{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T08:27:16Z","timestamp":1782289636270,"version":"3.54.5"},"reference-count":73,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2122799"],"award-info":[{"award-number":["2122799"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006502","name":"Defense Sciences Office, DARPA","doi-asserted-by":"publisher","award":["HR0011-18-9-0007"],"award-info":[{"award-number":["HR0011-18-9-0007"]}],"id":[{"id":"10.13039\/100006502","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Climate change is increasing the need to limit levels of anthropogenic CO\n                    <jats:sub>2<\/jats:sub>\n                    released into the atmosphere. One approach being investigated is to generate products based on microbially induced carbonate precipitation (MICP), which can trap CO\n                    <jats:sub>2<\/jats:sub>\n                    as CaCO\n                    <jats:sub>3<\/jats:sub>\n                    . We recently identified a novel MICP pathway in bacteria that is initiated by Ca\n                    <jats:sup>2+<\/jats:sup>\n                    toxicity in cells, causing extracellular CO\n                    <jats:sub>2<\/jats:sub>\n                    to be trapped as CO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>2-<\/jats:sup>\n                    by\n                    <jats:italic>Escherichia coli,<\/jats:italic>\n                    although the yield of precipitated CaCO\n                    <jats:sub>3<\/jats:sub>\n                    remained low (in the milligram range). In this work, we used the\n                    <jats:italic>E. coli<\/jats:italic>\n                    Keio gene knock-out library to identify 54 genes involved in MICP in\n                    <jats:italic>E. coli,<\/jats:italic>\n                    which could be broadly characterized into four groups: central metabolism, iron metabolism, cell architecture, and transport. The role of central metabolism appears to be crucial in maintaining alkaline conditions surrounding the cell that promote CaCO\n                    <jats:sub>3<\/jats:sub>\n                    precipitation. The role of iron metabolism was less clear, although the results suggest that growth rate influences the initiation of MICP. While the impact of repeating polymeric structures on cell surfaces promoting MICP is well established, our results suggest that other structural features may play a role, including fimbriae and flagella. Finally, the results confirmed that Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transport is central to MICP under calcium stress. The results further suggest that the ZntB efflux pump may play a previously unidentified role in Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transport in\n                    <jats:italic>E. coli.<\/jats:italic>\n                    By overexpressing some of these genes, our work suggests that there are several previously unidentified cellular mechanisms that could serve as a target for enhanced MICP in\n                    <jats:italic>E. coli.<\/jats:italic>\n                    By incorporating these processes into MICP pathways in\n                    <jats:italic>E. coli,<\/jats:italic>\n                    it may be possible to increase the volume of CO\n                    <jats:sub>2<\/jats:sub>\n                    fixed using this pathway and yield potentially new products that can replace CO\n                    <jats:sub>2<\/jats:sub>\n                    intensive products, such as precipitated calcium carbonates (PCCs) for industry.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0319530","type":"journal-article","created":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T18:26:21Z","timestamp":1765909581000},"page":"e0319530","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["The diversity of cellular systems involved in carbonate precipitation by Escherichia coli"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8832-6630","authenticated-orcid":true,"given":"Matthew E.","family":"Jennings","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George J.","family":"Breley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reilly S.","family":"Blackwell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Drabik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen R.","family":"Gisser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joe","family":"Kainrad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Ligman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaycie","family":"Proctor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8070-5767","authenticated-orcid":true,"given":"Rose","family":"Deshler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian