{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T17:27:45Z","timestamp":1788542865975,"version":"build-2803163510"},"reference-count":60,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:00:00Z","timestamp":1747180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>This study aimed to assess the protective effects of <jats:italic>Lagenaria siceraria<\/jats:italic> seed oil (LSO) on fifty male Albino rats subjected to furan exposure. Furan (FU) is a small, heterocyclic compound present in the volatile fraction of various thermally processed foods and beverages. Rats were categorized into five groups, each comprising ten rats. Group 1 served as the control group, receiving corn oil. Group 2 received LSO (3\u2009g\/kg body weight orally) for 28 days. Rats in Group 3 (FU-exposed group) received an oral administration of FU at a dosage of 16\u2009mg\/kg body weight each day for 28 days. Rats in Group 4 (Therapeutic co-treated group) were administered both LSO and subsequent FU exposure according to the previously outlined dosage regimen for 28 days. Rats in Group 5 (Protective co-treated group) received LSO seed oil for 14 days as protection then received Fu at the same mentioned doses of Fu until the end of experiment. Rats administered FU and\/or LSO gained noticeably more weight than the control group. LSO significantly decreased AST and LDH levels in both the protection and treatment groups as compared to the FU-only group. It also assisted in restoring testosterone and luteinizing hormone (LH) levels that were decreased by FU, especially in the protected group. LSO also reduced kidney damage markers and normalized biomarker levels when administered with FU. The LSO-only group demonstrated normal immune response markers, similar to the control group. By changing MDA levels and increasing SOD, GSH, and TAC levels, co-treatment with LSO enhanced liver health. The control and LSO groups displayed normal spleen structure, whereas the LSO\/FU group had normal seminiferous tubules with mild edema and congestion. Overall, LSO demonstrated protective and therapeutic benefits against FU-induced damage in rats.<\/jats:p>","DOI":"10.1371\/journal.pone.0322363","type":"journal-article","created":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T17:29:12Z","timestamp":1747243752000},"page":"e0322363","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Protective role of Lagenaria Siceraria seed oil against furan-induced toxicity: Histopathological, biochemical, and molecular insights in male Albino rats"],"prefix":"10.1371","volume":"20","author":[{"given":"Mona","family":"Zahran","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatima S.","family":"Alaryani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khlood M.","family":"El Bohi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ehsan H.","family":"Abu Zeid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed H.","family":"Khairy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aishah E.","family":"Albalawi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reem H.","family":"Alhasani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shatha G.","family":"Felemban","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reda","family":"Korany","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,5,14]]},"reference":[{"key":"pone.0322363.ref001","first-page":"1","article-title":"Comprehensive review for aflatoxin detoxification with special attention to cold plasma treatment","author":"YAG Mahmoud","year":"2025","journal-title":"Mycotoxin Res"},{"key":"pone.0322363.ref002","doi-asserted-by":"crossref","first-page":"160494","DOI":"10.1016\/j.cej.2025.160494","article-title":"Effect of Maillard reaction products on anaerobic digestion of waste activated sludge: Mechanisms from molecular docking and metagenomics","volume":"507","author":"Y Chen","year":"2025","journal-title":"Chem Eng J"},{"issue":"14","key":"pone.0322363.ref003","doi-asserted-by":"crossref","first-page":"2244","DOI":"10.3390\/foods13142244","article-title":"Recent advances in non-contact food decontamination technologies for