{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:02:45Z","timestamp":1787022165125,"version":"build-2736575974"},"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T00:00:00Z","timestamp":1740614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100015717","name":"HEAD-Genuit-Stiftung","doi-asserted-by":"publisher","award":["HGS03S18072016"],"award-info":[{"award-number":["HGS03S18072016"]}],"id":[{"id":"10.13039\/501100015717","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Significant autonomic nervous system responses to a specific osteopathic intervention, the cranial vault hold (CVH), have recently been demonstrated in forehead skin blood volume changes, heart rate, and respiration frequencies. The specificity of the CVH-intervention-related autonomic responses yet requires differentiation. Thus, we compared autonomic responses to CVH with responses to compression of the fourth ventricle (CV4) and to two corresponding SHAM conditions. Analysis of frequencies and amplitudes for changes in skin blood volume and respiration in low (LF; 0.05\u20130.12 Hz), intermediate (IM; 0.12\u20130.18 Hz), and high (HF; 0.18\u20130.4 Hz) frequency bands, and metrics of heartrate variability revealed significant decreases in LF range (from 0.12 to 0.10 Hz), increased LF and decreased IM durations, and increased skin blood volume amplitudes in response to CVH, but no significant skin blood volume responses to any of the control interventions. Ratio changes for respiration and skin blood volume frequencies approximately at 3:1 during CVH, remained unchanged in all other interventions. Heart rate decreased across conditions, indicating an increase in parasympathetic tone. This was also indicated by a significant increase in root mean of squared successive difference following CV4. We incurred that rhythmic response patterns in the LF and IM bands only appeared in CVH. This suggests specific physiological responses to CVH warranting further investigation by studying e.g., responses to CVH in physical or mental health disorders with autonomic involvement.<\/jats:p>","DOI":"10.1371\/journal.pone.0317300","type":"journal-article","created":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T13:23:08Z","timestamp":1740662588000},"page":"e0317300","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Specificity of cranial cutaneous manipulations in modulating autonomic nervous system responses and physiological oscillations: A controlled study"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5221-4890","authenticated-orcid":true,"given":"Micha","family":"Keller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"Perlitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger","family":"Pelz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Borik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ines","family":"Repik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armin","family":"Geilgens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7623-2279","authenticated-orcid":true,"given":"Birol","family":"Cotuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gero","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Mathiak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Mayer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,2,27]]},"reference":[{"issue":"1","key":"pone.0317300.ref001","article-title":"Entrainment and the cranial rhythmic impulse","volume":"3","author":"JM McPartland","year":"1997","journal-title":"Altern Ther Health Med"},{"key":"pone.0317300.ref002","doi-asserted-by":"crossref","first-page":"197","DOI":"10.24966\/ACIM-7562\/100197","article-title":"Osteopathy in the cranial field from a systems theory perspective","volume":"7","author":"RJ Zweedijk","year":"2021","journal-title":"J Altern Complement Integr Med"},{"issue":"12","key":"pone.0317300.ref003","doi-asserted-by":"crossref","first-page":"e0167823","DOI":"10.1371\/journal.pone.0167823","article-title":"Reliability of diagnosis and clinical efficacy of cranial osteopathy: a systematic review","volume":"11","author":"A Guillaud","year":"2016","journal-title":"PLoS One"},{"issue":"6","key":"pone.0317300.ref004","article-title":"Recording the rate of the cranial rhythmic impulse","volume":"106","author":"KE Nelson","year":"2006","journal-title":"Journal of the American Osteopathic Association"},{"key":"pone.0317300.ref005","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijosm.2020.01.003","article-title":"Models and theoretical frameworks for osteopathic care\u2013a critical view and call for updates and research","volume":"35","author":"JE Esteves","year":"2020","journal-title":"International Journal of Osteopathic Medicine"},{"key":"pone.0317300.ref006","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.ijosm.2018.10.001","article-title":"Reflecting on new models for osteopathy\u2013it\u2019s time for change","volume":"31","author":"D. Smith","year":"2019","journal-title":"International Journal of Osteopathic Medicine"},{"key":"pone.0317300.ref007","doi-asserted-by":"crossref","first-page":"887","DOI":"10.3389\/fnins.2020.00887","article-title":"Does osteopathic manipulative treatment induce autonomic changes in healthy participants? A thermal imaging study","author":"F Cerritelli","year":"2020","journal-title":"Front Neurosci"},{"issue":"2\u20133","key":"pone.0317300.ref008","article-title":"Phase transitions in the common brainstem and related systems investigated by nonstationary time series analysis","volume":"78","author":"M Lambertz","year":"2000","journal-title":"J Auton Nerv Syst"},{"issue":"6","key":"pone.0317300.ref009","doi-asserted-by":"crossref","DOI":"10.1007\/s00424-004-1291-4","article-title":"Cardiovascular rhythms in the 0.15-Hz band: Common origin of identical phenomena in man and dog in the reticular formation of the brain stem?","volume":"448","author":"V Perlitz","year":"2004","journal-title":"Pflugers Arch"},{"key":"pone.0317300.ref010","article-title":"Dynamik des 0,15-Hz Rhythmusbandes in der Hautdurchblutung bei Musikexposition","author":"JP Ernst","year":"2012","journal-title":"Hochschulbibliothek der Rheinisch Westf\u00e4lischen Technischen Hochschule Aachen"},{"issue":"3","key":"pone.0317300.ref011","doi-asserted-by":"crossref","DOI":"10.1016\/S1615-9071(15)30036-8","article-title":"Inh\u00e4rente Rhythmen\u2014Komplexe psychophysische Synergismen durch Synchronisation","volume":"16","author":"H. Pelz","year":"2015","journal-title":"Osteopathische Medizin"},{"issue":"9","key":"pone.0317300.ref012","doi-asserted-by":"crossref","DOI":"10.1111\/psyp.13594","article-title":"Neural correlates of fluctuations in the intermediate band for heart rate and respiration are related to interoceptive perception","volume":"57","author":"M Keller","year":"2020","journal-title":"Psychophysiology"},{"key":"pone.0317300.ref013","article-title":"Low-frequency facial hemodynamic oscillations distinguish migraineurs from non-headache controls","volume":"2","author":"MM Cortez","year":"2019","journal-title":"Cephalalgia Rep"},{"issue":"3","key":"pone.0317300.ref014","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1016\/j.clinph.2019.11.013","article-title":"Heart rate variability (HRV): From brain death to resonance breathing at 6 breaths per minute","volume":"131","author":"AR Schwerdtfeger","year":"2020","journal-title":"Clinical Neurophysiology"},{"key":"pone.0317300.ref015","doi-asserted-by":"crossref","first-page":"113676","DOI":"10.1016\/j.physbeh.2021.113676","article-title":"Negative respiratory sinus arrhythmia (nRSA) in the MRI-scanner-a physiologic phenomenon observed during elevated anxiety in healthy persons","volume":"245","author":"B Rassler","year":"2022","journal-title":"Physiol Behav"},{"issue":"1","key":"pone.0317300.ref016","doi-asserted-by":"crossref","first-page":"e15891","DOI":"10.14814\/phy2.15891","article-title":"Autonomic nervous system responses in the intermediate band to cranial cutaneous stimulation","volume":"12","author":"M Keller","year":"2024","journal-title":"Physiol Rep"},{"key":"pone.0317300.ref017","article-title":"Cranial Manipulation Induces Sequential Changes in Blood Flow Velociy on Demand","author":"K.E. Nelson","year":"2004","journal-title":"The AAO Journal"},{"issue":"12","key":"pone.0317300.ref018","first-page":"660","article-title":"Effect of cranial osteopathic manipulative medicine on cerebral tissue oxygenation","volume":"111","author":"X Shi","year":"2011","journal-title":"Journal of Osteopathic Medicine"},{"key":"pone.0317300.ref019","first-page":"10","article-title":"Effect of manual approaches with osteopathic modality on brain correlates of interoception: an fMRI study","author":"F Cerritelli","year":"2020","journal-title":"Sci Rep"},{"issue":"1","key":"pone.0317300.ref020","first-page":"22","article-title":"Das ICD-10-symptom-rating (ISR): Zusammenh\u00e4nge mit der SCL-90-R","volume":"3","author":"K Tritt","year":"2010","journal-title":"Klinische Diagnostik und Evaluation"},{"issue":"1","key":"pone.0317300.ref021","doi-asserted-by":"crossref","first-page":"6611","DOI":"10.1038\/s41598-023-33644-8","article-title":"Validation of subjective manual palpation using objective physiological recordings of the cranial rhythmic impulse during osteopathic manipulative intervention","volume":"13","author":"H Pelz","year":"2023","journal-title":"Sci Rep"},{"issue":"1","key":"pone.0317300.ref022","doi-asserted-by":"crossref","first-page":"5997","DOI":"10.1038\/s41598-022-09762-0","article-title":"On the spatial phase distribution