{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:33:44Z","timestamp":1792794824014,"version":"4.5.0"},"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T00:00:00Z","timestamp":1739232000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Research Council of Thailand through the NRCT Senior Research Scholar Program","award":["814-2020"],"award-info":[{"award-number":["814-2020"]}]},{"name":"King Mongkut\u2019s University of Technology North Bangkok and National Science and Technology Development Agency, Thailand","award":["020\/2563"],"award-info":[{"award-number":["020\/2563"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The identification of multifunctional peptides derived from marine byproducts represents a significant challenge in the field. In Thailand, the fisheries industry exports salted jellyfish, which results in low-value byproducts primarily employed for animal feed. Previous studies have indicated the bioactivities of jellyfish protein hydrolysates from <jats:italic>Lobonema smitthii<\/jats:italic>; however, the multifunctional properties of <jats:italic>Rhopilema hispidum<\/jats:italic> remain largely unexplored. This research aimed to characterize synthetic bioactive peptides sourced from the byproducts of salted jellyfish (<jats:italic>R. hispidum<\/jats:italic>), with a specific emphasis on their antioxidant, angiotensin-I-converting enzyme (ACE) inhibitory, and anti-inflammatory activities. The hydrolysate obtained from the umbrella portion, subjected to pepsin treatment at a 3:20 enzyme-to-substrate ratio for 48\u2009h at 37 \u00b0C, demonstrated the highest levels of antioxidant activity (DPPH =\u2009 1.85\u2009\u00b1\u20090.05\u2009mM TE\/mg protein, ABTS =\u2009 7.28\u2009\u00b1\u20090.03\u2009mM TE\/mg protein, FRAP =\u2009 3.04\u2009\u00b1\u20090.12\u2009mM FeSO<jats:sub>4<\/jats:sub>\/mg protein). Following purification, 18 novel peptides exhibiting high antioxidant scores (FRS+CHEL &gt;\u2009 0.48) were identified and synthesized. Notably, the peptide MVVACVLPEA exhibited significant antioxidant (DPPH =\u2009 56.07\u2009mM TE\/mg protein), ACE inhibitory (91.69%), and anti-inflammatory activities (NO release =\u2009 34.59 \u00b5\u2009M) without cytotoxic effects, although it is important to note that two other peptides did demonstrate cytotoxicity. This investigation reports a total of 16 synthesized peptides that possess triple functional activities\u2014antioxidant, ACE inhibitory, and anti-inflammatory\u2014without cytotoxicity, thus highlighting their potential applications in health-related fields.<\/jats:p>","DOI":"10.1371\/journal.pone.0318781","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T18:41:10Z","timestamp":1739299270000},"page":"e0318781","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Bioactivity assessment of peptides derived from salted jellyfish (Rhopilema hispidum) byproducts"],"prefix":"10.1371","volume":"20","author":[{"given":"Pratchaya","family":"Muangrod","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wiriya","family":"Charoenchokpanich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3696-8390","authenticated-orcid":true,"given":"Sittiruk","family":"Roytrakul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vilai","family":"Rungsardthong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sawanya","family":"Charoenlappanit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamaporn","family":"Wonganu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lueacha","family":"Tabtimmai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phumin","family":"Chamsodsai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Casanova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8511-5215","authenticated-orcid":true,"given":"Benjawan","family":"Thumthanaruk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,2,11]]},"reference":[{"key":"pone.0318781.ref001","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.jff.2015.09.031","article-title":"Purification and identification of antioxidant and ACE-inhibitory peptide from Saccharomyces cerevisiae protein hydrolysate","volume":"19","author":"M Mirzaei","year":"2015","journal-title":"J Funct Foods"},{"issue":"7","key":"pone.0318781.ref002","doi-asserted-by":"crossref","first-page":"e0200021","DOI":"10.1371\/journal.pone.0200021","article-title":"Purification and identification of two novel antioxidant peptides from perilla (Perilla frutescens L. 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