{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T01:49:54Z","timestamp":1793756994658,"version":"4.6.1"},"reference-count":93,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T00:00:00Z","timestamp":1744761600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","award":["Researchers Supporting Project number (RSPD2025R676)"],"award-info":[{"award-number":["Researchers Supporting Project number (RSPD2025R676)"]}],"id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Gastric ulcer is a multifaceted ailment of multiple causes and is chronic warranting the discovery of remedies to alleviate its symptoms and severity. <jats:italic>Pancratium maritimum<\/jats:italic> L. is recognized for its several health benefits, although its potential against gastric ulcers has yet to be reported.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods and findings<\/jats:title>\n<jats:p>This study reports on the effects of <jats:italic>P. maritimum<\/jats:italic> L. whole plant (PM-EtOH) ethanol extract at a dose of 25, 50, and 100\u2009mg\/kg body weight orally for managing ethanol-induced peptic ulcer in rats. The anti-ulceration capacity of PM-EtOH was determined against ethanol (EtOH)-induced rats <jats:italic>via<\/jats:italic> biochemical, histological, immunohistochemical, and western blotting assays. The profiling of the bioactive metabolites in <jats:italic>P. maritimum<\/jats:italic> extract was based on Ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC-ESI-qTOF-MS\/MS) analysis. Following PM-EtOH treated group, the gastric glutathione (GSH) level dropped in the ulcer group receiving ethanol was restored to normal levels. Additionally, following PM-EtOH, elevated malondialdehyde (MDA) content in the stomach tissues diminished. PM-EtOH treated group displayed recovery and comparable morphology compared with normal group, concurrent with lower levels of Tumor Necrosis Factor \u03b1 (TNF-\u03b1), MyD88, and NLRP3, along with low expression of Nuclear Factor kappa <jats:italic>\u03b2<\/jats:italic> (NF-\u043a<jats:italic>\u03b2<\/jats:italic>) and high-mobility group box protein 1 (HMGB1) proteins. Immune-histochemicals of caspase-3 and toll-like receptors-4 (TLR-4) showed their normalization. These findings imply that PM-EtOH exerts a protective effect on rat stomach damage that has yet to be further tested in clinical trials for treatment of stomach ulcers. Phytochemical profiling of PM-EtOH <jats:italic>via<\/jats:italic> UHPLC-ESI-qTOF-MS\/MS led to the identification of 84 metabolites belonging to amino acids, organic acids, phenolic acids, alkaloids, flavonoids, and fatty acids to likely mediate for the observed effects.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>These outcomes provided evidence for the potential of PM-EtOH in gastric ulcers management.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0321018","type":"journal-article","created":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T17:53:01Z","timestamp":1744825981000},"page":"e0321018","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":16,"title":["In vivo anti-ulceration effect of Pancratium maritimum extract against ethanol-induced rats via NLRP3 inflammasome and HMGB1\/TLR4\/MYD88\/NF-\u03ba\u03b2 signaling pathways and its extract metabolite profile"],"prefix":"10.1371","volume":"20","author":[{"given":"Rehab F.","family":"Taher","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman M.","family":"Abd El ghany","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeinab A.","family":"El-Gendy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai M.","family":"Elghonemy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heba A.","family":"Hassan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gehad A.","family":"Abdel 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