{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:02:49Z","timestamp":1787022169610,"version":"build-2736575974"},"reference-count":18,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T00:00:00Z","timestamp":1740441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hangzhou Biomedicine and Health Industry Development Supporting Science and Technology Special Project","award":["2023WJC162"],"award-info":[{"award-number":["2023WJC162"]}]},{"name":"Hangzhou Biomedicine and Health Industry Development Supporting Science and Technology Special Project","award":["2023WJC312"],"award-info":[{"award-number":["2023WJC312"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Early, rapid and precise diagnosis of tuberculous pleurisy is difficult. Next-generation sequencing (NGS) has been used for the diagnosis of TB in some high-income countries. We completed the protocol of the study in order to better guide the systematic review and meta-analysis for evaluating the role of NGS in the diagnosis of tuberculous pleurisy, which was the purpose of the current study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We designed and drafted this protocol as per the Preferred reporting items for systematic review and meta-analysis protocols (PRISMA-P) guidelines. Three English databases (Cochrane Library, Embase and Medline via PubMed) and two Chinese databases (China National Knowledge Infrastructure and Wanfang Database) will be used to search for relevant studies. We followed the PICT principles to develop the eligibility criteria. Once the final compliant literature is identified, we will extract the valid information from the literature for statistical analysis. We will assess the quality of the included studies using the Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) tool. When there are more than 4 eligible studies, we will use Stata (version 15.0; Stata Corp., College Station, TX, the USA) with\n                      <jats:italic>midas<\/jats:italic>\n                      module to conduct the relevant meta-analysis and plot the relevant forest plots and curves. When there are less than 4 eligible studies, we will use Meta-DiSc (version 1.4) to perform the associated meta-analysis and plot the associated forest plots and curves. We will use the\n                      <jats:italic>I<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      statistic to assess heterogeneity between included studies. We will conduct meta-regression analysis, subgroup analysis and sensitivity analysis to explore possible sources of heterogeneity when significant heterogeneity exists.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Systematic review registration<\/jats:title>\n                    <jats:p>PROSPERO Registration number: CRD42024568791<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0319175","type":"journal-article","created":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T13:27:22Z","timestamp":1740490042000},"page":"e0319175","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Accuracy of next-generation sequencing for rapid diagnosis of tuberculous pleurisy: A protocol of systematic review and meta-analysis"],"prefix":"10.1371","volume":"20","author":[{"given":"Yuyang","family":"Ling","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanqin","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xudong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8570-8013","authenticated-orcid":true,"given":"Guocan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,2,25]]},"reference":[{"issue":"11","key":"pone.0319175.ref001","doi-asserted-by":"crossref","first-page":"e0289336","DOI":"10.1371\/journal.pone.0289336","article-title":"Meta-analysis of diagnostic accuracy of nucleic acid amplification tests for abdominal tuberculosis","volume":"18","author":"Y Shen","year":"2023","journal-title":"PLoS One"},{"key":"pone.0319175.ref002","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.2147\/IDR.S405868","article-title":"Clinical application of the mycobacterium tuberculosis-RNA assay of pericardial tissue specimens in the diagnosis of tuberculous pericarditis","volume":"16","author":"J Zhang","year":"2023","journal-title":"Infect Drug