{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T08:44:31Z","timestamp":1767084271848,"version":"3.40.3"},"reference-count":25,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T00:00:00Z","timestamp":1744070400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82000982; 82192865; 81970875"],"award-info":[{"award-number":["82000982; 82192865; 81970875"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007162","name":"Guangdong Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["A2022078"],"award-info":[{"award-number":["A2022078"]}],"id":[{"id":"10.13039\/501100007162","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The clinical use of aminoglycosides often results in injury to vestibular hair cells and subsequent vestibular dysfunction. Thus, clarifying the targets and mechanisms underlying aminoglycoside-mediated damage is of urgent importance. Prostaglandin D2 synthase (<jats:italic>Ptgds<\/jats:italic>) is a glycoprotein that plays dual roles in lipid transport regulation and prostaglandin metabolism. However, the role of <jats:italic>Ptgds<\/jats:italic> in aminoglycoside-induced vestibular dysfunction remains unclear. This study aimed to explore the function of <jats:italic>Ptgds<\/jats:italic> in the utricle and HEI-OC1 cells. Neomycin injury induced high levels of <jats:italic>Ptgds<\/jats:italic> expression in utricle explants. Moreover, <jats:italic>Ptgds<\/jats:italic> knockdown protected against neomycin injury by enhancing cellular proliferation and viability while suppressing reactive oxygen species production, inflammation, and apoptosis. These findings suggest that <jats:italic>Ptgds<\/jats:italic> may serve as a novel therapeutic target for treating vestibular dysfunction caused by aminoglycoside-induced damage.<\/jats:p>","DOI":"10.1371\/journal.pone.0320634","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T17:25:35Z","timestamp":1744133135000},"page":"e0320634","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Ptgds downregulation protect vestibular hair cells from aminoglycoside-induced vestibulotoxicity"],"prefix":"10.1371","volume":"20","author":[{"given":"Chen","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhimin","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinghong","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7050-8094","authenticated-orcid":true,"given":"Guohui","family":"Nie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,4,8]]},"reference":[{"issue":"8","key":"pone.0320634.ref001","doi-asserted-by":"crossref","first-page":"2613","DOI":"10.1007\/s00204-021-03067-3","article-title":"Progress in protecting vestibular hair cells","volume":"95","author":"L Jiang","year":"2021","journal-title":"Arch Toxicol"},{"issue":"3","key":"pone.0320634.ref002","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1586\/14787210.2014.878648","article-title":"Aminoglycoside toxicity in neonates: Something to worry about?","volume":"12","author":"A Kent","year":"2014","journal-title":"Expert Rev Anti Infect Ther"},{"key":"pone.0320634.ref003","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1177\/00034894001090S504","article-title":"Temporal bone studies of the human peripheral vestibular system. 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