removing mycotoxins and fungal contaminants","volume":"13","author":"Y Wang","year":"2024","journal-title":"Foods"},{"key":"pone.0322363.ref004","doi-asserted-by":"crossref","first-page":"110944","DOI":"10.1016\/j.ijfoodmicro.2024.110944","article-title":"UV-C light-activated gallic acid and non-thermal technologies for inactivating Salmonella Typhimurium inoculated in aqueous solution and whole cow milk","volume":"427","author":"E Fern\u00e1ndez-Hern\u00e1ndez","year":"2025","journal-title":"Int J Food Microbiol"},{"key":"pone.0322363.ref005","article-title":"Inventory of dioxin and furan emissions to air, land and water in Ireland for 2000 and 2010","author":"F Hayes","year":"2002","journal-title":"EPA Environmental RTDI Programme 2000\u20132006"},{"key":"pone.0322363.ref006","first-page":"53","article-title":"IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. 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Dry Cleaning","volume":"63","author":"IARC (International Agency for Research on Cancer)","year":"1995","journal-title":"Some Chlorinated Solvents and Other Industrial Chemicals"},{"key":"pone.0322363.ref018","first-page":"1","article-title":"Toxicology and carcinogenesis studies of furan (CAS No. 110-00-9) in F344 rats and B6C3F1 mice","volume":"402","author":"National Toxicology Program","year":"1993","journal-title":"Natl Toxicol Program Tech Rep Ser"},{"key":"pone.0322363.ref019","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.fct.2016.06.027","article-title":"Irreversible down-regulation of miR-375 in the livers of Fischer 344 rats after chronic furan exposure","volume":"98","author":"A de Conti","year":"2016","journal-title":"Food Chem Toxicol"},{"issue":"12","key":"pone.0322363.ref020","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1080\/13543776.2018.1552260","article-title":"A decade of nutraceutical patents: where are we now in 2018?","volume":"28","author":"P Daliu","year":"2018","journal-title":"Expert Opin Ther Pat"},{"issue":"9","key":"pone.0322363.ref021","doi-asserted-by":"crossref","first-page":"2221","DOI":"10.1002\/ptr.6419","article-title":"Polyphenols: A concise overview on the chemistry, occurrence, and human health","volume":"33","author":"A Durazzo","year":"2019","journal-title":"Phytother Res"},{"issue":"2","key":"pone.0322363.ref022","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.ijvsm.2013.10.001","article-title":"Antidotal impact of extra virgin olive oil against genotoxicity, cytotoxicity and immunotoxicity induced by hexavalent chromium in rat","volume":"1","author":"S Khalil","year":"2013","journal-title":"Int J Vet Sci Med"},{"issue":"2","key":"pone.0322363.ref023","doi-asserted-by":"crossref","first-page":"67","DOI":"10.36348\/sjbr.2022.v07i02.001","article-title":"Medicinal and nutritional importance of Lagenaria siceraria (Lauki)","volume":"7","author":"M Mehboob","year":"2022","journal-title":"Saudi J Biomed Res"},{"issue":"4","key":"pone.0322363.ref024","first-page":"571","article-title":"Phytochemical screening and pharmacological activity of seed extract and seed oil of Lagenaria siceraria","volume":"2","author":"P Nigam","year":"2016","journal-title":"J Basic Appl Res Biomed"},{"issue":"3","key":"pone.0322363.ref025","first-page":"91","article-title":"Lagenaria siceraria: Phytochemistry, pharmacognosy and pharmacological studies","volume":"2","author":"M Kubde","year":"2010","journal-title":"Report and Opinion"},{"key":"pone.0322363.ref026","unstructured":"El-Dengawy RA, Khalifa AH, Ramadan BR. Use of bottle gourd (Lagenaria siceraria) seeds in production of tahina. 2001."},{"issue":"5","key":"pone.0322363.ref027","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1080\/01480545.2019.1617302","article-title":"Acute and 28-day oral toxicity studies of methanolic extract of Lagenaria siceraria (Cucurbitaceae) fruit in rats","volume":"44","author":"PN Shendge","year":"2021","journal-title":"Drug Chem Toxicol"},{"issue":"8","key":"pone.0322363.ref028","doi-asserted-by":"crossref","first-page":"2016","DOI":"10.5455\/OVJ.2024.v14.i8.31","article-title":"Hepatoprotective efficacy of Lagenaria siceraria seeds oil against experimentally carbon tetrachloride-induced toxicity","volume":"14","author":"NH Almohmadi","year":"2024","journal-title":"Open Vet J"},{"key":"pone.0322363.ref029","volume-title":"Applied Animal Reproduction","author":"HJ Bearden","year":"1980"},{"key":"pone.0322363.ref030","unstructured":"Evans G. Handling and examination of semen. Salamon\u2019s Artificial Insemination of Sheep and Goats. 