{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T18:51:38Z","timestamp":1783191098372,"version":"3.54.6"},"reference-count":91,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T00:00:00Z","timestamp":1743120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Ebola virus (EBOV) is a filovirus that causes severe hemorrhagic fever and has a fatality rate between 50 and 90%. The vaccines were developed against the Ebola Zaire species; therefore, it is necessary to develop vaccines against other species to control future outbreaks. The objective of this work was to obtain vaccine candidate peptides against different EBOV species through the use of bioinformatics programs and servers that allow glycoprotein (GP) to be analyzed. GP sequences of various EBOV species that did not present gaps or unspecified amino acids or that were repeated (same year, region and laboratory) were downloaded from the NCBI database. A consensus sequence was generated and used to determine vaccine candidate peptides, which were evaluated, through a combination of servers and molecular dynamics, for their ability to interact with B and T lymphocytes, toxicity, allergenicity, solvent exposure, glycosylation, antigenicity, and presence in mature GP. Five vaccine candidate peptides were identified, of which PEP4 had the best characteristics evaluated in this study. PEP4 may be a potential candidate for the development of an EBOV vaccine.<\/jats:p>","DOI":"10.1371\/journal.pone.0319496","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T18:15:29Z","timestamp":1743185729000},"page":"e0319496","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["In silico design of Ebola virus Glycoprotein antigenic peptides as vaccine candidates"],"prefix":"10.1371","volume":"20","author":[{"given":"David","family":"Lara-Ram\u00edrez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara Esperanza","family":"Santacruz-Tinoco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Ram\u00f3n-Gallegos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1289-4457","authenticated-orcid":true,"given":"Jos\u00e9 Esteban","family":"Mu\u00f1oz-Medina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,3,28]]},"reference":[{"issue":"5","key":"pone.0319496.ref001","first-page":"1229","article-title":"Virus nomenclature below the species level: a standardized nomenclature for filovirus strains and variants rescued from cDNA","volume":"159","author":"JH Kuhn","year":"2014","journal-title":"Arch Virol"},{"issue":"19","key":"pone.0319496.ref002","doi-asserted-by":"crossref","first-page":"3553","DOI":"10.1093\/bioinformatics\/btz267","article-title":"Is the Bombali virus pathogenic in humans?","volume":"35","author":"HJ Martell","year":"2019","journal-title":"Bioinformatics"},{"key":"pone.0319496.ref003","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1146\/annurev-pathol-052016-100506","article-title":"The Pathogenesis of Ebola Virus Disease","volume":"12","author":"L Baseler","year":"2017","journal-title":"Annu Rev Pathol"},{"key":"pone.0319496.ref004","first-page":"666","article-title":"Familia Filoviridae","volume-title":"Microbiolog\u00eda. Bacteriolog\u00eda y Virolog\u00eda, 2nd ed","author":"M Manjarrez-Zavala","year":"2010"},{"issue":"2","key":"pone.0319496.ref005","first-page":"271","article-title":"Ebola haemorrhagic fever in Zaire, 1976","volume":"56","author":"Report of an International Commission","year":"1978","journal-title":"Bull World Health Organ"},{"issue":"1721","key":"pone.0319496.ref006","doi-asserted-by":"crossref","first-page":"20160297","DOI":"10.1098\/rstb.2016.0297","article-title":"The Ebola outbreak, 2013-2016: old lessons for new epidemics","volume":"372","author":"CEM Coltart","year":"2017","journal-title":"Philos Trans R Soc Lond B Biol Sci"},{"issue":"45","key":"pone.0319496.ref007","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.15585\/mmwr.mm7145a5","article-title":"Notes from the Field: Outbreak of Ebola Virus Disease Caused by Sudan ebolavirus - Uganda, August-October 2022","volume":"71","author":"T Kiggundu","year":"2022","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"pone.0319496.ref008","article-title":"Vaccine against Ebola: Commission grants first ever market authorisation. 