{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T12:19:44Z","timestamp":1785932384603,"version":"3.56.0"},"reference-count":98,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2024,12,6]],"date-time":"2024-12-06T00:00:00Z","timestamp":1733443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["320450"],"award-info":[{"award-number":["320450"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>In recent years, different laboratories have provided evidence on the role of miRNAs in regulation of corneal epithelial metabolism, permeability and wound healing, as well as their alteration after surgery and in some ocular pathologies. We searched the available databases reporting miRNA expression in the human eye, looking for miRNAs highly expressed in central cornea, which could be crucial for maintenance of the epithelial phenotype. Using the rabbit RCE1(5T5) cell line as a model of corneal epithelial differentiation, we describe the participation of miR-141-3p as a possible negative regulator of the proliferative\/migratory phenotype in corneal epithelial cells. The expression of miR-141-3p followed a time course similar to the differentiation-linked KRT3 cytokeratin, being delayed 24\u201348 hours relative to PAX6 expression; such result suggested that miR-141-3p only regulates the expression of terminal phenotype. Inhibition of miR-141-3p led to increased cell proliferation and motility, and induced the expression of molecular makers characteristic of an Epithelial Mesenchymal Transition (EMT). Comparison between the transcriptional profile of cells in which miR-141-3p was knocked down, and the transcriptomes from proliferative non-differentiated and differentiated stratified epithelia suggest that miR-141-3p is involved in the expression of terminal differentiation mediating the arrest of cell proliferation and inhibiting the EMT in highly motile early differentiating cells.<\/jats:p>","DOI":"10.1371\/journal.pone.0315296","type":"journal-article","created":{"date-parts":[[2024,12,6]],"date-time":"2024-12-06T18:29:59Z","timestamp":1733509799000},"page":"e0315296","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Regulation of corneal epithelial differentiation: miR-141-3p promotes the arrest of cell proliferation and enhances the expression of terminal phenotype"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4297-9598","authenticated-orcid":true,"given":"Mar\u00eda Teresa","family":"Ortiz-Melo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge E.","family":"Campos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"S\u00e1nchez-Guzm\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Esther","family":"Herrera-Aguirre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6290-6017","authenticated-orcid":true,"given":"Federico","family":"Castro-Mu\u00f1ozledo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,12,6]]},"reference":[{"key":"pone.0315296.ref001","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.semcdb.2014.06.005","article-title":"Hox gene regulation and timing in embryogenesis","volume":"34","author":"T Montavon","year":"2014","journal-title":"Semin Cell Dev Biol"},{"key":"pone.0315296.ref002","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1111\/cge.12493","article-title":"Genetic regulation of vertebrate eye development","volume":"86","author":"JL Zagozewski","year":"2014","journal-title":"Clin Genet"},{"issue":"1\u20132","key":"pone.0315296.ref003","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0378-1119(00)00454-6","article-title":"Regulatory roles of AP-2 transcription factors in vertebrate development, apoptosis, and cell-cycle control.","volume":"260","author":"K Hilger-Eversheim","year":"2000","journal-title":"Gene"},{"issue":"12","key":"pone.0315296.ref004","doi-asserted-by":"crossref","first-page":"4283","DOI":"10.3390\/ijms21124283","article-title":"Adipogenesis: A Complex Interplay of Multiple Molecular Determinants and Pathways.","volume":"21","author":"MA Ambele","year":"2020","journal-title":"Int J Mol Sci"},{"issue":"2","key":"pone.0315296.ref005","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.ydbio.2014.07.004","article-title":"The role of homeobox genes in retinal development and disease","volume":"393","author":"JL Zagozewski","year":"2014","journal-title":"Dev Biol"},{"key":"pone.0315296.ref006","doi-asserted-by":"crossref","first-page":"4092","DOI":"10.1167\/iovs.05-0324","article-title":"Developmentally regulated expression of Sp1 in the mouse cornea","volume":"46","author":"H Nakamura","year":"2005","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"pone.0315296.ref007","doi-asserted-by":"crossref","first-page":"3109","DOI":"10.1167\/iovs.05-0053","article-title":"Involucrin expression in the corneal epithelium: an essential role for Sp1 transcription factors","volume":"46","author":"G Adhikary","year":"2005","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"pone.0315296.ref008","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/bs.pmbts.2015.04.003","article-title":"Corneal Development: Different Cells from a Common Progenitor.","volume":"134","author":"P.Y. 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