A.","family":"O\u2019Hart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arlyn","family":"Rivera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorelei","family":"Centrella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Bonn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bisma","family":"Chaudhry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8585-9997","authenticated-orcid":true,"given":"Hazel A.","family":"Barton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,12,16]]},"reference":[{"issue":"2","key":"pone.0319530.ref001","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1093\/icb\/44.2.163","article-title":"Polar bears in a warming climate","volume":"44","author":"AE Derocher","year":"2004","journal-title":"Integr Comp Biol"},{"key":"pone.0319530.ref002","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1016\/j.crte.2008.07.002","article-title":"Research frontiers in climate change: effects of extreme meteorological events on ecosystems","volume":"340","author":"A Jentsch","year":"2008","journal-title":"Comptes Rendus G\u00e9oscience"},{"issue":"6","key":"pone.0319530.ref003","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1038\/nclimate1389","article-title":"Physically based assessment of hurricane surge threat under climate change","volume":"2","author":"N Lin","year":"2012","journal-title":"Nature Clim Change"},{"issue":"1","key":"pone.0319530.ref004","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.gloenvcha.2003.10.008","article-title":"Effects of climate change on global food production under SRES emissions and socio-economic scenarios","volume":"14","author":"ML Parry","year":"2004","journal-title":"Global Environ Change"},{"key":"pone.0319530.ref005","doi-asserted-by":"crossref","first-page":"137782","DOI":"10.1016\/j.scitotenv.2020.137782","article-title":"Climate change effects on biodiversity, ecosystems, ecosystem services, and natural resource management in the United States","volume":"733","author":"SR Weiskopf","year":"2020","journal-title":"Sci Total Environ"},{"key":"pone.0319530.ref006","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.geoderma.2006.09.003","article-title":"How strongly can forest management influence soil carbon sequestration?","volume":"137","author":"R Jandl","year":"2007","journal-title":"Geoderma"},{"issue":"1","key":"pone.0319530.ref007","doi-asserted-by":"crossref","first-page":"3641","DOI":"10.1016\/j.egypro.2009.02.160","article-title":"Permanent Carbon Dioxide Storage into Basalt: The CarbFix Pilot Project, Iceland","volume":"1","author":"JM Matter","year":"2009","journal-title":"Energy Procedia"},{"key":"pone.0319530.ref008","doi-asserted-by":"crossref","DOI":"10.3389\/fenrg.2017.00017","article-title":"Calcium carbonate precipitation for CO2 storage and utilization: a review of the carbonate crystallization and polymorphism","volume":"5","author":"R Chang","year":"2017","journal-title":"Front Energy Res"},{"key":"pone.0319530.ref009","doi-asserted-by":"crossref","first-page":"121372","DOI":"10.1016\/j.jclepro.2020.121372","article-title":"Microbiologically Induced Calcite Precipitation biocementation, green alternative for roads \u2013 is this the breakthrough? A critical review","volume":"262","author":"CRM Portugal","year":"2020","journal-title":"J Cleaner Prod"},{"key":"pone.0319530.ref010","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1186\/s40064-016-1869-2","article-title":"Formations of calcium carbonate minerals by bacteria and its multiple applications","volume":"5","author":"P Anbu","year":"2016","journal-title":"Springerplus"},{"issue":"2","key":"pone.0319530.ref011","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.ecoleng.2008.12.036","article-title":"Application of bacteria as self-healing agent for the development of sustainable concrete","volume":"36","author":"HM Jonkers","year":"2010","journal-title":"Ecol Eng"},{"key":"pone.0319530.ref012","doi-asserted-by":"crossref","first-page":"137993","DOI":"10.1016\/j.jclepro.2023.137993","article-title":"Microbial solutions to soil carbon sequestration","volume":"417","author":"ARG Mason","year":"2023","journal-title":"J Cleaner Prod"},{"key":"pone.0319530.ref013","doi-asserted-by":"crossref","first-page":"129610","DOI":"10.1016\/j.conbuildmat.2022.129610","article-title":"Carbon sequestration and utilization in cement-based materials and potential impacts on durability of structural concrete","volume":"361","author":"D