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Sci"},{"issue":"4","key":"pone.0319496.ref026","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1093\/bioinformatics\/btv639","article-title":"Gapped sequence alignment using artificial neural networks: application to the MHC class I system","volume":"32","author":"M Andreatta","year":"2016","journal-title":"Bioinformatics"},{"issue":"Suppl 1","key":"pone.0319496.ref027","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s12864-017-4328-8","article-title":"Mapping HLA-A2, -A3 and -B7 supertype-restricted T-cell epitopes in the ebolavirus proteome","volume":"19","author":"WC Lim","year":"2018","journal-title":"BMC Genomics"},{"issue":"6","key":"pone.0319496.ref028","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1007\/s00251-010-0441-4","article-title":"NetCTLpan: pan-specific MHC class I pathway epitope predictions","volume":"62","author":"T Stranzl","year":"2010","journal-title":"Immunogenetics"},{"issue":"23\u201324","key":"pone.0319496.ref029","doi-asserted-by":"crossref","first-page":"3195","DOI":"10.1016\/j.vaccine.2004.02.005","article-title":"Prediction of CTL epitopes using QM, SVM and ANN techniques","volume":"22","author":"M Bhasin","year":"2004","journal-title":"Vaccine"},{"issue":"1","key":"pone.0319496.ref030","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vacun.2020.09.005","article-title":"Glycosylation, ligand binding sites and antigenic variations between membrane glycoprotein of COVID-19 and related coronaviruses","volume":"22","author":"AA Dawood","year":"2021","journal-title":"Vacunas"},{"issue":"6","key":"pone.0319496.ref031","doi-asserted-by":"crossref","first-page":"3210","DOI":"10.3390\/ijms22063210","article-title":"Linear B-Cell Epitope Prediction for In Silico Vaccine Design: A Performance Review of Methods Available via Command-Line Interface","volume":"22","author":"KA Galanis","year":"2021","journal-title":"Int J Mol Sci"},{"key":"pone.0319496.ref032","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/978-3-540-30220-9_16","article-title":"BcePred: Prediction of Continuous B-Cell Epitopes in Antigenic Sequences Using Physico-chemical Properties","author":"S Saha","year":"2004","journal-title":"Artificial Immune Systems"},{"issue":"W1","key":"pone.0319496.ref033","doi-asserted-by":"crossref","first-page":"W24","DOI":"10.1093\/nar\/gkx346","article-title":"BepiPred-2.0: improving sequence-based B-cell epitope prediction using conformational epitopes","volume":"45","author":"MC Jespersen","year":"2017","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"pone.0319496.ref034","first-page":"193","article-title":"B-Cell conserved epitope screening and in silico cloning of envelope glycoprotein from Ebola virus (EBOV) for vaccine candidate construction","volume":"34","author":"T Widjaja","year":"2023","journal-title":"Indonesian Journal of 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Dimitrov","year":"2014","journal-title":"Bioinformatics"},{"issue":"7","key":"pone.0319496.ref038","doi-asserted-by":"crossref","first-page":"2064","DOI":"10.1093\/bioinformatics\/btac019","article-title":"SSpro\/ACCpro 6: almost perfect prediction of protein secondary structure and relative solvent accessibility using profiles, deep learning and structural similarity","volume":"38","author":"G Urban","year":"2022","journal-title":"Bioinformatics"},{"issue":"7610","key":"pone.0319496.ref039","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1038\/nature18615","article-title":"Toremifene interacts with and destabilizes the Ebola virus glycoprotein","volume":"535","author":"Y Zhao","year":"2016","journal-title":"Nature"},{"key":"pone.0319496.ref040","first-page":"310","article-title":"Prediction of glycosylation across the human proteome and the correlation to protein function","author":"R Gupta","year":"2002","journal-title":"Pac Symp Biocomput"},{"issue":"W1","key":"pone.0319496.ref041","doi-asserted-by":"crossref","first-page":"W449-54","DOI":"10.1093\/nar\/gkw329","article-title":"PEP-FOLD3: faster de novo structure prediction for linear peptides in solution and in complex","volume":"44","author":"A Lamiable","year":"2016","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"pone.0319496.ref042","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/jcc.21334","article-title":"AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading","volume":"31","author":"O Trott","year":"2010","journal-title":"J Comput Chem"},{"key":"pone.0319496.ref043","article-title":"Ebola virus disease. World Health Organization Newsroom","author":"World Health Organization"},{"issue":"7877","key":"pone.0319496.ref044","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1038\/d41586-021-02378-w","article-title":"Ebola virus can lie low and reactivate after years in human survivors","volume":"597","author":"RF