic>Geopelia striata<\/jats:italic>\n                    ) droppings in small farms in Songkhla province, Thailand. Four out of thirty-one isolates were found with morphology and biochemical test results like those of\n                    <jats:italic>Cryptococcus<\/jats:italic>\n                    spp. (12.9%) but they exhibited different results from the positive control in nested polymerase chain reaction (PCR); the first step with ITS1-ITS4 primers was negative, but the nested PCR step with CN4-CN5 was positive. All isolates were subjected to antifungal susceptibility testing. Only the Tip11 isolate showed high resistance to itraconazole and ketoconazole, with minimum inhibitory concentrations of &gt;32.0 and 32.0 \u00b5g\/mL, respectively, corresponding to high levels of minimum fungicidal concentrations for both drugs (&gt;32 \u00b5g\/mL). Consequently, Tip11 was selected as a representative of the contaminated isolates and subjected to whole-genome sequencing analysis. The results identified the Tip11 isolate as\n                    <jats:italic>Hyphopichia burtonii<\/jats:italic>\n                    (99.30% identity), with a genome length of 12,360,159 bp, a GC content of 35.16%, and 2,146 protein groups closely related to the\n                    <jats:italic>Saccharomycetaceae<\/jats:italic>\n                    family. It showed 43 candidates resistance genes of antifungal drugs mostly in azole and itraconazole group which related to the antifungal susceptibility testing. This is the first report of\n                    <jats:italic>H. burtonii<\/jats:italic>\n                    in zebra doves in Thailand. This yeast has been previously identified as a cause of human infection leading to peritonitis in Thailand, and its resistance to antifungal drugs may pose a public health risk. Therefore, the application of biosecurity measures on farms, such as regular removal of droppings and cage sanitization, should be implemented according to good agricultural practices.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0315067","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T13:37:26Z","timestamp":1749821846000},"page":"e0315067","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Whole-genome sequencing of Hyphopichia burtonii from isolated yeast recovered from zebra dove droppings in Thailand"],"prefix":"10.1371","volume":"20","author":[{"given":"Saowakon","family":"Indoung","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2981-8526","authenticated-orcid":true,"given":"Sanicha","family":"Chumtong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakaoporn","family":"Prachantasena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ratchakul","family":"Wiriyaprom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7793-7561","authenticated-orcid":true,"given":"Komwit","family":"Surachat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rattanaruji","family":"Pomwised","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0000-6018","authenticated-orcid":true,"given":"Ruttayaporn","family":"Ngasaman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,6,13]]},"reference":[{"key":"pone.0315067.ref001","year":"2017"},{"issue":"1","key":"pone.0315067.ref002","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/1297-9716-44-36","article-title":"Zoonoses 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