{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,21]],"date-time":"2025-03-21T19:10:07Z","timestamp":1742584207426,"version":"3.40.2"},"reference-count":52,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2025,3,21]],"date-time":"2025-03-21T00:00:00Z","timestamp":1742515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Dha A, a biocatalyst with pronounced efficacy in the degradation of mustard gas, is constrained by its inherent instability, which impedes its broader application. In this study, we encapsulated Dha A within a poloxamer-based thermosensitive hydrogel, a widely utilized protein carrier, to assess its physicochemical characteristics, catalytic performance, and stability enhancement. The Dha A-loaded thermosensitive gel (Dha A@TSG) exhibited interactions between Dha A and poloxamer molecules via hydrogen bonding, with an optimal gelation temperature of 25\u00b0C. This encapsulation strategy significantly enhanced the solubility and catalytic efficiency of the mustard gas mimic, bis(2-chloroethyl) ether, surpassing the performance of the free Dha A solution. At 32\u00b0C, the poloxamer molecules within Dha A@TSG formed a tightly packed stereostucture, which substantially improved the storage and thermal stability of Dha A. Collectively, our findings offer valuable technical insights into the stabilization and catalytic efficiency enhancement of Dha A through the employment of poloxamer thermosensitive gels.<\/jats:p>","DOI":"10.1371\/journal.pone.0319810","type":"journal-article","created":{"date-parts":[[2025,3,21]],"date-time":"2025-03-21T18:28:29Z","timestamp":1742581709000},"page":"e0319810","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enhancing the stability and catalytic efficiency of alkyl halide dehalogenase through poloxamer temperature-sensitive gel"],"prefix":"10.1371","volume":"20","author":[{"given":"Jianjun","family":"Zhu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Gong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiong","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3439-8465","authenticated-orcid":true,"given":"Yu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,3,21]]},"reference":[{"issue":"5","key":"pone.0319810.ref001","doi-asserted-by":"crossref","first-page":"384","DOI":"10.3109\/10408444.2010.541224","article-title":"Sulfur mustard toxicity: history, chemistry, pharmacokinetics, and pharmacodynamics","volume":"41","author":"K Ghabili","year":"2011","journal-title":"Crit Rev Toxicol"},{"issue":"6","key":"pone.0319810.ref002","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1001\/archderm.1992.01680160059004","article-title":"Skin manifestations of mustard gas. 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