{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T23:26:02Z","timestamp":1780097162516,"version":"3.54.0"},"reference-count":23,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T00:00:00Z","timestamp":1737936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>The oocyte retrieval is a critical step in assisted reproductive technologies, including in vitro fertilization and fertility preservation. Despite evolving techniques, the optimal aspiration pressure during retrieval remains debatable, with limited in vivo human studies. Existing studies, primarily in vitro and on animals, suggest that inappropriate aspiration pressures can impair oocyte quality. This study aims to compares the effects of two different aspiration pressures, 120mmHg and 150mmHg, on oocyte recovery, damage, and subsequent embryo development and pregnancy outcomes in infertile women undergoing transvaginal ultrasound-guided oocyte retrieval.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods and findings<\/jats:title>\n<jats:p>This retrospective study analyzed data from 891 women at Seoul National University Hospital between May 2018 and August 2023. A total of 400 cycles were included, with 202 at 120 mmHg and 198 at 150 mmHg aspiration pressures. The primary outcomes were the number of retrieved, matured, fractured oocytes, embryos, and good-grade embryos. Pregnancy outcomes were evaluated by comparing the clinical pregnancy rates and live birth rates. Univariate and multivariate logistic regression analyses were conducted to evaluate the relationship between aspiration pressure, clinical pregnancy, and live birth rates. There was statistically significant difference in the number of retrieved oocytes and mature oocytes between the 120 mmHg group and the 150 mmHg group (6.3\u00b15.2 vs. 7.7\u00b16.7, p = 0.018; 4.4\u00b13.7 vs. 5.6\u00b15.3, p = 0.011). The number of embryos and good grade embryos also differed significantly (3.3\u00b13.1 vs. 4.2\u00b13.9, p = 0.011; 1.0\u00b11.6 vs. 1.5\u00b12.6, p = 0.031). However, there were no significant differences in clinical pregnancy and live birth rates between the two groups in multivariate logistic regression analysis (adjusted OR = 0.725, p = 0.519; adjusted OR = 0.370, p = 0.170).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>Increasing the aspiration pressure to 150mmHg led to a higher yield of oocytes and embryos than 120mmHg, without any negative impact on oocyte quality or live birth rates. These findings provide valuable insights for clinical decision-making in infertility treatments, suggesting that 150mmHg may be a more effective pressure for oocyte retrieval in in vitro fertilization and embryo transfer.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0317812","type":"journal-article","created":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T18:36:56Z","timestamp":1738003016000},"page":"e0317812","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Optimal aspiration pressure of suction pump for oocyte retrieval in infertile patients undergoing in vitro fertilization"],"prefix":"10.1371","volume":"20","author":[{"given":"Sung Woo","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye Yun","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Yeon","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6423-854X","authenticated-orcid":true,"given":"Seung-Yup","family":"Ku","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,1,27]]},"reference":[{"issue":"4","key":"pone.0317812.ref001","doi-asserted-by":"crossref","first-page":"hoz025","DOI":"10.1093\/hropen\/hoz025","article-title":"Recommendations for good practice in ultrasound: oocyte pick up(\u2020).","volume":"2019","author":"A D\u2019Angelo","year":"2019","journal-title":"Hum Reprod Open"},{"issue":"10","key":"pone.0317812.ref002","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1016\/S0015-0282(16)39257-3","article-title":"Oocyte recovery from the human ovary","volume":"23","author":"LL Morgenstern","year":"1972","journal-title":"Fertil Steril"},{"issue":"6","key":"pone.0317812.ref003","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/S0015-0282(16)41171-4","article-title":"Laparoscopic recovery of mature human oocytes","volume":"26","author":"MJ Berger","year":"1975","journal-title":"Fertil Steril"},{"issue":"4","key":"pone.0317812.ref004","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/S0015-0282(16)45434-8","article-title":"The collection of human oocytes for in vitro fertilization. I. An instrument for maximizing oocyte recovery rate","volume":"35","author":"P Renou","year":"1981","journal-title":"Fertil Steril"},{"key":"pone.0317812.ref005","article-title":"Interventional Ultrasound: Standardisation of Oocyte Retrieval in Assisted Reproduction Treatments.","author":"C. 