{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T03:47:04Z","timestamp":1791776824053,"version":"4.3.4"},"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T00:00:00Z","timestamp":1748908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004775","name":"Natural Science Foundation of Gansu Province","doi-asserted-by":"publisher","award":["22JR5RA716"],"award-info":[{"award-number":["22JR5RA716"]}],"id":[{"id":"10.13039\/501100004775","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Gansu Provincial Science and Technology Major Special Program Project","award":["22ZD6FA034"],"award-info":[{"award-number":["22ZD6FA034"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p><jats:italic>Mycoplasma pneumoniae<\/jats:italic> is a common cause of pneumonia in children over 5 years of age. Cell Population Data (CPD) is valuable for screening various diseases, including certain infections and myeloproliferative disorders, but less used in the diagnosis of children <jats:italic>mycoplasma pneumoniae<\/jats:italic> pneumonia (MPP).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Aims<\/jats:title>\n<jats:p>To improve the diagnostic efficacy and severity assessment of MPP in children by combining CPD with inflammatory markers.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Methods<\/jats:title>\n<jats:p>This study was conducted at the Gansu Provincial Maternity and Child-care Hospital, China. The data was accessed on 01.10.2024 for the purpose of research. A retrospective study from 01.10. 2022\u201301.01.2024 included three groups of children aged 5\u20139 years: 1) those with MPP (n\u2009=\u2009240), of which 73 were mild, 167 were severe; 2) those with bacterial pneumonia (n\u2009=\u200952); and 3) those with health check-ups (n\u2009=\u2009340). CPD indicators were measured by Sysmex XN-9000 (Kobe, Japan) hematology analyzer. Inflammatory markers included PCT (procalcitonin), LDH (lactate dehydrogenase), CK-MB (creatine kinase isoenzyme), CRP (c-reactive protein), SAA (serum amyloid A), APTT (activated partial thromboplastin time), FIB (fibrinogen). The differences and correlation of parameters between three groups were analyzed and receiver operating characteristic (ROC) analysis was performed.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Results<\/jats:title>\n<jats:p>Most of the leukocyte parameter indices showed statistically significant differences between the MPP group and the healthy physical examination group (<jats:italic>p\u2009&lt;\u20090.05<\/jats:italic>). Compared with bacterial pneumonia group, inflammatory markers were not correlated with the occurrence of MPP (<jats:italic>p\u2009&gt;\u20090.05<\/jats:italic>), while LY-X and NE-WY were negatively correlated with the occurrence of MPP (<jats:italic>p\u2009&lt;\u20090.05<\/jats:italic>). ROC analysis showed that the areas under the curve (AUC) for LY-X and NE-WY were 0.707 and 0.736, respectively. Additionally, LY-WY and MO-WZ are positively correlated with the severity of MPP (<jats:italic>p\u2009&lt;\u20090.05<\/jats:italic>), and the AUC for MO-WZ was 0.709. Among the inflammatory markers, the AUC for SAA was 0.828, and combining SAA with MO-WZ increased the AUC to 0.861 in distinguishing mild from severe MPP.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec005\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Peripheral blood leukocyte parameters may have clinical application value for the early diagnosis and disease progression assessment of MPP.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0321454","type":"journal-article","created":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T17:38:59Z","timestamp":1748972339000},"page":"e0321454","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Use peripheral blood leukocyte parameters combined with inflammatory indicators in diagnosis and severity assessment of mycoplasma pneumoniae pneumonia in 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vitro susceptibilities to and bactericidal activities of garenoxacin (BMS-284756) and other antimicrobial agents against human mycoplasmas and ureaplasmas","volume":"47","author":"KB Waites","year":"2003","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0321454.ref002","article-title":"British