{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T19:22:09Z","timestamp":1778181729870,"version":"3.51.4"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T00:00:00Z","timestamp":1744761600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["823015751003724"],"award-info":[{"award-number":["823015751003724"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec>\n<jats:title>Background<\/jats:title>\n<jats:p>Existing studies have focused on the relationship between vitamin B6 and thyroid disease. However, there is a lack of large cross-sectional studies reporting on the relationship between vitamin B6 and thyroid function. Therefore, the present study aimed to assess the association between vitamin B6 intake and thyroid function in a population of US adults aged 20 years and older, using data from the National Health and Nutrition Examination Survey (NHANES) between 2007 and 2012.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>Demographic, dietary, thyroid function, and relevant data from NHANES 2007\u20132012 were collected. The relationship between vitamin B6 intake and thyroid function was analysed using weighted multiple regression and restricted cubic spline analysis, including subgroup and interaction analysis.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>The study included 6954 participants with a weighted mean age of 47.39 \u2009\u00b1\u200916.60 years, mean vitamin B6 intake of 2.07\u2009\u00b1\u20091.15\u2009mg, and mean TT4 level of 7.88\u2009\u00b1\u20091.61 \u03bcg\/dL. A statistically significant negative correlation was noted between vitamin B6 intake and TT4 levels (\u03b2\u2009\u2009=\u2009\u2009\u22120.05, 95% CI\u2009\u2009=\u2009\u2009\u22120.10 to 0.00, P\u2009\u2009=\u2009\u20090.033). In addition to this, subgroup analyses we found: In the gender subgroup, a significant negative correlation was found between vitamin B6 intake and TT4 levels in the male population (\u03b2\u2009=\u2009\u22120.06, 95% CI\u2009\u2009=\u2009\u2009\u22120.11 to \u22120.01, P\u2009\u2009=\u2009\u20090.028); in the age subgroup, a significant negative correlation was found between vitamin B6 intake and TT4 levels in older people aged 60\u201380 years (\u03b2\u2009\u2009=\u2009\u2009\u22120. 13, 95% CI\u2009\u2009=\u2009\u2009\u22120.23 to \u22120.04, P\u2009\u2009=\u2009\u20090.008); in the BMI subgroup, we found a significant negative correlation between vitamin B6 intake and TT4 levels in overweight people (BMI: 25\u201329.9\u2009kg\/m<jats:sup>2<\/jats:sup>) (\u03b2\u2009\u2009=\u2009\u2009\u22120.13, 95% CI\u2009\u2009=\u2009\u2009\u22120.20 to \u22120.06, P\u2009&lt;\u20090. 001); in the iodine content subgroup, we found a significant negative correlation between vitamin B6 intake and TT4 levels in people with a normal iodine intake (100\u2013299 ug\/L) (\u03b2\u2009\u2009=\u2009\u2009\u22120.07, 95% CI\u2009\u2009=\u2009\u2009\u22120.13 to \u22120.01, P\u2009\u2009=\u2009\u20090.021). Finally, in the subgroups of gender, age, BMI, and iodine content, no interaction was found.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>To summarise, we believe that vitamin B6 may reduce serum TT4 levels by inhibiting inflammation.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0321688","type":"journal-article","created":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T17:40:43Z","timestamp":1744825243000},"page":"e0321688","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Relationship between vitamin B6 intake and thyroid function in US adults: NHANES 2007\u20132012 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Treuting","year":"2017"},{"issue":"1","key":"pone.0321688.ref012","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.beem.2009.08.010","article-title":"Iodine excess","volume":"24","author":"H B\u00fcrgi","year":"2010","journal-title":"Best Pract Res Clin Endocrinol Metab"},{"issue":"10","key":"pone.0321688.ref013","doi-asserted-by":"crossref","first-page":"3330","DOI":"10.3390\/nu13103330","article-title":"the association between the dietary inflammatory index and thyroid function in U.S. adult males","volume":"13","author":"N Liu","year":"2021","journal-title":"Nutrients"},{"issue":"7","key":"pone.0321688.ref014","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1007\/s40618-022-01987-0","article-title":"The association between dietary selenium intake and Hashimoto\u2019s thyroiditis among US adults: National Health and Nutrition Examination Survey (NHANES), 2007\u20132012","volume":"46","author":"G Zheng","year":"2023","journal-title":"J Endocrinol.