Resist"},{"issue":"3","key":"pone.0319175.ref003","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1097\/MCP.0000000000001052","article-title":"Tuberculous pleuritis: clinical presentations and diagnostic challenges","volume":"30","author":"KKP Chan","year":"2024","journal-title":"Curr Opin Pulm Med"},{"issue":"4","key":"pone.0319175.ref004","doi-asserted-by":"crossref","first-page":"230143","DOI":"10.1183\/20734735.0143-2023","article-title":"The tuberculous pleural effusion","volume":"19","author":"E McNally","year":"2023","journal-title":"Breathe (Sheffield, England)"},{"key":"pone.0319175.ref005","doi-asserted-by":"crossref","first-page":"100354","DOI":"10.1016\/j.jctube.2023.100354","article-title":"Serosal membrane tuberculosis in Iran: A comprehensive review of evidences","volume":"31","author":"A Ebrahimzadeh","year":"2023","journal-title":"J Clin Tubercul Other Mycobact Dis"},{"issue":"4","key":"pone.0319175.ref006","first-page":"e59300","article-title":"Validity of Pleuroscopy in Evaluating Pleural Effusions of Uncertain Etiology at the Cardiothoracic Unit of Minia University Hospital, Egypt","volume":"16","author":"A Abdelaziz","year":"2024","journal-title":"Cureus"},{"issue":"1","key":"pone.0319175.ref007","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1186\/s12967-023-04492-x","article-title":"Simultaneous diagnosis of tuberculous pleurisy and malignant pleural effusion using metagenomic next-generation sequencing (mNGS)","volume":"21","author":"F Xu","year":"2023","journal-title":"J Transl Med"},{"issue":"9","key":"pone.0319175.ref008","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.1016\/j.cmi.2024.05.012","article-title":"Evaluation of droplet digital polymerase chain reaction by detecting cell-free deoxyribonucleic acid in pleural effusion for the diagnosis of tuberculous pleurisy: a multicentre cohort study","volume":"30","author":"F Xu","year":"2024","journal-title":"Clin Microbiol Infect"},{"issue":"20","key":"pone.0319175.ref009","doi-asserted-by":"crossref","first-page":"e29363","DOI":"10.1097\/MD.0000000000029363","article-title":"Head-to-head comparison of the efficacy of Xpert MTB\/RIF Ultra and Xpert MTB\/RIF for the diagnosis of tuberculous pleurisy: A systematic review and meta-analysis","volume":"101","author":"W Yu","year":"2022","journal-title":"Medicine (Baltimore)"},{"key":"pone.0319175.ref010","article-title":"An overview of next generation sequencing strategies and genomics tools used for tuberculosis research","author":"S Deb","year":"2024","journal-title":"J Appl Microbiol"},{"key":"pone.0319175.ref011","doi-asserted-by":"crossref","first-page":"1224993","DOI":"10.3389\/fpubh.2023.1224993","article-title":"Metagenomic next-generation sequencing for Mycobacterium tuberculosis complex detection: a meta-analysis","volume":"11","author":"Y Li","year":"2023","journal-title":"Front Public Health"},{"key":"pone.0319175.ref012","first-page":"g7647","article-title":"Preferred reporting items for systematic review and meta-analysis protocols (PRISMA-P) 2015: elaboration and explanation","volume":"350","author":"PRISMA-P Group","year":"2015","journal-title":"BMJ (Clinical Research ed)"},{"issue":"4","key":"pone.0319175.ref013","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1001\/jama.2017.19163","article-title":"Preferred reporting items for a systematic review and meta-analysis of diagnostic test accuracy studies: the PRISMA-DTA statement","volume":"319","author":"and the PRISMA-DTA 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meta-analysis","volume":"16","author":"G Yu","year":"2021","journal-title":"PLoS One"},{"issue":"4","key":"pone.0319175.ref017","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.jclinepi.2010.09.011","article-title":"GRADE guidelines: a new series of articles in the Journal of Clinical Epidemiology","volume":"64","author":"GH Guyatt","year":"2011","journal-title":"J Clin Epidemiol"},{"issue":"6","key":"pone.0319175.ref018","doi-asserted-by":"crossref","first-page":"e0304162","DOI":"10.1371\/journal.pone.0304162","article-title":"Diagnostic accuracy of nanopore sequencing for the rapid diagnosis of pulmonary tuberculosis: A protocol for a systematic review and meta-analysis","volume":"19","author":"G Yu","year":"2024","journal-title":"PLoS One"}],"container-title":["PLOS 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