{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T09:57:31Z","timestamp":1784023051570,"version":"3.55.0"},"reference-count":23,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T00:00:00Z","timestamp":1745971200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Knowledge Innovation Program of Wuhan -Basic Research","award":["2022020801010419"],"award-info":[{"award-number":["2022020801010419"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec055\">\n<jats:title>Importance<\/jats:title>\n<jats:p><jats:italic>Justicia procumbens<\/jats:italic> L. contains lignans with remarkable antiplatelet aggregation effects. Its ethyl acetate extract has demonstrated the efficacy of inhibiting platelet aggregation in preliminary tests. Identifying key components, elucidating the antithrombotic mechanism, and evaluating its potential for treating thrombotic diseases are of great significance.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec056\">\n<jats:title>Methods<\/jats:title>\n<jats:p>Chemical analysis was conducted on the ethyl acetate extract of <jats:italic>J. procumbens<\/jats:italic>. Animal models of carotid artery and venous thrombosis, combined with proteomics analysis, were employed to study the antithrombotic mechanism.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec057\">\n<jats:title>Results<\/jats:title>\n<jats:p>Chemical analysis identified justicidin B (JB) and chinensinaphthol methyl ether (CME) as the major active antithrombotic constituents. The extract inhibited platelet aggregation by regulating relevant signaling pathways, showing potential in preventing both venous and arterial thrombosis.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec058\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>This study confirmed that the ethyl acetate extract of <jats:italic>J. procumbens<\/jats:italic>, especially the lignan components JB and CME, can effectively inhibit platelet aggregation, showing promising prospects in treating thrombotic diseases. This provides ideas for the development of health products based on <jats:italic>J. procumbens<\/jats:italic> and lays a foundation for further clinical exploration.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0321023","type":"journal-article","created":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T18:01:34Z","timestamp":1746813694000},"page":"e0321023","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Antithrombotic effect and plasma pharmacochemistry of Justicia Procumbens 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burden of cardiovascular diseases and risk factors, 1990-2019: update from the GBD 2019 study","volume":"76","author":"GA Roth","year":"2020","journal-title":"J Am Coll Cardiol"},{"issue":"1","key":"pone.0321023.ref002","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1016\/j.clinthera.2023.09.027","article-title":"Safety and efficacy of direct oral anticoagulant in addition to antiplatelet therapy after acute coronary syndrome: a systemic review and meta-analysis of 53,869 patients","volume":"46","author":"A Alharbi","year":"2024","journal-title":"Clin Ther"},{"issue":"1","key":"pone.0321023.ref003","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.jep.2007.04.004","article-title":"Ethnobotanical survey of plants used to treat asthma in Andhra Pradesh, India","volume":"113","author":"N Savithramma","year":"2007","journal-title":"J Ethnopharmacol"},{"issue":"1-2","key":"pone.0321023.ref004","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0378-8741(00)00301-9","article-title":"Indigenous knowledge and uses of medicinal plants by local communities of the Kali Gandaki Watershed Area, Nepal","volume":"73","author":"AR Joshi","year":"2000","journal-title":"J Ethnopharmacol"},{"key":"pone.0321023.ref005","doi-asserted-by":"crossref","first-page":"681190","DOI":"10.3389\/fphar.2021.681190","article-title":"An ethnobotanical study of medicinal plants in Kinmen.Erratum in:","volume":"1213","author":"SS Huang","year":"2022","journal-title":"Front PharmacolFront Pharmacol"},{"issue":"6","key":"pone.0321023.ref006","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1002\/jssc.201000781","article-title":"Chromatographic fingerprint analysis and simultaneous determination of eight lignans in Justicia procumbens and its compound preparation by HPLC-DAD","volume":"34","author":"L Wang","year":"2011","journal-title":"J Sep Sci"},{"issue":"7","key":"pone.0321023.ref007","first-page":"860","article-title":"Comparative metabolite profiling of wild and cultivated Justicia procumbens L. based on 1H-NMR spectroscopy and HPLC-DAD analysis","volume":"9","author":"H Lee","year":"2020","journal-title":"Plants (Basel)"},{"issue":"5\u20136","key":"pone.0321023.ref008","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1163\/156856005774649313","article-title":"Anti-inflammatory activity of Justicia prostrata gamble in acute and sub-acute models of inflammation","volume":"13","author":"E Sanmugapriya","year":"2005","journal-title":"Inflammopharmacol"},{"issue":"12","key":"pone.0321023.ref009","first-page":"945","article-title":"Anti-HIV lignans from Justicia procumbens","volume":"17","author":"X-Y Xu","year":"2019","journal-title":"Chin J Nat Med"},{"issue":"13","key":"pone.0321023.ref010","doi-asserted-by":"crossref","first-page":"3185","DOI":"10.1016\/j.febslet.2006.04.085","article-title":"Caspase-8 acts as a key upstream executor of mitochondria during justicidin A-induced apoptosis in human hepatoma cells","volume":"580","author":"C-L Su","year":"2006","journal-title":"FEBS Lett"},{"issue":"2","key":"pone.0321023.ref011","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1208\/s12248-012-9449-z","article-title":"TargetHunter: an in silico target identification tool for predicting therapeutic potential of small organic molecules based on chemogenomic database","volume":"15","author":"L Wang","year":"2013","journal-title":"AAPS J"},{"issue":"D1","key":"pone.0321023.ref012","doi-asserted-by":"crossref","first-page":"D638","DOI":"10.1093\/nar\/gkac1000","article-title":"The STRING database in 2023: protein\u2013protein association networks and functional enrichment analyses for any sequenced genome of interest","volume":"51","author":"D Szklarczyk","year":"2023","journal-title":"Nucleic Acids Res"},{"issue":"11","key":"pone.0321023.ref013","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: a software environment for integrated models of biomolecular interaction networks","volume":"13","author":"P Shannon","year":"2003","journal-title":"Genome Res"},{"issue":"W1","key":"pone.0321023.ref014","doi-asserted-by":"crossref","first-page":"W216","DOI":"10.1093\/nar\/gkac194","article-title":"DAVID: a web server for functional enrichment analysis and functional annotation of gene lists (2021 update)","volume":"50","author":"BT Sherman","year":"2022","journal-title":"Nucleic Acids Research"},{"issue":"3","key":"pone.0321023.ref015","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1186\/scrt218","article-title":"Platelet-rich plasma preparation for regenerative medicine: optimization and quantification of cytokines and growth factors","volume":"4","author":"PR Amable","year":"2013","journal-title":"Stem Cell Res Ther"},{"issue":"12","key":"pone.0321023.ref016","doi-asserted-by":"crossref","first-page":"6265","DOI":"10.1021\/acs.jafc.3c08380","article-title":"Mudanpioside C discovered from paeonia suffruticosa andr. acts as a protein disulfide isomerase inhibitor with antithrombotic activities","volume":"72","author":"X Xie","year":"2024","journal-title":"J Agric Food Chem"},{"key":"pone.0321023.ref017","doi-asserted-by":"crossref","first-page":"110270","DOI":"10.1016\/j.biopha.2020.110270","article-title":"Inferior vena cava stenosis-induced deep vein thrombosis is influenced by multiple factors in rats","volume":"128","author":"H Liu","year":"2020","journal-title":"Biomed Pharmacother"},{"issue":"2","key":"pone.0321023.ref018","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/jcc.21334","article-title":"AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading","volume":"31","author":"O Trott","year":"2010","journal-title":"J Comput Chem"},{"issue":"11","key":"pone.0321023.ref019","doi-asserted-by":"crossref","first-page":"2622","DOI":"10.1016\/j.bmcl.2017.03.002","article-title":"Discovery of novel aminobenzisoxazole derivatives as orally available factor IXa inhibitors","volume":"27","author":"I Sakurada","year":"2017","journal-title":"Bioorg Med Chem Lett"},{"key":"pone.0321023.ref020","doi-asserted-by":"crossref","first-page":"115392","DOI":"10.1016\/j.jep.2022.115392","article-title":"Effective components and mechanism analysis of anti-platelet aggregation effect of Justicia procumbens L","volume":"294","author":"B Liu","year":"2022","journal-title":"J Ethnopharmacol"},{"issue":"1","key":"pone.0321023.ref021","doi-asserted-by":"crossref","first-page":"17187","DOI":"10.1038\/s41598-021-96683-z","article-title":"Reveals of candidate active ingredients in Justicia and its anti-thrombotic action of 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Nees","volume":"34","author":"S Wu","year":"2019","journal-title":"J Enzyme Inhib Med Chem"},{"key":"pone.0321023.ref023","doi-asserted-by":"crossref","first-page":"688","DOI":"10.3389\/fphar.2019.00688","article-title":"A novel antiplatelet aggregation target of justicidin b obtained from Rostellularia Procumbens (L.) 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