\u00a0Invest"},{"issue":"5","key":"pone.0321688.ref015","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1055\/s-2007-979169","article-title":"Influence of zinc and selenium deficiency on parameters relating to thyroid hormone metabolism","volume":"28","author":"A Kralik","year":"1996","journal-title":"Horm Metab Res"},{"issue":"4","key":"pone.0321688.ref016","doi-asserted-by":"crossref","first-page":"3586","DOI":"10.3390\/ijms24043586","article-title":"Vitamin D and the thyroid: a critical review of the current evidence","volume":"24","author":"M Babi\u0107 Leko","year":"2023","journal-title":"Int J Mol Sci"},{"issue":"8","key":"pone.0321688.ref017","doi-asserted-by":"crossref","first-page":"3603","DOI":"10.1007\/s12011-022-03455-5","article-title":"Relationships Between Maternal Selected Metals (Cu, Mg, Zn and Fe), thyroid function and blood glucose levels during pregnancy","volume":"201","author":"W Zhang","year":"2023","journal-title":"Biol Trace Elem Res"},{"issue":"5","key":"pone.0321688.ref018","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1210\/jcem-45-5-1019","article-title":"Suppression of thyrotropin (TSH) and prolactin (PRL) release by pyridoxine in chronic primary hypothyroidism","volume":"45","author":"G Delitala","year":"1977","journal-title":"J Clin Endocrinol Metab"},{"key":"pone.0321688.ref019","first-page":"1","article-title":"Measurement of serum vitamins B2 and B6 in patients with Hashimoto\u2019s thyroiditis by LC\u2013MS\/MS method","author":"AA Mikulska-Sauermann","year":"2024","journal-title":"Chromatographia"},{"key":"pone.0321688.ref020","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1111\/j.1749-6632.1990.tb28049.x","article-title":"Neurobiology of pyridoxine","volume":"585","author":"K Dakshinamurti","year":"1990","journal-title":"Ann N Y Acad 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Nutr"},{"issue":"1","key":"pone.0321688.ref024","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s12937-023-00866-5","article-title":"Relationship between caffeine intake and thyroid function: results from NHANES 2007-2012","volume":"22","author":"J Zheng","year":"2023","journal-title":"Nutr J"},{"key":"pone.0321688.ref025","doi-asserted-by":"crossref","first-page":"1002489","DOI":"10.3389\/fnut.2022.1002489","article-title":"Relationship between dietary selenium intake and serum thyroid function measures in U.S. adults: Data from NHANES 2007-2012","volume":"9","author":"F Liu","year":"2022","journal-title":"Front Nutr"},{"issue":"4","key":"pone.0321688.ref026","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1017\/S000711451400395X","article-title":"Associations between dietary inflammatory index and inflammatory markers in the Asklepios Study","volume":"113","author":"N Shivappa","year":"2015","journal-title":"Br J Nutr"},{"issue":"4","key":"pone.0321688.ref027","doi-asserted-by":"crossref","first-page":"10.1002\/mnfr.201600630","DOI":"10.1002\/mnfr.201600630","article-title":"Association between the dietary inflammatory index (DII) and telomere length and C-reactive protein from the National Health and Nutrition Examination Survey-1999-2002","volume":"61","author":"N Shivappa","year":"2017","journal-title":"Mol Nutr Food Res"},{"issue":"1","key":"pone.0321688.ref028","doi-asserted-by":"crossref","first-page":"1305386","DOI":"10.3389\/fendo.2023.1305386","article-title":"Correlation between systemic inflammatory response index and thyroid function: 2009-2012 NHANES results","volume":"14","author":"Y Zhai","year":"2024","journal-title":"Front.