Thoracic Society guidelines for the management of community acquired pneumonia in children: update 2011","author":"M Harris","year":"2011","journal-title":"Thorax"},{"issue":"6","key":"pone.0321454.ref003","article-title":"Resurgence of Mycoplasma pneumonia by macrolide-resistant epidemic clones in China","volume":"5","author":"H Li","year":"2024","journal-title":"Lancet Microbe"},{"key":"pone.0321454.ref004","first-page":"291","volume-title":"Guidelines for the Diagnosis and Treatment of Mycoplasma Pneumoniae Pneumonia in Children (2023 Edition)","author":"S Zhao","year":"2023"},{"issue":"3","key":"pone.0321454.ref005","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1128\/CMR.00114-16","article-title":"Mycoplasma pneumoniae from the respiratory tract and beyond","volume":"30","author":"KB Waites","year":"2017","journal-title":"Clin Microbiol Rev"},{"issue":"10","key":"pone.0321454.ref006","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1001\/archpedi.156.10.1005","article-title":"Use of simple heuristics to target macrolide prescription in children with community-acquired pneumonia.","volume":"156","author":"JE Fischer","year":"2002","journal-title":"Arch Pediatr Adolesc Med"},{"issue":"5","key":"pone.0321454.ref007","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pmed.1001444","article-title":"Carriage of Mycoplasma pneumoniae in the upper respiratory tract of symptomatic and asymptomatic children: an observational study","volume":"10","author":"EB Spuesens","year":"2013","journal-title":"PLoS 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Chhabra","year":"2022","journal-title":"Int J Lab Hematol"},{"issue":"3","key":"pone.0321454.ref014","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1111\/ijlh.12790","article-title":"Abnormal scattergrams and cell population data generated by fully automated hematological analyzers: new tools for screening malaria infection?","volume":"40","author":"S Buoro","year":"2018","journal-title":"Int J Lab Hematol"},{"issue":"3","key":"pone.0321454.ref015","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1007\/s12288-020-01276-x","article-title":"Application of sysmex XN-series automated haematology analyser in the rapid detection of malaria","volume":"36","author":"A Ningombam","year":"2020","journal-title":"Indian J Hematol Blood Transfus"},{"issue":"6","key":"pone.0321454.ref016","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1515\/cclm-2018-0979","article-title":"Improvement in detecting sepsis using leukocyte cell population data (CPD","volume":"57","author":"E Urrechaga","year":"2019","journal-title":"Clin Chem Labor Med"},{"issue":"3","key":"pone.0321454.ref017","first-page":"851","article-title":"Evaluation of Sysmex XN-3000 for detection of schistocyte","volume":"30","author":"D-F Hou","year":"2022","journal-title":"Zhongguo Shi Yan Xue Ye Xue Za Zhi"},{"issue":"4","key":"pone.0321454.ref018","doi-asserted-by":"crossref","DOI":"10.1111\/ijlh.13779","article-title":"Cell population data from Sysmex XN analyzer and myelodysplastic syndrome","volume":"44","author":"PT Murphy","year":"2022","journal-title":"Int J Lab Hematol"},{"issue":"6","key":"pone.0321454.ref019","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1111\/j.1574-6976.2008.00129.x","article-title":"Epidemiology, clinical manifestations, pathogenesis and laboratory detection of Mycoplasma pneumoniae infections","volume":"32","author":"TP Atkinson","year":"2008","journal-title":"FEMS Microbiol 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Characteristics of laboratory examination indicators of mycoplasma pneumonia in children. 2021;7(09):106\u20139."