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The aim of this study is to compare different methods of normalizing the sEMG signal of respiratory muscles in both healthy subjects and those with Amyotrophic Lateral Sclerosis (ALS). This cross-sectional study was conducted in 67 subjects (50 healthy and 17 with ALS). The electrical activity of the sternocleidomastoid (SCM), scalene (ESC), diaphragm (DIA), parasternal (PS), external intercostal (EI), external oblique (EO), and rectus abdominal (RA) muscles were analyzed during maximal inspiratory pressure maneuvers (MIP), maximal nasal inspiratory pressure (SNIP), maximal expiratory pressure (MEP), and maximal voluntary isometric contraction of SCM and ESC (MVIC<jats:sub>SCM\/ESC<\/jats:sub>) and RA (MVIC<jats:sub>RA<\/jats:sub>) using surface electromyography (sEMG). In the healthy group, inspiratory and expiratory muscles displayed higher electrical activity during MVIC<jats:sub>SCM\/ESC<\/jats:sub> and MIVC<jats:sub>RA<\/jats:sub> maneuvers, respectively (p&lt;0.05). In the ALS group, inspiratory muscle activity was higher during the SNIP maneuver, while expiratory muscles showed higher activity during MVICRA (p&lt;0.05). Based on the findings, it can be concluded that the MVIC resulted in greater inspiratory muscle activity, being the ideal method of normalization for inspiratory and expiratory muscles in healthy subjects. In ALS patients, the SNIP maneuver resulted in greater inspiratory muscle activity, while MVIC resulted in greater muscle activity in expiratory muscles.<\/jats:p>","DOI":"10.1371\/journal.pone.0315846","type":"journal-article","created":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T18:26:38Z","timestamp":1734719198000},"page":"e0315846","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Methods to normalize surface electromyography in respiratory muscles: Is it similar between amyotrophic lateral sclerosis and healthy people?"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7364-4016","authenticated-orcid":true,"given":"Thiago Bezerra Wanderley e","family":"Lima","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1782-2217","authenticated-orcid":true,"given":"Jessica Danielle Medeiros da","family":"Fonseca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Aline Marcelino da","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rayane Grayce da Silva","family":"Vieira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dayane","family":"Montemezzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matias","family":"Otto-Y\u00e1\u00f1ez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Torres-Castro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rio Em\u00edlio Teixeira Dourado","family":"J\u00fanior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa Regiane","family":"Resqueti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4938-7018","authenticated-orcid":true,"given":"Guilherme Augusto de Freitas","family":"Fregonezi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,12,20]]},"reference":[{"issue":"3","key":"pone.0315846.ref001","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1080\/10803548.2017.1314120","article-title":"Repeatability of electromyography normalization of the neck and shoulder muscles in symptomatic office workers","volume":"24","author":"M Chaikumarn","year":"2018","journal-title":"Int J Occup Saf Ergon"},{"issue":"2","key":"pone.0315846.ref002","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.jelekin.2007.07.007","article-title":"The effects of interelectrode distance over the innervation zone and normalization on the electromyographic amplitude and mean power frequency versus concentric, eccentric, and isometric torque relationships for the vastus lateralis muscle","volume":"19","author":"TW Beck","year":"2009","journal-title":"J Electromyogr Kinesiol"},{"issue":"5","key":"pone.0315846.ref003","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.1152\/japplphysiol.00914.2003","article-title":"Reproducibility and responsiveness of a noninvasive EMG technique of the respiratory muscles in COPD patients and in healthy subjects","volume":"96","author":"ML Duiverman","year":"2004","journal-title":"J Appl Physiol (1985)"},{"issue":"6","key":"pone.0315846.ref004","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1016\/j.jelekin.2010.07.004","article-title":"How should we normalize electromyograms obtained from healthy participants? 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