Meng","year":"2022","journal-title":"Constr Build Mat"},{"issue":"5","key":"pone.0319530.ref014","doi-asserted-by":"crossref","first-page":"2797","DOI":"10.1007\/s10311-022-01458-1","article-title":"Algal biomass valorization for biofuel production and carbon sequestration: a review","volume":"20","author":"A Sarwer","year":"2022","journal-title":"Environ Chem Lett"},{"issue":"2","key":"pone.0319530.ref015","doi-asserted-by":"crossref","DOI":"10.1007\/s42452-020-1974-2","article-title":"Review of the use of microorganisms in geotechnical engineering applications","volume":"2","author":"KJ Osinubi","year":"2020","journal-title":"SN Appl Sci"},{"key":"pone.0319530.ref016","article-title":"Microbially induced calcium carbonate precipitation (MICP) and its potential in bioconcrete: microbiological and molecular concepts","volume":"6","author":"MJ Castro-Alonso","year":"2019","journal-title":"Front Mater"},{"key":"pone.0319530.ref017","doi-asserted-by":"crossref","first-page":"314","DOI":"10.3389\/fmicb.2013.00314","article-title":"Biomineralization of calcium carbonates and their engineered applications: a review","volume":"4","author":"NK Dhami","year":"2013","journal-title":"Front Microbiol"},{"key":"pone.0319530.ref018","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1016\/j.conbuildmat.2015.04.051","article-title":"A review of microbial precipitation for sustainable construction","volume":"93","author":"V Achal","year":"2015","journal-title":"Constr Build Mat"},{"issue":"2","key":"pone.0319530.ref019","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.ecoleng.2009.02.006","article-title":"Microbial carbonate precipitation in construction materials: a review","volume":"36","author":"W De Muynck","year":"2010","journal-title":"Ecol Eng"},{"issue":"2","key":"pone.0319530.ref020","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/s13146-017-0341-x","article-title":"Synthesis of precipitated calcium carbonate: a review","volume":"33","author":"OA Jimoh","year":"2017","journal-title":"Carbonates Evap"},{"key":"pone.0319530.ref021","volume-title":"BASF handbook: basics of coating technology","author":"HJ Streitberger","year":"2018","edition":"3"},{"key":"pone.0319530.ref022","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-0348-8245-3","volume-title":"Calcium carbonate: from the Cretaceous period into the 21st century","author":"FW Tegethoff","year":"2001"},{"key":"pone.0319530.ref023","volume-title":"Precipitated calcium carbonate market by end-user and geography - forecast and analysis 2020-2024","year":"2021"},{"key":"pone.0319530.ref024","doi-asserted-by":"crossref","first-page":"2954","DOI":"10.1016\/j.enconman.2005.02.009","article-title":"Production of precipitated calcium carbonate from calcium silicates and carbon dioxide","volume":"46","author":"S Teir","year":"2005","journal-title":"Energy Conv Manag"},{"issue":"1","key":"pone.0319530.ref025","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1080\/01490451.2022.2115169","article-title":"Techniques for quantifying bacterially induced carbonate mineralization in Escherichia coli","volume":"40","author":"GJ Breley","year":"2022","journal-title":"Geomicrobiol J"},{"issue":"5","key":"pone.0319530.ref026","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1080\/01490450903485136","article-title":"Bacterial calcium carbonate precipitation in cave environments: a function of calcium homeostasis","volume":"27","author":"ED Banks","year":"2010","journal-title":"Geomicrobiol J"},{"key":"pone.0319530.ref027","doi-asserted-by":"crossref","DOI":"10.1038\/msb4100050","article-title":"Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection","volume":"2","author":"T Baba","year":"2006","journal-title":"Mol Syst Biol"},{"issue":"5","key":"pone.0319530.ref028","doi-asserted-by":"crossref","DOI":"10.1128\/genomeA.01038-14","article-title":"Complete genome sequence of Escherichia coli BW25113","volume":"2","author":"F Grenier","year":"2014","journal-title":"Genome Announc"},{"issue":"5434","key":"pone.0319530.ref029","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1038\/246527a0","article-title":"Production of calcite (Calcium Carbonate) crystals by soil bacteria is a general phenomenon","volume":"246","author":"E Boquet","year":"1973","journal-title":"Nature"},{"issue":"11","key":"pone.0319530.ref030","doi-asserted-by":"crossref","first-page":"6856","DOI":"10.1128\/AEM.71.11.6856-6862.2005","article-title":"A propionate-inducible expression system for enteric bacteria","volume":"71","author":"SK Lee","year":"2005","journal-title":"Appl Environ Microbiol"},{"issue":"3","key":"pone.0319530.ref031","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1099\/mic.0.000604","article-title":"Loving the poison: the methylcitrate cycle and bacterial pathogenesis","volume":"164","author":"SK Dolan","year":"2018","journal-title":"Microbiology (Reading)"},{"issue":"3","key":"pone.0319530.ref032","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1073\/pnas.0337684100","article-title":"Engineering the metabolism of Escherichia coli W3110 for the conversion of sugar to redox-neutral and oxidized products: homoacetate production","volume":"100","author":"TB Causey","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"pone.0319530.ref033","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1074\/jbc.271.1.521","article-title":"Two hydrophobic subunits are essential for the heme b ligation and functional assembly of complex II (succinate-ubiquinone oxidoreductase) from Escherichia coli","volume":"271","author":"K Nakamura","year":"1996","journal-title":"J Biol Chem"},{"issue":"1","key":"pone.0319530.ref034","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.jbiotec.2011.03.015","article-title":"Characterization of E. coli MG1655 and frdA and sdhC mutants at various aerobiosis levels","volume":"154","author":"S Steinsiek","year":"2011","journal-title":"J Biotechnol"},{"key":"pone.0319530.ref035","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.3389\/fmicb.2018.01793","article-title":"Growth adaptation of gnd and sdhCB Escherichia coli deletion strains diverges from a similar initial perturbation of the transcriptome","volume":"9","author":"D McCloskey","year":"2018","journal-title":"Front Microbiol"},{"issue":"23","key":"pone.0319530.ref036","doi-asserted-by":"crossref","first-page":"7635","DOI":"10.1128\/jb.174.23.7635-7641.1992","article-title":"Carbon and energy metabolism of atp mutants of Escherichia coli","volume":"174","author":"PR Jensen","year":"1992","journal-title":"J Bacteriol"},{"issue":"19","key":"pone.0319530.ref037","doi-asserted-by":"crossref","first-page":"6869","DOI":"10.1128\/JB.00452-06","article-title":"Alterations of cellular physiology in Escherichia coli in response to oxidative phosphorylation impaired by defective F1-ATPase","volume":"188","author":"S Noda","year":"2006","journal-title":"J Bacteriol"},{"issue":"43","key":"pone.0319530.ref038","doi-asserted-by":"crossref","first-page":"27873","DOI":"10.1074\/jbc.273.43.27873","article-title":"Deletions in the second stalk of F1F0-ATP synthase in Escherichia coli","volume":"273","author":"PL Sorgen","year":"1998","journal-title":"J Biol Chem"},{"issue":"22","key":"pone.0319530.ref039","doi-asserted-by":"crossref","first-page":"6802","DOI":"10.1128\/jb.176.22.6802-6811.1994","article-title":"Bacillus subtilis F0F1 ATPase: DNA sequence of the atp operon and characterization of atp mutants","volume":"176","author":"M Santana","year":"1994","journal-title":"J Bacteriol"},{"issue":"3","key":"pone.0319530.ref040","first-page":"223","article-title":"The fermentation pathways of Escherichia coli","volume":"5","author":"DP Clark","year":"1989","journal-title":"FEMS Microbiol Rev"},{"issue":"2","key":"pone.0319530.ref041","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/BF00140485","article-title":"HPLC separation of enterobactin and linear 2,3-dihydroxybenzoylserine derivatives: a study on mutants of Escherichia coli defective in regulation (fur), esterase (fes) and transport (fepA)","volume":"7","author":"G Winkelmann","year":"1994","journal-title":"Biometals"},{"issue":"2","key":"pone.0319530.ref042","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1128\/jb.141.2.876-887.1980","article-title":"Sites of metal deposition in the cell wall of Bacillus subtilis","volume":"141","author":"TJ Beveridge","year":"1980","journal-title":"J Bacteriol"},{"issue":"16","key":"pone.0319530.ref043","doi-asserted-by":"crossref","first-page":"3319","DOI":"10.1016\/S0016-7037(97)00166-X","article-title":"A chemical equilibrium model for metal adsorption onto bacterial surfaces","volume":"61","author":"JB Fein","year":"1997","journal-title":"Geochimica et Cosmochimica Acta"},{"issue":"20","key":"pone.0319530.ref044","doi-asserted-by":"crossref","first-page":"5803","DOI":"10.1128\/JB.183.20.5803-5812.2001","article-title":"Analysis of a complete library of putative drug transporter genes in Escherichia coli","volume":"183","author":"K Nishino","year":"2001","journal-title":"J Bacteriol"},{"issue":"1","key":"pone.0319530.ref045","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1006\/jmbi.2000.3961","article-title":"Microbial genome