{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T21:45:47Z","timestamp":1785447947841,"version":"3.56.0"},"reference-count":64,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T00:00:00Z","timestamp":1745884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Intestinal parasitic infections in Sri Lanka have received little attention due to their perceived low prevalence. Consuming raw vegetables without proper washing is one of the main ways to transmit intestinal parasitic infections. Therefore, this study investigated the contamination of parasites in vegetables. A cross-sectional study was conducted from August 2023 to January 2024 at fifty selected open markets in ten cities (Ragama, Miriswatta, Kirillawala, Kadawatha, Balummahara, Kiribathgoda, Peliyagoda, Weliweriya, Imbulgoda and Gampaha) in Gampaha District, Sri Lanka. Four vegetable types, namely <jats:italic>Centella asiatica<\/jats:italic> (Gatu kola) [n\u2009=\u2009162], <jats:italic>Ipomoea aquatic<\/jats:italic>a (Kankun) [n\u2009=\u2009150], <jats:italic>Alternanthera sessilis<\/jats:italic> (Mukunuwenna) [n\u2009=\u2009160] and <jats:italic>Lactuca sativa<\/jats:italic> (Lettuce) [n\u2009=\u2009148] that are consumed either raw or half cooked were selected. Approximately 50\u2009g of each vegetable was taken randomly at each sampling attempt. Each sample was dipped in the Tween 20 (5%) in a shaker for 15 minutes, followed by sedimentation overnight and centrifugation (2000\u2009\u00d7\u2009g for 15\u2009min). Both supernatant and sediment were examined under a light microscope to detect parasitic stages. The overall prevalence of parasitic contamination in the samples was 21.29% (n\u2009=\u2009132). <jats:italic>Centella asiatica<\/jats:italic> indicated the highest contamination (37.65% [61\/162]). <jats:italic>Lactuca sativa<\/jats:italic> had the lowest contamination (2.02% [3\/148]). Parasites including protozoan (<jats:italic>Entamoeba histolytica, Giardia lamblia, Toxoplasma gondii, Blastocystis hominis<\/jats:italic>, <jats:italic>Paramphistomum<\/jats:italic> spp and <jats:italic>Balantidium coli<\/jats:italic>) and helminths (<jats:italic>Ascaris<\/jats:italic> spp<jats:italic>., Hymenolepis<\/jats:italic> spp<jats:italic>., Strongyloides stercoralis, Trichuris<\/jats:italic> spp, <jats:italic>Taenia<\/jats:italic> spp, <jats:italic>Toxocara canis<\/jats:italic> and hookworms) were detected. <jats:italic>Blastocystis hominis<\/jats:italic> was the most predominant (4.68%; n\u2009=\u200929). <jats:italic>Toxocara<\/jats:italic> sp, <jats:italic>G. lamblia<\/jats:italic> and <jats:italic>Eimeria<\/jats:italic> sp were identified as least abundant (0.77%; n\u2009=\u20095). Every sample contained at least one parasitic contamination. A significant difference between the type of vegetables and the existence of parasites was identified (<jats:italic>P<\/jats:italic>\u2009=\u20090.008). The parasitic stages identified could cause infections among people with poor food hygienic\/ preparation practices. Therefore, health authorities must educate consumers on precautionary measures to prevent re-emerging intestinal parasitic infections in Sri Lanka.<\/jats:p>","DOI":"10.1371\/journal.pone.0321853","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T17:52:04Z","timestamp":1745949124000},"page":"e0321853","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Parasitic contamination in green vegetables from open markets in Gampaha District, Sri Lanka: Implications for human 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