{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:02:48Z","timestamp":1787022168583,"version":"build-2736575974"},"reference-count":35,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T00:00:00Z","timestamp":1747958400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100021914","name":"Grand Challenges in Global Health","doi-asserted-by":"publisher","award":["INV-036234"],"award-info":[{"award-number":["INV-036234"]}],"id":[{"id":"10.13039\/100021914","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000278","name":"Department for International Development, UK Government","doi-asserted-by":"publisher","award":["MR\/L00464X\/1"],"award-info":[{"award-number":["MR\/L00464X\/1"]}],"id":[{"id":"10.13039\/501100000278","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Contaminated household water in peri-urban communities is a reservoir for virulent\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    , but its role in the environmental transmission of antibiotic resistance genes (ARGs) remains poorly understood. This study characterized\n                    <jats:italic>E. coli<\/jats:italic>\n                    from household water and additionally aimed to investigate the transmissibility of ARGs from drug-resistant isolates. Twenty-five\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates from thirteen household well water sources were tested for resistance to 14 antibiotics by disc diffusion and whole-genome sequenced using the Illumina platform. The ARGs and plasmid replicon types were respectively predicted using ResFinder and PlasmidFinder. Multidrug-resistant strains carrying plasmid replicons found in unrelated strains were conjugated with nalidixic acid-resistant (NAL\n                    <jats:sup>R<\/jats:sup>\n                    )\n                    <jats:italic>E. coli<\/jats:italic>\n                    C\n                    <jats:sub>600<\/jats:sub>\n                    using the solid plate method. Fifteen isolates displayed a multi-drug resistance (MDR) phenotype, with 18 possessing ARGs that confer resistance to trimethoprim-sulfamethoxazole, macrolide, sulphonamide, aminoglycoside, chloramphenicol, \u03b2-lactams, and tetracycline. Fifteen of the 25 isolates belonged to sequence type detected more than once, and fourteen of these were multidrug resistant. Through solid plate mating, beta-lactam-resistant\n                    <jats:italic>qnrS1-tet-dfrA14-<\/jats:italic>\n                    positive strains bearing IncFI-, IncHI2, and IncHI2A successfully transferred ampicillin resistance to a nalidixic acid-resistant derivative of\n                    <jats:italic>E. coli<\/jats:italic>\n                    -\n                    <jats:sub>C600<\/jats:sub>\n                    . This research highlights the urgent need to safeguard household water sources against fecal contamination to curb the dissemination of ARGs among bacterial populations in this environment.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0318969","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T13:43:47Z","timestamp":1748007827000},"page":"e0318969","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["Transmissible antimicrobial resistance in Escherichia coli isolated from household drinking water in Ibadan, Nigeria"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6232-7680","authenticated-orcid":true,"given":"Akeem G.","family":"Rabiu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6268-2532","authenticated-orcid":true,"given":"Olutayo 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Sumrall","year":"2014","journal-title":"PLoS One"},{"key":"pone.0318969.ref013","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1016\/j.jgar.2020.04.010","article-title":"A systematic review on the prevalence of extended-spectrum beta lactamase-producing Gram-negative bacteria in Nigeria","volume":"22","author":"N Tanko","year":"2020","journal-title":"J Glob Antimicrob Resist"},{"issue":"2","key":"pone.0318969.ref014","doi-asserted-by":"crossref","DOI":"10.24940\/theijhss\/2020\/v8\/i2\/HS2002-049","article-title":"Urban well characteristics and groundwater pollution in Ibadan, Nigeria","volume":"8","author":"SA Adejumo","year":"2020","journal-title":"theijhss"},{"key":"pone.0318969.ref015","author":"Raosoft.","year":"2004"},{"issue":"4","key":"pone.0318969.ref016","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1093\/genetics\/39.4.440","article-title":"Segregation of new lysogenic types during growth of a doubly lysogenic strain derived from Escherichia Coli 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Page","year":"2016","journal-title":"Microb Genom"},{"issue":"7","key":"pone.0318969.ref021","doi-asserted-by":"crossref","first-page":"3895","DOI":"10.1128\/AAC.02412-14","article-title":"In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing","volume":"58","author":"A Carattoli","year":"2014","journal-title":"Antimicrob Agents Chemother"},{"issue":"11","key":"pone.0318969.ref022","doi-asserted-by":"crossref","first-page":"2640","DOI":"10.1093\/jac\/dks261","article-title":"Identification of acquired antimicrobial resistance genes","volume":"67","author":"E Zankari","year":"2012","journal-title":"J Antimicrob Chemother"},{"issue":"10","key":"pone.0318969.ref023","article-title":"ARIBA: rapid antimicrobial resistance genotyping directly from sequencing reads","volume":"3","author":"M Hunt","year":"2017","journal-title":"Microb 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Dis"},{"issue":"12","key":"pone.0318969.ref027","article-title":"An ST131 clade and a phylogroup A clade bearing an O101-like O-antigen cluster predominate among bloodstream Escherichia coli isolates from South-West Nigeria hospitals","volume":"8","author":"AO Afolayan","year":"2022","journal-title":"Microb Genom"},{"issue":"5","key":"pone.0318969.ref028","doi-asserted-by":"crossref","DOI":"10.1093\/jacamr\/dlac093","article-title":"Antibiotic utilization study in a teaching hospital in Nigeria","volume":"4","author":"KF Sekoni","year":"2022","journal-title":"JAC Antimicrob Resist"},{"key":"pone.0318969.ref029","doi-asserted-by":"crossref","first-page":"811","DOI":"10.2147\/IDR.S401663","article-title":"Quinolone antibiotics: resistance and therapy","volume":"16","author":"K Tang","year":"2023","journal-title":"Infect Drug Resist"},{"key":"pone.0318969.ref030","doi-asserted-by":"crossref","first-page":"547","DOI":"10.3389\/fmicb.2020.00547","article-title":"Corrigendum: transduction as 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