},{"issue":"1","key":"pone.0321454.ref025","article-title":"Diagnostic value of serum ELISA of Mycoplasma pneumoniae IgM and IgG and passive particle agglutination for Mycoplasma pneumoniae pneumonia in children","volume":"67","author":"M Ge","year":"2021","journal-title":"Clin Lab"},{"issue":"3","key":"pone.0321454.ref026","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1189\/jlb.0711387","article-title":"Myeloperoxidase associated with neutrophil extracellular traps is active and mediates bacterial killing in the presence of hydrogen peroxide","volume":"91","author":"H Parker","year":"2012","journal-title":"J Leukoc Biol"},{"issue":"4","key":"pone.0321454.ref027","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/cdd.2011.1","article-title":"Dying for a cause: NETosis, mechanisms behind an antimicrobial cell death modality","volume":"18","author":"Q Remijsen","year":"2011","journal-title":"Cell Death Differ"},{"issue":"1","key":"pone.0321454.ref028","doi-asserted-by":"crossref","first-page":"13445","DOI":"10.1038\/s41598-019-49937-w","article-title":"Increased granulopoiesis in the bone marrow following Epstein-Barr virus infection","volume":"9","author":"Y Katahira","year":"2019","journal-title":"Sci Rep"},{"key":"pone.0321454.ref029","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1146\/annurev-pathol-020712-164023","article-title":"The multifaceted functions of neutrophils","volume":"9","author":"TN Mayadas","year":"2014","journal-title":"Annu Rev Pathol"},{"issue":"3","key":"pone.0321454.ref030","doi-asserted-by":"crossref","first-page":"967","DOI":"10.3892\/etm.2021.10399","article-title":"Thrombosis associated with Mycoplasma pneumoniae infection (Review)","volume":"22","author":"J Liu","year":"2021","journal-title":"Exp Ther Med"},{"issue":"24","key":"pone.0321454.ref031","first-page":"3954","article-title":"Clinical analysis of severe mycoplasma pneumoniae pneumonia with pulmonary embolism in children","volume":"21","author":"W Wang","year":"2021","journal-title":"Chin Drug Clin"},{"key":"pone.0321454.ref032","first-page":"82016","article-title":"Analysis of high risk factors of severe mycoplasma pneumoniae pneumonia in children","author":"L Xiaoyuan Min","journal-title":"Shanghai Med J"},{"key":"pone.0321454.ref033","unstructured":"Zhao J Clinical characteristics and high risk factors of severe mycoplasma pneumoniae pneumonia with extrapulmonary complications. 2021."},{"issue":"7","key":"pone.0321454.ref034","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1111\/crj.13620","article-title":"Clinical characteristics and serum inflammatory markers of community-acquired mycoplasma pneumonia in children","volume":"17","author":"F Fan","year":"2023","journal-title":"Clin Respir J"},{"issue":"6","key":"pone.0321454.ref035","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1002\/jgf2.201","article-title":"Clinical features and seasonal variations in the prevalence of macrolide-resistant Mycoplasma pneumoniae","volume":"19","author":"Y Akashi","year":"2018","journal-title":"J Gen Fam Med"},{"issue":"1","key":"pone.0321454.ref036","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1080\/00365513.2020.1858491","article-title":"High ratio of C-reactive protein\/procalcitonin predicts Mycoplasma pneumoniae infection among adults hospitalized with community acquired pneumonia","volume":"81","author":"F Li","year":"2021","journal-title":"Scand J Clin Lab Invest"},{"issue":"10","key":"pone.0321454.ref037","doi-asserted-by":"crossref","first-page":"1638","DOI":"10.1515\/cclm-2019-0194","article-title":"A high C-reactive protein\/procalcitonin ratio predicts Mycoplasma pneumoniae infection","volume":"57","author":"OL Neeser","year":"2019","journal-title":"Clin Chem Lab Med"},{"key":"pone.0321454.ref038","doi-asserted-by":"crossref","first-page":"828437","DOI":"10.3389\/fped.2022.828437","article-title":"D-dimer: the risk factor of children\u2019s severe Mycoplasma pneumoniae pneumonia","volume":"10","author":"J Qiu","year":"2022","journal-title":"Front Pediatr"},{"key":"pone.0321454.ref039","doi-asserted-by":"crossref","first-page":"687391","DOI":"10.3389\/fcimb.2021.687391","article-title":"The level of D-dimer is positively correlated with the severity of Mycoplasma pneumoniae pneumonia in children","volume":"11","author":"Y Zheng","year":"2021","journal-title":"Front Cell Infect Microbiol"},{"issue":"10","key":"pone.0321454.ref040","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.4187\/respcare.03920","article-title":"Lactate dehydrogenase as a biomarker for prediction of refractory Mycoplasma pneumoniae pneumonia in children","volume":"60","author":"A 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