1987:93\u2013106."},{"key":"pone.0322363.ref031","first-page":"95","article-title":"Serum antiproteases in fish","author":"AE Ellis","year":"1990","journal-title":"Techniques in Fish Immunology"},{"issue":"4","key":"pone.0322363.ref032","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"key":"pone.0322363.ref033","article-title":"Bancroft\u2019s Theory and Practice of Histological Techniques","author":"K Suvarna","year":"2018"},{"key":"pone.0322363.ref034","doi-asserted-by":"crossref","first-page":"1543","DOI":"10.1007\/s10753-023-01826-1","article-title":"Phillygenin ameliorates carbon tetrachloride-induced liver fibrosis: Suppression of inflammation and Wnt\/\u03b2-catenin signaling pathway","volume":"46","author":"C Wang","year":"2023","journal-title":"Inflammation"},{"key":"pone.0322363.ref035","first-page":"1507","article-title":"Evaluation of acute and subchronic toxicity of Lagenaria siceraria aerial parts","volume":"2","author":"P Saha","year":"2011","journal-title":"Int J Pharm Sci Res"},{"key":"pone.0322363.ref036","first-page":"130","article-title":"Hepatoprotective effects of L. siceraria","volume":"2","author":"B Lakshmi","year":"2011","journal-title":"Res J Pharm Biol Chem Sci"},{"key":"pone.0322363.ref037","unstructured":"Tanwar MB, Ninave PB, Navghare VV, Takawale AR, Zope VS, G MM, et al. Hepatoprotective activity of aqueous and ethanolic extract of Trichosanthes dioica Roxb in ferrous sulphate-induced liver injury. 2008."},{"key":"pone.0322363.ref038","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1002\/tox.20673","article-title":"Toxicity of food contaminant furan on liver and kidney of growing male rats","volume":"27","author":"G Selmanoglu","year":"2011","journal-title":"Environ Toxicol"},{"key":"pone.0322363.ref039","doi-asserted-by":"crossref","first-page":"3780","DOI":"10.1007\/s11356-016-8108-9","article-title":"Protective role of quercetin against hematotoxic and immunotoxic effects of furan in rats","volume":"24","author":"RT Alam","year":"2017","journal-title":"Environ Sci Pollut Res"},{"key":"pone.0322363.ref040","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.mrfmmm.2003.12.021","article-title":"Integration of clinical and gene expression endpoints to explore furan-mediated hepatotoxicity","volume":"549","author":"HK Hamadeh","year":"2004","journal-title":"Mutation Research\/Fundamental and Molecular Mechanisms of Mutagenesis"},{"key":"pone.0322363.ref041","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.chemosphere.2019.05.063","article-title":"Toxicological effects of furan on the reproductive system of male rats: An \u201cin vitro\u201d and \u201cin vivo\u201d-based endocrinological and spermatogonial study","volume":"230","author":"H Rehman","year":"2019","journal-title":"Chemosphere"},{"key":"pone.0322363.ref042","first-page":"101","article-title":"Chrysin reduced acrylamide-induced neurotoxicity in both in vitro and in vivo assessments.","volume":"18","author":"S Mehri","year":"2014","journal-title":"Iran Biomed J"},{"key":"pone.0322363.ref043","first-page":"794","article-title":"Free radicals and antioxidants in human health: current status and future prospects","volume":"52","author":"T Devasagayam","year":"2004","journal-title":"Journal of the Association of Physicians of India"},{"key":"pone.0322363.ref044","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1016\/j.bbagen.2009.06.003","article-title":"Is the oxidative stress theory of aging dead?","volume":"1790","author":"VI P\u00e9rez","year":"2009","journal-title":"Biochim Biophys Acta"},{"key":"pone.0322363.ref045","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0891-5849(99)00038-6","article-title":"The effect of exercise training