{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T21:50:31Z","timestamp":1793397031183,"version":"4.5.0"},"reference-count":40,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T00:00:00Z","timestamp":1743033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Kane Biotech Inc.","award":["2-21-23"],"award-info":[{"award-number":["2-21-23"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p><jats:italic>Cutibacterium acnes<\/jats:italic> plays a key role in the development of acne vulgaris, with biofilm formation contributing to its persistence and resistance to antimicrobial treatments. A critical component of <jats:italic>C. acnes<\/jats:italic> biofilms is poly-<jats:italic>N<\/jats:italic>-acetylglucosamine (PNAG), an exopolysaccharide that facilitates both biofilm stability and biocide resistance. This study evaluated the efficacy of the PNAG-degrading enzyme dispersin B in enhancing the susceptibility of <jats:italic>C. acnes<\/jats:italic> biofilms to benzoyl peroxide (BP), a common anti-acne agent. Dual-species biofilms of <jats:italic>C. acnes<\/jats:italic> and <jats:italic>Staphylococcus epidermidis<\/jats:italic>, which has been shown to promote <jats:italic>C. acnes<\/jats:italic> biofilm growth under aerobic conditions, were cultivated in\u00a0glass tubes and treated with dispersin B (5\u201380 \u00b5g\/mL), BP (0.1\u20132.5%), or a combination of both. Dispersin B or BP alone reduced <jats:italic>C. acnes<\/jats:italic> colony-forming units (CFUs) by 1\u20132 log units. However, sequential treatment with dispersin B followed by BP achieved a synergistic effect, yielding a &gt;6-log reduction in CFUs. Remarkably, concentrations as low as 5 \u00b5g\/mL dispersin B combined with 0.5% BP efficiently eradicated <jats:italic>C. acnes<\/jats:italic> from the dual-species biofilms. These findings highlight the protective role of PNAG against BP and demonstrate the potential of dispersin B as an adjunctive therapy to enhance the efficacy of BP in acne treatment.<\/jats:p>","DOI":"10.1371\/journal.pone.0320662","type":"journal-article","created":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T18:18:14Z","timestamp":1743099494000},"page":"e0320662","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Synergistic activity of dispersin B and benzoyl peroxide against Cutibacterium acnes\/Staphylococcus epidermidis dual-species biofilms"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1773-4372","authenticated-orcid":true,"given":"Jeffrey 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