Garry","year":"2021","journal-title":"Nature"},{"issue":"3","key":"pone.0319496.ref045","doi-asserted-by":"crossref","first-page":"181","DOI":"10.5152\/tpa.2014.2206","article-title":"Vaccination in patients with immunosuppression","volume":"49","author":"A Arvas","year":"2014","journal-title":"Turk Pediatri Ars"},{"issue":"10","key":"pone.0319496.ref046","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.3390\/pathogens10101330","article-title":"Structural and Functional Aspects of Ebola Virus Proteins","volume":"10","author":"S Jain","year":"2021","journal-title":"Pathogens"},{"issue":"4","key":"pone.0319496.ref047","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/S1473-3099(20)30515-6","article-title":"Vaccine innovation spurred by the long wait for an Ebola virus vaccine","volume":"21","author":"H Fausther-Bovendo","year":"2021","journal-title":"Lancet Infect Dis"},{"key":"pone.0319496.ref048","doi-asserted-by":"crossref","first-page":"442","DOI":"10.3389\/fimmu.2020.00442","article-title":"Better Epitope Discovery, Precision Immune Engineering, and Accelerated Vaccine Design Using Immunoinformatics Tools","volume":"11","author":"AS De Groot","year":"2020","journal-title":"Front Immunol"},{"issue":"11","key":"pone.0319496.ref049","doi-asserted-by":"crossref","first-page":"1844","DOI":"10.3390\/vaccines10111844","article-title":"The Advantage of Using Immunoinformatic Tools on Vaccine Design and Development for Coronavirus","volume":"10","author":"J Garc\u00eda-Machorro","year":"2022","journal-title":"Vaccines (Basel)"},{"key":"pone.0319496.ref050","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.jtbi.2019.01.016","article-title":"Computational analysis in designing T cell epitopes enriched peptides of Ebola glycoprotein exhibiting strong binding interaction with HLA molecules","volume":"465","author":"S Jain","year":"2019","journal-title":"J Theor Biol"},{"issue":"8","key":"pone.0319496.ref051","doi-asserted-by":"crossref","first-page":"925","DOI":"10.3390\/vaccines9080925","article-title":"Immunoinformatics Approach to Design Multi-Epitope- Subunit Vaccine against Bovine Ephemeral Fever Disease","volume":"9","author":"S Pyasi","year":"2021","journal-title":"Vaccines (Basel)"},{"issue":"4","key":"pone.0319496.ref052","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1080\/14760584.2022.2021882","article-title":"Exploring Klebsiella pneumoniae capsule polysaccharide proteins to design multiepitope subunit vaccine to fight against pneumonia","volume":"21","author":"J Dey","year":"2022","journal-title":"Expert Rev Vaccines"},{"issue":"1","key":"pone.0319496.ref053","doi-asserted-by":"crossref","first-page":"10895","DOI":"10.1038\/s41598-020-67749-1","article-title":"A candidate multi-epitope vaccine against SARS-CoV-2","volume":"10","author":"T Kar","year":"2020","journal-title":"Sci Rep"},{"issue":"4","key":"pone.0319496.ref054","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1007\/s10989-023-10535-0","article-title":"Advances in Computational and Bioinformatics Tools and Databases for Designing and Developing a Multi-Epitope-Based Peptide Vaccine","volume":"29","author":"MMAK Shawan","year":"2023","journal-title":"Int J Pept Res Ther"},{"issue":"5","key":"pone.0319496.ref055","doi-asserted-by":"crossref","first-page":"2369","DOI":"10.1080\/07391102.2020.1838329","article-title":"Computationally validated SARS-CoV-2 CTL and HTL Multi-Patch vaccines, designed by reverse epitomics approach, show potential to cover large ethnically distributed human population worldwide","volume":"40","author":"S Srivastava","year":"2022","journal-title":"J Biomol Struct Dyn"},{"key":"pone.0319496.ref056","doi-asserted-by":"crossref","first-page":"105462","DOI":"10.1016\/j.compbiomed.2022.105462","article-title":"Subtractive proteomics assisted therapeutic targets mining and designing ensemble vaccine against Candida auris for immune response induction","volume":"145","author":"T Khan","year":"2022","journal-title":"Comput Biol Med"},{"issue":"1","key":"pone.0319496.ref057","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1038\/icb.2010.65","article-title":"Innovative bioinformatic approaches for developing peptide-based vaccines against hypervariable viruses","volume":"89","author":"D Sirskyj","year":"2011","journal-title":"Immunol Cell 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Microbiol"},{"issue":"3","key":"pone.0319496.ref061","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1038\/nri3802","article-title":"Memory B cells","volume":"15","author":"T Kurosaki","year":"2015","journal-title":"Nat