on oxidative damage of lipids, proteins, and DNA in rat skeletal muscle: evidence for beneficial outcomes","volume":"27","author":"Z Rad\u00e1k","year":"1999","journal-title":"Free Radic Biol Med"},{"key":"pone.0322363.ref046","first-page":"1367","article-title":"Hazardous effects of acrylamide on immature male and female rats","volume":"6","author":"SM Rawi","year":"2012","journal-title":"Afr J Pharmacy Pharmacol"},{"key":"pone.0322363.ref047","first-page":"89","article-title":"Metabolic syndrome and male fertility","volume":"36","author":"AD Martins","year":"2018","journal-title":"World J Mens Health"},{"key":"pone.0322363.ref048","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.etap.2014.11.009","article-title":"Toxicological effects of short-term dietary acrylamide exposure in male F344 rats","volume":"39","author":"J Raju","year":"2015","journal-title":"Environ Toxicol Pharmacol"},{"key":"pone.0322363.ref049","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.phrs.2003.10.013","article-title":"Effect of \u03b1-tocopherol and simvastatin on male fertility in hypercholesterolemic rats","volume":"50","author":"M Shalaby","year":"2004","journal-title":"Pharmacol Res"},{"key":"pone.0322363.ref050","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.pestbp.2006.06.010","article-title":"Acute, subacute and subchronic administration of methyl parathion-induced testicular damage in male rats and protective role of vitamins C and E","volume":"87","author":"M Uzunhisarcikli","year":"2007","journal-title":"Pest Biochem Physiol"},{"key":"pone.0322363.ref051","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1089\/ars.2010.3186","article-title":"Redox regulation of human sperm function: from the physiological control of sperm capacitation to the etiology of infertility and DNA damage in the germ line","volume":"14","author":"RJ Aitken","year":"2011","journal-title":"Antioxid Redox Signal"},{"key":"pone.0322363.ref052","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1016\/j.fct.2010.02.026","article-title":"Effects of heat-induced food contaminant furan on reproductive system of male rats from weaning through postpuberty","volume":"48","author":"E Karacaoglu","year":"2010","journal-title":"Food Chem Toxicol"},{"key":"pone.0322363.ref053","doi-asserted-by":"crossref","first-page":"1815","DOI":"10.1016\/S0278-6915(02)00168-0","article-title":"Lack of effects of oxolinic acid on spermatogenesis in young adult and aged Wistar rats","volume":"40","author":"M Yoshida","year":"2002","journal-title":"Food Chem Toxicol"},{"key":"pone.0322363.ref054","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.taap.2012.11.025","article-title":"Protection of cisplatin-induced spermatotoxicity, DNA damage and chromatin abnormality by selenium nano-particles","volume":"266","author":"MA Rezvanfar","year":"2013","journal-title":"Toxicol Appl Pharmacol"},{"issue":"8","key":"pone.0322363.ref055","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.molmed.2009.06.005","article-title":"p38MAPK: Stress responses from molecular mechanisms to therapeutics","volume":"15","author":"LR Coulthard","year":"2009","journal-title":"Trends Mol Med"},{"issue":"1","key":"pone.0322363.ref056","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1002\/mc.20571","article-title":"Curcumin-induced apoptosis in ovarian carcinoma cells is p53-independent and involves p38 mitogen-activated protein kinase activation and 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cascades","volume":"410","author":"L Chang","year":"2001","journal-title":"Nature"},{"key":"pone.0322363.ref060","doi-asserted-by":"crossref","DOI":"10.1016\/S0092-8674(02)00703-1","article-title":"Missing pieces in the NF-kappaB puzzle","volume":"109","author":"S Ghosh","year":"2002","journal-title":"Cell"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0322363","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T17:29:26Z","timestamp":1747243766000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0322363"}},"subtitle":[],"editor":[{"given":"Abeer","family":"El 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