analyses: comparative transport capabilities in eighteen prokaryotes","volume":"301","author":"IT Paulsen","year":"2000","journal-title":"J Mol Biol"},{"issue":"1","key":"pone.0319530.ref046","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1038\/s41467-017-01483-7","article-title":"The structural basis of proton driven zinc transport by ZntB","volume":"8","author":"C Gati","year":"2017","journal-title":"Nat Commun"},{"issue":"16","key":"pone.0319530.ref047","doi-asserted-by":"crossref","first-page":"4369","DOI":"10.1128\/JB.184.16.4369-4373.2002","article-title":"ZntB is a novel Zn2+ transporter in Salmonella enterica serovar Typhimurium","volume":"184","author":"AJ Worlock","year":"2002","journal-title":"J Bacteriol"},{"issue":"6","key":"pone.0319530.ref048","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1016\/j.bbamem.2008.02.006","article-title":"pH and monovalent cations regulate cytosolic free Ca(2+) in E. coli","volume":"1778","author":"R Naseem","year":"2008","journal-title":"Biochim Biophys Acta"},{"key":"pone.0319530.ref049","doi-asserted-by":"crossref","first-page":"1615","DOI":"10.3389\/fmicb.2017.01615","article-title":"Microbial carbonic anhydrases in biomimetic carbon sequestration for mitigating global warming: prospects and perspectives","volume":"8","author":"H Bose","year":"2017","journal-title":"Front Microbiol"},{"issue":"7","key":"pone.0319530.ref050","doi-asserted-by":"crossref","first-page":"15456","DOI":"10.3390\/ijms160715456","article-title":"Thermostable carbonic anhydrases in biotechnological applications","volume":"16","author":"A Di Fiore","year":"2015","journal-title":"Int J Mol Sci"},{"key":"pone.0319530.ref051","first-page":"6697","article-title":"Engineered Escherichia coli with periplasmic carbonic anhydrase as a biocatalyst for CO2 sequestration","author":"BH Jo","year":"2013","journal-title":"Appl Environ Microbiol"},{"issue":"7","key":"pone.0319530.ref052","doi-asserted-by":"crossref","first-page":"1865","DOI":"10.1002\/bit.24863","article-title":"Periplasmic expression of carbonic anhydrase in Escherichia coli: a new biocatalyst for CO(2) hydration","volume":"110","author":"TN Patel","year":"2013","journal-title":"Biotechnol Bioeng"},{"issue":"10","key":"pone.0319530.ref053","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1016\/j.chemosphere.2012.02.003","article-title":"Biomineralization-based conversion of carbon dioxide to calcium carbonate using recombinant carbonic anhydrase","volume":"87","author":"IG Kim","year":"2012","journal-title":"Chemosphere"},{"key":"pone.0319530.ref054","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1099\/00221287-148-1-133","article-title":"AcnC of Escherichia coli is a 2-methylcitrate dehydratase (PrpD) that can use citrate and isocitrate as substrates","volume":"148","author":"L Blank","year":"2002","journal-title":"Microbiology (Reading)"},{"issue":"24","key":"pone.0319530.ref055","doi-asserted-by":"crossref","first-page":"6184","DOI":"10.1046\/j.1432-1033.2002.03336.x","article-title":"Oxidation of propionate to pyruvate in Escherichia coli. Involvement of methylcitrate dehydratase and aconitase","volume":"269","author":"M Brock","year":"2002","journal-title":"Eur J Biochem"},{"key":"pone.0319530.ref056","doi-asserted-by":"crossref","first-page":"3877","DOI":"10.1099\/mic.0.27299-0","article-title":"2-Methylcitrate-dependent activation of the propionate catabolic operon (prpBCDE) of Salmonella enterica by the PrpR protein","volume":"150","author":"S Palacios","year":"2004","journal-title":"Microbiology (Reading)"},{"issue":"3","key":"pone.0319530.ref057","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/0304-4165(72)90014-1","article-title":"Genetic control of the metabolism of propionate by Escherichia coli K12","volume":"264","author":"WW Kay","year":"1972","journal-title":"Biochim Biophys Acta"},{"issue":"3","key":"pone.0319530.ref058","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.bbrc.2014.05.015","article-title":"Acs is essential for propionate utilization in Escherichia coli","volume":"449","author":"F Liu","year":"2014","journal-title":"Biochem Biophys Res Commun"},{"issue":"29","key":"pone.0319530.ref059","doi-asserted-by":"crossref","first-page":"19822","DOI":"10.1074\/jbc.M600333200","article-title":"Potassium\/proton antiport system of Escherichia coli","volume":"281","author":"MV Radchenko","year":"2006","journal-title":"J Biol Chem"},{"issue":"2","key":"pone.0319530.ref060","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.bbamem.2005.09.010","article-title":"Alkaline pH homeostasis in bacteria: new