Rev Immunol"},{"issue":"2","key":"pone.0319496.ref062","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.immuni.2018.01.007","article-title":"Human T Cell Development, Localization, and Function throughout Life","volume":"48","author":"BV Kumar","year":"2018","journal-title":"Immunity"},{"issue":"32","key":"pone.0319496.ref063","doi-asserted-by":"crossref","first-page":"E7578","DOI":"10.1073\/pnas.1806200115","article-title":"Analysis of CD8+ T cell response during the 2013-2016 Ebola epidemic in West Africa","volume":"115","author":"S Sakabe","year":"2018","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"12","key":"pone.0319496.ref064","doi-asserted-by":"crossref","first-page":"2953","DOI":"10.1080\/21645515.2017.1360454","article-title":"Combinatorial peptide-based epitope mapping from Ebola virus DNA vaccines and infections reveals residue-level determinants of antibody binding","volume":"13","author":"DR Ripoll","year":"2017","journal-title":"Hum Vaccin Immunother"},{"issue":"10","key":"pone.0319496.ref065","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1038\/s41591-019-0602-4","article-title":"Polyclonal and convergent antibody response to Ebola virus vaccine rVSV-ZEBOV","volume":"25","author":"SA Ehrhardt","year":"2019","journal-title":"Nat Med"},{"issue":"9","key":"pone.0319496.ref066","doi-asserted-by":"crossref","first-page":"2537-2545.e3","DOI":"10.1016\/j.celrep.2019.10.105","article-title":"Characterization of Antigenic MHC-Class-I-Restricted T Cell Epitopes in the Glycoprotein of Ebolavirus","volume":"29","author":"J Powlson","year":"2019","journal-title":"Cell Rep"},{"issue":"6","key":"pone.0319496.ref067","doi-asserted-by":"crossref","first-page":"e96360","DOI":"10.1371\/journal.pone.0096360","article-title":"Identification of continuous human B-cell epitopes in the VP35, VP40, nucleoprotein and glycoprotein of Ebola virus","volume":"9","author":"P Becquart","year":"2014","journal-title":"PLoS One"},{"issue":"4","key":"pone.0319496.ref068","doi-asserted-by":"crossref","first-page":"1050-1059.e5","DOI":"10.1016\/j.celrep.2018.06.077","article-title":"Qualitative profiling of the humoral immune response elicited by rVSV-\u0394G-EBOV-GP using a systems serology assay, domain programmable arrays","volume":"24","author":"M Sanchez-Lockhart","year":"2018","journal-title":"Cell Rep"},{"issue":"6","key":"pone.0319496.ref069","doi-asserted-by":"crossref","first-page":"1566-1582.e17","DOI":"10.1016\/j.cell.2019.04.036","article-title":"Longitudinal analysis of the human B cell response to ebola virus 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(Basel)"},{"key":"pone.0319496.ref073","doi-asserted-by":"crossref","first-page":"107688","DOI":"10.1016\/j.compbiolchem.2022.107688","article-title":"A new approach for determining SARS-CoV-2 epitopes using machine learning-based in silico methods","volume":"98","author":"P Cihan","year":"2022","journal-title":"Comput Biol Chem"},{"issue":"2","key":"pone.0319496.ref074","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1002\/wcms.1240","article-title":"In silico toxicology: computational methods for the prediction of chemical toxicity","volume":"6","author":"AB Raies","year":"2016","journal-title":"Wiley Interdiscip Rev Comput Mol Sci"},{"issue":"1","key":"pone.0319496.ref075","doi-asserted-by":"crossref","first-page":"2688","DOI":"10.1038\/s41467-020-16567-0","article-title":"Vulnerabilities in coronavirus glycan shields despite extensive glycosylation","volume":"11","author":"Y Watanabe","year":"2020","journal-title":"Nat Commun"},{"issue":"24","key":"pone.0319496.ref076","doi-asserted-by":"crossref","first-page":"12463","DOI":"10.1128\/JVI.76.24.12463-12472.2002","article-title":"Covalent modifications of the ebola virus glycoprotein","volume":"76","author":"SA Jeffers","year":"2002","journal-title":"J Virol"},{"issue":"7","key":"pone.0319496.ref077","doi-asserted-by":"crossref","first-page":"797","DOI":"10.3390\/vaccines9070797","article-title":"Bioinformatics Prediction of SARS-CoV-2 Epitopes as Vaccine Candidates for the Colombian Population","volume":"9","author":"D Montes-Grajales","year":"2021","journal-title":"Vaccines (Basel)"},{"issue":"5","key":"pone.0319496.ref078","doi-asserted-by":"crossref","first-page":"2302","DOI":"10.1021\/acs.jcim.1c00159","article-title":"Benchmark of Popular Free Energy Approaches Revealing the Inhibitors Binding to SARS-CoV-2 Mpro","volume":"61","author":"ST Ngo","year":"2021","journal-title":"J Chem Inf 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