{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T12:58:03Z","timestamp":1785243483534,"version":"3.55.0"},"reference-count":27,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T00:00:00Z","timestamp":1748476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["grant no. 82060527"],"award-info":[{"award-number":["grant no. 82060527"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Science Foundation of the First Hospital of Lanzhou University","award":["no. ldyyyn2019-02"],"award-info":[{"award-number":["no. ldyyyn2019-02"]}]},{"DOI":"10.13039\/501100016321","name":"First Clinical Medical School, Lanzhou University","doi-asserted-by":"publisher","award":["no. lzuyxcx-2022-175"],"award-info":[{"award-number":["no. lzuyxcx-2022-175"]}],"id":[{"id":"10.13039\/501100016321","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"\n Objective<\/jats:title>\n To screen key enhancer RNAs (eRNAs) in thymoma (THYM) through The Cancer Genome Atlas (TCGA) database and explore their potential as prognostic molecules and therapeutic targets in THYM.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Gene expression RNA-seq profiles of 33 cancer types were retrieved and downloaded from the TCGA database, and Kaplan\u2013Meier survival analysis and Spearman correlation analysis were applied to screen eRNAs and target genes associated with survival in THYM patients. The correlation of target eRNAs with clinical features was assessed. Gene set enrichment analysis was performed to investigate the potential biological functions of target eRNAs. Finally, the prognostic potential of the eRNA was validated in other cancer types.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n In THYM,\n SATB1-AS1<\/jats:italic>\n and the target gene SATB1 high expression were associated with a positive patient prognosis, and\n SATB1-AS1<\/jats:italic>\n expression was negatively correlated with patient stage (\n P<\/jats:italic>\n \u2009<\u20090.05). Gene and pathway enrichment analyses revealed that\n SATB1-AS1<\/jats:italic>\n was associated with leukocyte transendothelial migration, natural killer cell-mediated cytotoxicity, neutrophil extracellular trap formation, PD-L1 expression and the PD-1 checkpoint pathway in cancer.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n SATB1-AS1<\/jats:italic>\n may be a key eRNA in THYM and has the potential to be a marker and therapeutic target for the early diagnosis and prognosis of THYM.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1371\/journal.pone.0319965","type":"journal-article","created":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T16:02:32Z","timestamp":1748534552000},"page":"e0319965","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Identification of the immune-associated enhancer RNA SATB1-AS1 as a novel biomarker for thymic cancer prognosis"],"prefix":"10.1371","volume":"20","author":[{"given":"Hongpeng","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yichu","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangtao","family":"Min","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3399-3755","authenticated-orcid":true,"given":"Lei","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,5,29]]},"reference":[{"issue":"1","key":"pone.0319965.ref001","first-page":"36","article-title":"Genome-wide enhancer RNA profiling adds molecular links between genetic variation and human cancers","volume":"11","author":"Y-M Cai","year":"2024","journal-title":"Mil Med 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