{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T11:53:15Z","timestamp":1785498795026,"version":"3.56.0"},"reference-count":83,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T00:00:00Z","timestamp":1738195200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Natural Science Foundation Project of Heilongjiang Province","award":["LH2021H112"],"award-info":[{"award-number":["LH2021H112"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82271070"],"award-info":[{"award-number":["82271070"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Heilongjiang Provincial Undergraduate Colleges and Universities Central to Support the Reform and Development of Local Colleges and Universities","award":["2020YQ08"],"award-info":[{"award-number":["2020YQ08"]}]},{"name":"Mudanjiang Medical University Affiliated Hongqi Hospital","award":["2024-HQBS-03"],"award-info":[{"award-number":["2024-HQBS-03"]}]},{"name":"the Nonprofit Central Research Institute Fund of National Research Institute for Family Planning","award":["2022GJZ02"],"award-info":[{"award-number":["2022GJZ02"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>DNA methylation is known to be associated with cataracts. In this study, we used a mouse model and performed DNA methylation and transcriptome sequencing analyses to find epigenetic indicators for age-related cataracts (ARC). Anterior lens capsule membrane tissues from young and aged mice were analyzed by MethylRAD-seq to detect the genome-wide methylation of extracted DNA. The young and aged mice had 76,524 and 15,608 differentially methylated CCGG and CCWGG sites, respectively. The Pearson correlation analysis detected 109 and 33 differentially expressed genes (DEGs) with negative methylation at CCGG and CCWGG sites, respectively, in their promoter regions. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses showed that DEGs with abnormal methylation at CCGG sites were primarily associated with protein kinase C signaling <jats:italic>(Akap12<\/jats:italic>, <jats:italic>Capzb<\/jats:italic>), protein threonine kinase activity (<jats:italic>Dmpk<\/jats:italic>, <jats:italic>Mapkapk3<\/jats:italic>), and calcium signaling pathway (<jats:italic>Slc25a4<\/jats:italic>, <jats:italic>Cacna1f<\/jats:italic>), whereas DEGs with abnormal methylation at CCWGG sites were associated with ribosomal protein S6 kinase activity (<jats:italic>Rps6ka3<\/jats:italic>). These genes were validated by pyrosequencing methylation analysis. The results showed that the ARC group (aged mice) had lower <jats:italic>Dmpk<\/jats:italic> and <jats:italic>Slc25a4<\/jats:italic> methylation levels and a higher <jats:italic>Rps6ka3<\/jats:italic> methylation than the control group (young mice), which is consistent with the results of the joint analysis of differentially methylated and differentially expressed genes. In conclusion, we confirmed the genome-wide DNA methylation pattern and gene expression profile of ARC based on the mouse cataract model with aged mice. The identified methylation molecular markers have great potential for application in the future diagnosis and treatment of ARC.<\/jats:p>","DOI":"10.1371\/journal.pone.0316766","type":"journal-article","created":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T18:43:52Z","timestamp":1738262632000},"page":"e0316766","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genome-wide DNA methylation and transcriptome sequencing analyses of lens tissue in an age-related mouse cataract 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