{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:02:49Z","timestamp":1787022169156,"version":"build-2736575974"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T00:00:00Z","timestamp":1744761600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Human migration significantly contributes to the global spread of infectious diseases. In recent years, Ethiopia has experienced an increased influx of returnees from the Kingdom of Saudi Arabia. These migrants were often held in densely populated and inadequately ventilated detention centers and faced a heightened risk of tuberculosis transmission. This study investigates the genotypic drug resistance and transmission clusters of\n                      <jats:italic>M. tuberculosis<\/jats:italic>\n                      among returnees from various detention centers in the Kingdom of Saudi Arabia. Whole genome sequencing (WGS) is employed to characterize the genotypic drug resistance patterns and transmission clusters of\n                      <jats:italic>M. tuberculosis<\/jats:italic>\n                      isolates in presumptive TB cases.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>\n                      Following symptom screening for presumptive TB, sputum samples were collected from 161 Xpert MTB\/RIF-confirmed returnees between August and December 2022. The samples were further cultured on Lowenstein-Jensen media, with 66.5% (107\/161) yielding positive results. The\n                      <jats:italic>M. tuberculosis<\/jats:italic>\n                      strains were classified, and genotypic drug susceptibility was predicted. Transmission clusters were identified using a distance threshold of 12 single nucleotide polymorphisms (SNPs).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Among the 88 isolates, Lineage 4 was the most prevalent, representing 65.9% of\n                      <jats:italic>M. tuberculosis<\/jats:italic>\n                      cases among returnees. Sub-lineage 4.2.2.2 was the most dominant within this group, comprising 33% (29\/88) of the isolates. Among the isolates, 40 gene mutations conferring resistance to first- and second-line anti-TB drugs were identified. Sixteen transmission clusters were identified, suggesting possible transmission events and a likely origin within the detention centers of the Kingdom of Saudi Arabia (KSA). The proportion of MDR-TB among newly diagnosed cases was 2.3% (2\/88).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The clustering patterns of\n                      <jats:italic>M. tuberculosis<\/jats:italic>\n                      strain among KSA returnees possibly suggest increased transmission rates in congregate settings. Most importantly, the identified prevalence of MDR-TB, particularly among newly diagnosed TB cases, underscores the need to strengthen robust screening practices for returning migrants before they reintegrate into the community to curb TB\/DR-TB transmission.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0318743","type":"journal-article","created":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T13:37:12Z","timestamp":1744810632000},"page":"e0318743","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Genotypic drug resistance and transmission clusters of Mycobacterium tuberculosis isolates among Ethiopian returnees from Saudi Arabia"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3101-3926","authenticated-orcid":true,"given":"Melaku","family":"Tilahun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abay","family":"Atnafu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tewodros 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