insights","volume":"1717","author":"E Padan","year":"2005","journal-title":"Biochim Biophys Acta"},{"issue":"11","key":"pone.0319530.ref061","doi-asserted-by":"crossref","first-page":"6856","DOI":"10.1128\/AEM.71.11.6856-6862.2005","article-title":"A propionate-inducible expression system for enteric bacteria","volume":"71","author":"SK Lee","year":"2005","journal-title":"Appl Environ Microbiol"},{"issue":"6","key":"pone.0319530.ref062","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1016\/j.bbabio.2011.10.008","article-title":"Disruption of individual nuo-genes leads to the formation of partially assembled NADH:ubiquinone oxidoreductase (complex I) in Escherichia coli","volume":"1817","author":"H Erhardt","year":"2012","journal-title":"Biochim Biophys Acta"},{"issue":"7","key":"pone.0319530.ref063","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1016\/j.bbabio.2016.01.012","article-title":"Structure of bacterial respiratory complex I","volume":"1857","author":"JM Berrisford","year":"2016","journal-title":"Biochimica et Biophysica Acta (BBA)-Bioenergetics"},{"issue":"1","key":"pone.0319530.ref064","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0087307","article-title":"Analysis of Escherichia coli mutants with a linear respiratory chain","volume":"9","author":"S Steinsiek","year":"2014","journal-title":"PLoS One"},{"issue":"3","key":"pone.0319530.ref065","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/S0005-2728(97)00034-0","article-title":"Alternative respiratory pathways of Escherichia coli: energetics and transcriptional regulation in response to electron acceptors","volume":"1320","author":"G Unden","year":"1997","journal-title":"Biochim Biophys Acta"},{"issue":"5","key":"pone.0319530.ref066","doi-asserted-by":"crossref","first-page":"3653","DOI":"10.1128\/AEM.72.5.3653-3661.2006","article-title":"Overflow metabolism in Escherichia coli during steady-state growth: transcriptional regulation and effect of the redox ratio","volume":"72","author":"GN Vemuri","year":"2006","journal-title":"Appl Environ Microbiol"},{"issue":"5","key":"pone.0319530.ref067","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1007\/s002530051433","article-title":"Glucose overflow metabolism and mixed-acid fermentation in aerobic large-scale fed-batch processes with Escherichia coli","volume":"51","author":"B Xu","year":"1999","journal-title":"Appl Microbiol Biotechnol"},{"issue":"5","key":"pone.0319530.ref068","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1080\/713851131","article-title":"Biomediated precipitation of calcium carbonate metastable phases in hypogean environments: a short review","volume":"20","author":"S Sanchez-Moral","year":"2003","journal-title":"Geomicrobiology Journal"},{"issue":"23","key":"pone.0319530.ref069","doi-asserted-by":"crossref","first-page":"9895","DOI":"10.1007\/s00253-016-7701-7","article-title":"Induced calcium carbonate precipitation using Bacillus species","volume":"100","author":"M Seifan","year":"2016","journal-title":"Appl Microbiol Biotechnol"},{"issue":"1","key":"pone.0319530.ref070","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1128\/JB.01450-06","article-title":"Bacillus subtilis gene cluster involved in calcium carbonate biomineralization","volume":"189","author":"C Barabesi","year":"2007","journal-title":"J Bacteriol"},{"issue":"3","key":"pone.0319530.ref071","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1306\/111302730485","article-title":"Bacterially induced mineralization of calcium carbonate in terrestrial environments: the role of exopolysaccharides and amino acids","volume":"73","author":"O Braissant","year":"2003","journal-title":"J Sediment Res"},{"issue":"1","key":"pone.0319530.ref072","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0210339","article-title":"Sporosarcina pasteurii can form nanoscale calcium carbonate crystals on cell surface","volume":"14","author":"T Ghosh","year":"2019","journal-title":"PLoS One"},{"key":"pone.0319530.ref073","volume-title":"Cave minerals of the world","author":"CA Hill","year":"1997"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0319530","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T18:26:32Z","timestamp":1765909592000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0319530"}},"subtitle":[],"editor":[{"given":"Sreenivasulu","family":"Basha","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,12,16]]},"references-count":73,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,12,16]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0319530","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,16]]}}}