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Dermatan 4-<jats:italic>O<\/jats:italic>-sulfotransferase 1 (D4ST1) is an enzyme that catalyzes the transfer of a sulfate group to the <jats:italic>N<\/jats:italic>-acetylgalactosamine residue of dermatan, resulting in mature DS. Biallelic loss-of-function variants in the carbohydrate sulfotransferase 14 (<jats:italic>CHST14<\/jats:italic>) gene encoding D4ST1, induce defective DS biosynthesis. DS deficiency causes severe connective tissue fragility and deformities in humans (musculocontractural Ehlers\u2013Danlos Syndrome [mcEDS]) and mice (<jats:italic>Chst14<\/jats:italic> gene knockout [<jats:italic>Chst14<\/jats:italic><jats:sup>-\/-<\/jats:sup>] mice). Many patients with mcEDS experience gastrointestinal symptoms such as constipation, diverticula, diverticulitis, and perforation. However, pathogenesis of these symptoms has not been systematically investigated. Therefore, we sought to determine the effects of DS deficiency on the colon using <jats:italic>Chst14<\/jats:italic><jats:sup>-\/-<\/jats:sup> mice. We found that collagen fibrils were abnormally arranged in the submucosa of the colon. The mice also exhibited accelerated colonic contraction. Unexpectedly, no significant aggravation of dextran sulfate sodium-induced colitis was observed in <jats:italic>Chst14<\/jats:italic><jats:sup>-\/-<\/jats:sup> mice compared with wild-type mice. These findings suggest a physiological role of DS in the colon and may shed light on the potential mechanisms underlying the gastrointestinal symptoms of mcEDS.<\/jats:p>","DOI":"10.1371\/journal.pone.0320943","type":"journal-article","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T17:25:26Z","timestamp":1746552326000},"page":"e0320943","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Carbohydrate sulfotransferase 14 gene deletion induces dermatan sulfate deficiency and affects collagen structure and bowel contraction"],"prefix":"10.1371","volume":"20","author":[{"given":"Fumiko","family":"Ono","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin","family":"Shimada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuji","family":"Mizumoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Miyata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Nitahara-Kasahara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1284-9795","authenticated-orcid":true,"given":"Shuhei","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Okada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoki","family":"Kosho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9938-6569","authenticated-orcid":true,"given":"Takahiro","family":"Yoshizawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,5,6]]},"reference":[{"issue":"13","key":"pone.0320943.ref001","doi-asserted-by":"crossref","DOI":"10.3390\/ijms23137485","article-title":"The Specific Role of Dermatan Sulfate as an Instructive Glycosaminoglycan in Tissue Development","volume":"23","author":"S Mizumoto","year":"2022","journal-title":"Int J Mol Sci"},{"key":"pone.0320943.ref002","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1146\/annurev.biochem.67.1.609","article-title":"Matrix proteoglycans: from molecular design to cellular function","volume":"67","author":"RV Iozzo","year":"1998","journal-title":"Annu Rev Biochem"},{"issue":"9","key":"pone.0320943.ref003","doi-asserted-by":"crossref","first-page":"117R-25R","DOI":"10.1093\/glycob\/cwf066","article-title":"Dermatan sulfate: new functions from an old glycosaminoglycan","volume":"12","author":"JM Trowbridge","year":"2002","journal-title":"Glycobiology"},{"key":"pone.0320943.ref004","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.sbi.2015.06.004","article-title":"Molecular interactions between chondroitin-dermatan sulfate and growth factors\/receptors\/matrix proteins","volume":"34","author":"S Mizumoto","year":"2015","journal-title":"Curr Opin Struct Biol"},{"issue":"3","key":"pone.0320943.ref005","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1042\/bj1980669","article-title":"Biosynthesis of dermatan sulphate. 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