\u00a0Endocrinol"},{"key":"pone.0321688.ref029","volume-title":"Thyroid hormone synthesis and secretion","author":"B Rousset","year":"2015"},{"issue":"1","key":"pone.0321688.ref030","doi-asserted-by":"crossref","first-page":"103","DOI":"10.3945\/jn.109.114397","article-title":"Vitamin B-6 intake is inversely related to, and the requirement is affected by, inflammation status","volume":"140","author":"MS Morris","year":"2010","journal-title":"J Nutr"},{"issue":"2","key":"pone.0321688.ref031","doi-asserted-by":"crossref","first-page":"337","DOI":"10.3945\/ajcn.2009.28571","article-title":"Association of vitamin B-6 status with inflammation, oxidative stress, and chronic inflammatory conditions: the Boston Puerto Rican Health Study","volume":"91","author":"J Shen","year":"2010","journal-title":"Am J Clin Nutr"},{"issue":"1","key":"pone.0321688.ref032","doi-asserted-by":"crossref","first-page":"132","DOI":"10.3945\/an.113.005207","article-title":"Vitamin B-6","volume":"6","author":"PJ Stover","year":"2015","journal-title":"Adv Nutr"},{"issue":"4","key":"pone.0321688.ref033","doi-asserted-by":"crossref","first-page":"863","DOI":"10.3945\/ajcn.113.072025","article-title":"Kynurenine pathway metabolites: relevant to vitamin B-6 deficiency and beyond","volume":"98","author":"MA Ciorba","year":"2013","journal-title":"Am J Clin Nutr"},{"key":"pone.0321688.ref034","doi-asserted-by":"crossref","DOI":"10.1016\/j.tips.2023.04.006","volume-title":"Modulation of T cells by tryptophan metabolites in the kynurenine pathway","author":"TW Stone","year":"2023"},{"key":"pone.0321688.ref035","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.mehy.2018.06.021","article-title":"Hypothesis kynurenic and quinolinic acids: The main players of the kynurenine pathway and opponents in inflammatory disease","volume":"118","author":"AA-B Badawy","year":"2018","journal-title":"Med Hypotheses"},{"key":"pone.0321688.ref036","doi-asserted-by":"crossref","first-page":"2570","DOI":"10.3389\/fimmu.2019.02570","article-title":"Immunomodulatory effects of genetic alterations affecting the kynurenine pathway","volume":"10","author":"FA Boros","year":"2019","journal-title":"Front Immunol"},{"issue":"1","key":"pone.0321688.ref037","doi-asserted-by":"crossref","first-page":"6851","DOI":"10.1038\/s41598-024-57154-3","article-title":"Alteration in kynurenine pathway metabolites in young women with autoimmune thyroiditis","volume":"14","author":"A Krupa","year":"2024","journal-title":"Sci Rep"},{"issue":"11","key":"pone.0321688.ref038","doi-asserted-by":"crossref","first-page":"2913","DOI":"10.3390\/molecules23112913","article-title":"Design, synthesis, molecular modeling, and biological evaluation of novel thiouracil derivatives as potential antithyroid agents","volume":"23","author":"SM Awad","year":"2018","journal-title":"Molecules"},{"issue":"12","key":"pone.0321688.ref039","doi-asserted-by":"crossref","first-page":"1670","DOI":"10.1089\/thy.2014.0028","article-title":"Guidelines for the treatment of hypothyroidism: prepared by the american thyroid association task force on thyroid hormone replacement","volume":"24","author":"J Jonklaas","year":"2014","journal-title":"Thyroid"},{"issue":"6","key":"pone.0321688.ref040","doi-asserted-by":"crossref","first-page":"e046607","DOI":"10.1136\/bmjopen-2020-046607","article-title":"Birth control pills and risk of hypothyroidism: a cross-sectional study of the National Health and Nutrition Examination Survey, 2007\u20132012","volume":"11","author":"Y Qiu","year":"2021","journal-title":"BMJ open"},{"issue":"1","key":"pone.0321688.ref041","doi-asserted-by":"crossref","first-page":"15646","DOI":"10.1038\/s41598-020-72725-w","article-title":"Thyroid redox imbalance in adult Wistar rats that were exposed to nicotine during breastfeeding","volume":"10","author":"RA Miranda","year":"2020","journal-title":"Sci Rep"},{"issue":"2","key":"pone.0321688.ref042","doi-asserted-by":"crossref","first-page":"R31-40","DOI":"10.1530\/JOE-13-0588","article-title":"Sexual dimorphism and thyroid dysfunction: a matter of oxidative stress?","volume":"221","author":"RS Fortunato","year":"2014","journal-title":"J Endocrinol"},{"issue":"11","key":"pone.0321688.ref043","doi-asserted-by":"crossref","first-page":"e13260","DOI":"10.1111\/acel.13260","article-title":"Thyroid hormones in diabetes, cancer, and aging","volume":"19","author":"BR Gauthier","year":"2020","journal-title":"Aging Cell"},{"issue":"3","key":"pone.0321688.ref044","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1530\/EJE-09-0441","article-title":"Free thyroxine is an independent predictor of subcutaneous fat in euthyroid individuals","volume":"161","author":"M Alevizaki","year":"2009","journal-title":"Eur J 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