{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T22:57:03Z","timestamp":1792018623159,"version":"4.4.0"},"reference-count":76,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T00:00:00Z","timestamp":1748476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Due to the need to produce high-quality and healthy eggs, the current experiment was conducted to investigate the impact of dietary supplementation of whey powder (WP), <jats:italic>Bacillus coagulans (B. coagulans<\/jats:italic>), and their combination (MIX) on the production performance, egg quality, blood biochemical parameters, and histomorphological parameters of Lohmann LSL-lite laying hens. 144 Lohmann laying hen (75 weeks) were randomly assigned to 4 different dietary treatments, with 6 replications and 6 hens per cage. The hens were fed a basal diet (control, CON), the basal diet supplemented with 1\u2009g\/kg WP, 1\u2009g\/kg <jats:italic>B. coagulans<\/jats:italic> (4\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> CFU), and 1\u2009g\/kg WP plus 1\u2009g\/kg <jats:italic>B. coagulans<\/jats:italic> probiotic for 12 weeks. Feed intake and egg weight were not affected by the treatments at any stage of the trial (<jats:italic>P<\/jats:italic>\u2009&gt;\u20090.05). No significant interaction was found between WP and <jats:italic>B. coagulans<\/jats:italic> in egg quality parameters and blood-biochemical parameters other than malondialdehyde (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). The level of malondialdehyde in serum was reduced when WP was used along with <jats:italic>B. coagulans<\/jats:italic> compared to when WP was used alone. However, egg production in all periods and egg mass in the first period were affected by the synergistic effect of WP and <jats:italic>B. coagulans<\/jats:italic>. Furthermore, FCR was reduced in the first period (75\u201380 weeks) under the influence of the MIX group compared to the control group or when used alone (P\u2009&lt;\u20090.05). The color of the yolk was increased in the group receiving <jats:italic>B. coagulans<\/jats:italic> compared to the control group (P\u2009&lt;\u20090.05). Therefore, in birds fed with <jats:italic>B. coagulans<\/jats:italic>, a significant increase in the width of the villi in the ileum was observed (P\u2009\u2264\u20090.05). Interestingly, <jats:italic>B. coagulans<\/jats:italic> and WP reduced performance when used alone compared to the control, but improved performance when combined. In conclusion, the simultaneous use of WP and <jats:italic>B. coagulans<\/jats:italic> in diet can probably improve the parameters of production performance, FCR, and serum malondialdehyde level at the end of the production period of Lohmann laying hens.<\/jats:p>","DOI":"10.1371\/journal.pone.0322557","type":"journal-article","created":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T20:04:23Z","timestamp":1748549063000},"page":"e0322557","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effects of dietary of Bacillus coagulans, whey powder, and their interaction on the performance of Lohmann LSL-lite laying hens in the late production phase"],"prefix":"10.1371","volume":"20","author":[{"given":"Zahra","family":"Hamzehee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5660-0273","authenticated-orcid":true,"given":"Mehran","family":"Torki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khodabakhsh","family":"Rashidi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alireza","family":"Abdolmohammadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,5,29]]},"reference":[{"key":"pone.0322557.ref001","volume-title":"Probiotics: alternative to antibiotics in poultry production","author":"S Rashid","year":"2023"},{"issue":"8","key":"pone.0322557.ref002","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.3390\/antibiotics12081303","article-title":"Regulations on the use of antibiotics in livestock production in South America: a comparative literature analysis","volume":"12","author":"RA Da Silva","year":"2023","journal-title":"Antibiotics (Basel)"},{"key":"pone.0322557.ref003","volume-title":"OECD-FAO Agricultural Outlook, 2020\u20132029. 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