{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T21:22:05Z","timestamp":1792012925362,"version":"4.4.0"},"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T00:00:00Z","timestamp":1739836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Basic Research Project of Wangjing Hospital, China Academy of Chinese Medical Sciences","award":["WJYY-YJKT-2022-04"],"award-info":[{"award-number":["WJYY-YJKT-2022-04"]}]},{"name":"Science and Technology Innovation Project of China Academy of Chinese Medical Sciences","award":["CI2021A04901"],"award-info":[{"award-number":["CI2021A04901"]}]},{"name":"Science and Technology Innovation Project of China Academy of Chinese Medical Sciences","award":["CI2021A05054"],"award-info":[{"award-number":["CI2021A05054"]}]},{"name":"the Fundamental Research Funds for the Central public welfare research institutes","award":["ZZ13-YQ-036"],"award-info":[{"award-number":["ZZ13-YQ-036"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["No.82174415"],"award-info":[{"award-number":["No.82174415"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Shaoyao Gancao Decoction (SGD) is a classic and representative oral administration of traditional Chinese medicine formula. It is composed of two Chinese herbal medicines, <jats:italic>Paeoniae Radix Alba [Paeonia lactiflora Pall]<\/jats:italic> and <jats:italic>Glycyrrhizae Radix et Rhizoma<\/jats:italic>. The clinical study found SGD could effectively reduce clinical symptoms and improve the level of inflammation in osteoarthritis (OA) patients.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Purpose<\/jats:title>\n<jats:p>The aim of this study is to identify the efficacy and molecular mechanism of SGD in the treatment of OA, and find the new therapeutic target through RNA sequencing (RNA-Seq) to provide theoretical support for its clinical application.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Methods<\/jats:title>\n<jats:p>Destabilization of the medial meniscus (DMM) OA rat model was established in vivo. Hematoxylineosin staining, safranin O\/fast green staining and immunohistochemistry were used to observe changes of cartilage Histology and extracellular matrix (ECM) of cartilage cells. In vitro, the chondrocyte-like cells were derived from ATDC5 cells and induced by interleukin-1 beta to establish the model. The medial meniscotibial ligament (MTT) test was used to identify the effects of SGD on chondrocyte-like cell proliferation, and immunocytochemistry was used to assess changes in chondrocyte ECM. The differentially expressed genes (DEGs) were obtained by RNA-Seq. Meanwhile, the core targets were found through bioinformatics analysis, and then verified by qRT-PCR and Western Blotting. The inflammatory factors IL-1\u03b2, IL-6 and TNF-\u03b1 were detected by ELISA.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Results<\/jats:title>\n<jats:p>SGD could alleviate cartilage degeneration, and reduce ECM degradation in OA by upregulating COL2A1 and downregulating MMP-13. 120 key targets were screened from DEGs by RNA-Seq. Based on further bioinformatics analysis, interleukin 17 receptor B (IL-17RB), interleukin 23 receptor and growth differentiation factor 5 were finally selected as core targets. IL-17RB has rarely been reported in previous studies about OA, and worthy of further study. Subsequently, it was found that the gene and protein expressions of IL-17RB were significantly reversed in model group after SGD treatment. Moreover, SGD could inhibit the release of inflammatory factors by mediating IL-17RB in OA.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec005\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>SGD reduced the release of inflammatory factors IL-1\u03b2, IL-6 and TNF-\u03b1, upregulated COL2A1 and downregulated MMP-13 to alleviate degradation of ECM, and reduced the cartilage degeneration and progression of OA by reducing IL-17RB in articular cartilage.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0315913","type":"journal-article","created":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T18:27:51Z","timestamp":1739903271000},"page":"e0315913","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Identification of the key role of IL-17RB in the treatment of osteoarthritis with Shaoyao Gancao decoction: Verification based on RNA-seq and bioinformatics analysis"],"prefix":"10.1371","volume":"20","author":[{"given":"Chengzhi","family":"Hou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5259-9018","authenticated-orcid":true,"given":"Zhangjingze","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghui","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuelei","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangcheng","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuyue","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,2,18]]},"reference":[{"issue":"5","key":"pone.0315913.ref001","first-page":"3","article-title":"Osteoarthritis is a serious disease","volume":"120","author":"G.A. 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Na","year":"2020","journal-title":"Front Immunol"},{"key":"pone.0315913.ref039","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.molimm.2017.07.004","article-title":"IL-17B: A new area of study in the IL-17 family","volume":"90","author":"Q. Bie","year":"2017","journal-title":"Mol Immunol"},{"issue":"1","key":"pone.0315913.ref040","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1093\/rheumatology\/ket309","article-title":"Neutrophils produce interleukin-17B in rheumatoid synovial tissue","volume":"53","author":"V.P. Kouri","year":"2014","journal-title":"Rheumatology (Oxford)"},{"issue":"1","key":"pone.0315913.ref041","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/1348-0421.12560","article-title":"Interleukin-17 family cytokines in protective immunity against infections: role of hematopoietic cell-derived and non-hematopoietic cell-derived interleukin-17s","volume":"62","author":"G. 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