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The theory of <jats:italic>in silico<\/jats:italic> screening using TFs regulatory network analysis (ISNA) required the following requirements: (1) estimating promoter regions, (2) constructing TFs regulatory network (TRN) relationships using the nucleotide sequence information in the promoters and score matrices derived from TF consensus sequences, and (3) identifying candidate core TFs by determining dissociation constants (K<jats:sub>d<\/jats:sub> values) within the relationships of TRN. ISNA demonstrated the ability to predict the core TFs involved in the endothelial-to-mesenchymal transition of human umbilical vein endothelial cell (HUVEC) and the differentiation of human embryonic stem cells into mesodermal cells. Using ISNA, we identified HMGA2 as a novel core TF in uterine epithelium. Notably, HMGA2 expression was predominantly detected in uterine epithelium, where it regulated cell proliferation in response to estrogen. These findings highlight ISNA\u2019s potential to identify core TFs based on transcriptomic data.<\/jats:p>","DOI":"10.1371\/journal.pone.0319971","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T21:34:07Z","timestamp":1744061647000},"page":"e0319971","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["In silico screening system based on a transcription factors regulatory network only using transcriptomic 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