of cutaneous low-frequency perfusion oscillations","volume":"12","author":"S Borik","year":"2022","journal-title":"Sci Rep"},{"issue":"7825","key":"pone.0317300.ref023","doi-asserted-by":"crossref","DOI":"10.1038\/s41586-020-2649-2","article-title":"Array programming with NumPy","volume":"585","author":"CR Harris","year":"2020","journal-title":"Nature"},{"issue":"3","key":"pone.0317300.ref024","article-title":"SciPy 1.0: fundamental algorithms for scientific computing in Python","volume":"17","author":"P Virtanen","year":"2020","journal-title":"Nat Methods"},{"issue":"3","key":"pone.0317300.ref025","doi-asserted-by":"crossref","DOI":"10.1109\/MCSE.2007.55","article-title":"Matplotlib: A 2D graphics environment","volume":"9","author":"JD Hunter","year":"2007","journal-title":"Comput Sci Eng"},{"key":"pone.0317300.ref026","doi-asserted-by":"crossref","first-page":"258","DOI":"10.3389\/fpubh.2017.00258","article-title":"An overview of heart rate variability metrics and norms","author":"F Shaffer","year":"2017","journal-title":"Front Public Health"},{"issue":"4","key":"pone.0317300.ref027","doi-asserted-by":"crossref","DOI":"10.1016\/j.ostmed.2011.10.010","article-title":"Kranialer rhythmischer Impuls: Ist die palpierte Frequenz abh\u00e4ngig von der Berufserfahrung der Untersucher?","volume":"12","author":"N Sergueef","year":"2011","journal-title":"Osteopathische Medizin"},{"issue":"6","key":"pone.0317300.ref028","article-title":"Synergetik der hypnoiden Relaxation","volume":"54","author":"V Perlitz","year":"2004","journal-title":"PPmP Psychotherapie Psychosomatik Medizinische Psychologie"},{"issue":"12","key":"pone.0317300.ref029","doi-asserted-by":"crossref","first-page":"e650","DOI":"10.1016\/S2589-7500(20)30246-6","article-title":"Heart rate variability with photoplethysmography in 8 million individuals: a cross-sectional study","volume":"2","author":"A Natarajan","year":"2020","journal-title":"Lancet Digit Health"},{"issue":"1\u20132","key":"pone.0317300.ref030","article-title":"Coordination dynamics of circulatory and respiratory rhythms during psychomotor drive reduction","volume":"115","author":"V Perlitz","year":"2004","journal-title":"Auton Neurosci"},{"issue":"1","key":"pone.0317300.ref031","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40001-019-0394-5","article-title":"Effectiveness of an osteopathic treatment on the autonomic nervous system: a systematic review of the literature","volume":"24","author":"V Rechberger","year":"2019","journal-title":"Eur J Med Res"},{"key":"pone.0317300.ref032","first-page":"14","article-title":"Verification of a Central Pacemaker in Brain Stem by Phase-Coupling Analysis Between HR Interval- and BOLD-Oscillations in the 0.10\u20130.15 Hz Frequency Band","author":"G Pfurtscheller","year":"2020","journal-title":"Front Neurosci"},{"issue":"3","key":"pone.0317300.ref033","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1111\/j.1475-097X.1996.tb00578.x","article-title":"Electrodermal activity reveals respiratory and slower rhythms of the autonomic nervous system","volume":"16","author":"J Rittweger","year":"1996","journal-title":"Clinical Physiology"},{"issue":"6","key":"pone.0317300.ref034","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1046\/j.1365-2281.1997.00058.x","article-title":"Influences of mandatory breathing on rhythmical components of electrodermal activity","volume":"17","author":"J Rittweger","year":"1997","journal-title":"Clinical physiology"},{"issue":"7","key":"pone.0317300.ref035","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1002\/ca.23391","article-title":"Active contractile properties of fascia","volume":"32","author":"R Schleip","year":"2019","journal-title":"Clinical Anatomy"},{"issue":"20","key":"pone.0317300.ref036","doi-asserted-by":"crossref","first-page":"6220","DOI":"10.3390\/jcm11206220","article-title":"Cardiac-Gated Neuromodulation Increased Baroreflex Sensitivity and Reduced Pain Sensitivity in Female Fibromyalgia Patients","volume":"11","author":"K Thieme","year":"2022","journal-title":"J Clin Med"},{"key":"pone.0317300.ref037","article-title":"Rethinking the Origin of the Primary Respiratory Mechanism","author":"B Bordoni","year":"2023","journal-title":"Cureus"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0317300","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T13:23:21Z","timestamp":1740662601000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0317300"}},"subtitle":[],"editor":[{"given":"Sanaullah","family":"Sajid","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,2,27]]},"references-count":37,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,2,27]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0317300","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,27]]}}}