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Antosik JMJ","year":"2007","journal-title":"Archives Animal Breeding"},{"issue":"1","key":"pone.0317812.ref011","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1111\/j.1439-0531.2007.00866.x","article-title":"Effect of aspiration pressure during oocyte harvesting on oocyte recovery and in vitro development of ovine oocytes","volume":"43","author":"KM Morton","year":"2008","journal-title":"Reprod Domest Anim"},{"issue":"5","key":"pone.0317812.ref012","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1016\/j.rbmo.2019.06.013","article-title":"Is there an association between oocyte number and embryo quality? A systematic review and meta-analysis.","volume":"39","author":"BG Vermey","year":"2019","journal-title":"Reprod Biomed Online"},{"issue":"3","key":"pone.0317812.ref013","first-page":"556","article-title":"Cumulative live birth rates after one ART cycle including all subsequent frozen-thaw cycles in 1050 women: secondary outcome of an RCT comparing GnRH-antagonist and GnRH-agonist protocols","volume":"32","author":"M Toftager","year":"2017","journal-title":"Hum Reprod"},{"issue":"2","key":"pone.0317812.ref014","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.fertnstert.2016.10.037","article-title":"How many oocytes are optimal to achieve multiple live births with one stimulation cycle? The one-and-done approach","volume":"107","author":"DA Vaughan","year":"2017","journal-title":"Fertil Steril"},{"key":"pone.0317812.ref015","volume-title":"Textbook of Assisted Reproductive Techniques.","author":"DK Gardner","year":"2018","edition":"5"},{"key":"pone.0317812.ref016","volume-title":"An atlas of human gametes and conceptuses: an illustrated reference for assisted reproductive technology","author":"LL Veeck","year":"1999"},{"key":"pone.0317812.ref017","doi-asserted-by":"crossref","DOI":"10.3109\/9780203008935","volume-title":"An Atlas of Human Blastocysts","author":"LL Veeck","year":"2003"},{"issue":"2","key":"pone.0317812.ref018","doi-asserted-by":"crossref","first-page":"98","DOI":"10.4103\/0974-1208.158617","article-title":"Oocyte retrieval at 140-mmHg negative aspiration pressure: A promising alternative to flushing and aspiration in assisted reproduction in women with low ovarian reserve","volume":"8","author":"A Kumaran","year":"2015","journal-title":"J Hum Reprod Sci"},{"issue":"5","key":"pone.0317812.ref019","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1016\/0093-691X(96)00028-3","article-title":"Effects of aspiration vacuum and needle diameter on cumulus oocyte complex morphology and developmental capacity of bovine oocytes","volume":"45","author":"PE Bols","year":"1996","journal-title":"Theriogenology"},{"issue":"4","key":"pone.0317812.ref020","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/j.fertnstert.2018.04.039","article-title":"Cumulative live birth rates according to the number of oocytes retrieved after the first ovarian stimulation for in vitro fertilization\/intracytoplasmic sperm injection: a multicenter multinational analysis including \u223c15,000 women","volume":"110","author":"NP Polyzos","year":"2018","journal-title":"Fertil Steril"},{"key":"pone.0317812.ref021","doi-asserted-by":"crossref","first-page":"102307","DOI":"10.1016\/j.bpobgyn.2022.102307","article-title":"Association between the number of oocytes and cumulative live birth rate: A systematic review","volume":"87","author":"AR Neves","year":"2023","journal-title":"Best Pract Res Clin Obstet Gynaecol"},{"issue":"11","key":"pone.0317812.ref022","first-page":"2010","article-title":"Cumulative live birth rates and number of oocytes retrieved in women of advanced age. A single centre analysis including 4500 women \u226538 years old","volume":"33","author":"M Devesa","year":"2018","journal-title":"Hum Reprod"},{"issue":"4","key":"pone.0317812.ref023","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/S1472-6483(10)60891-7","article-title":"Effect of aspiration vacuum on the developmental competence of immature human oocytes retrieved using a 20-gauge needle","volume":"14","author":"S Hashimoto","year":"2007","journal-title":"Reprod Biomed Online"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0317812","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T18:37:07Z","timestamp":1738003027000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0317812"}},"subtitle":[],"editor":[{"given":"Gerrit J.","family":"Bouma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,1,27]]},"references-count":23,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,1,27]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0317812","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,27]]}}}