{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T06:39:17Z","timestamp":1773383957057,"version":"3.50.1"},"reference-count":52,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T00:00:00Z","timestamp":1746403200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016308","name":"Bpifrance","doi-asserted-by":"publisher","award":["PSPC-402492"],"award-info":[{"award-number":["PSPC-402492"]}],"id":[{"id":"10.13039\/100016308","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Autotransfusion is a key strategy in hemorrhagic surgical procedures, reducing risks like disease transmission and immunosuppression due to allogenic transfusion. While conventional devices efficiently process red blood cells by centrifugation, they don\u2019t address complications requiring additional platelet transfusions. The innovative same\u2122 device (i-SEP, France), utilizing hollow-fiber filtration, preserves both red blood cells and platelets without damaging cell integrity. This study designed as a prospective randomized controlled trial compared cell concentration and washout performances of two autotransfusion devices, a preclinical version of same\u2122, the conventional centrifugation-based Xtra\u00ae (LivaNova, UK); and effects of retransfusion versus no transfusion in 21 Yucatan minipigs submitted to a surgically induced controlled splenic bleeding. Animals were divided into three groups (no-transfusion <jats:italic>control<\/jats:italic> group, <jats:italic>same<\/jats:italic> group and <jats:italic>xtra<\/jats:italic> group) and monitored postoperatively for 72 hours during which serial animal blood samples were collected for hematologic, biochemical and coagulation analyses and tests. Both autotransfusion devices showed high performances in red blood cell yields and concentrations, with a significant superiority of same\u2122 device to preserve platelets. Animals from <jats:italic>same<\/jats:italic> and <jats:italic>xtra<\/jats:italic> groups retrieved similar rheological profiles and maintained a normal hematocrit compared to no-transfusion control animals. Coagulation profiles stayed within normal range in all groups. No adverse event on animals nor post-mortem sign of thrombosis were identified on autotransfused animals. The same\u2122 device that can therefore be considered as an interesting alternative to conventional centrifugation-based devices. Further experiments are needed to provide evidence of platelets autotransfusion benefits in massive hemorrhagic procedures.<\/jats:p>","DOI":"10.1371\/journal.pone.0322568","type":"journal-article","created":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T17:28:07Z","timestamp":1746466087000},"page":"e0322568","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparative evaluation of two autotransfusion devices in a 72h survival swine model of surgically induced controlled splenic bleeding"],"prefix":"10.1371","volume":"20","author":[{"given":"Axelle","family":"Castelli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chlo\u00e9","family":"Libaud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beno\u00eet","family":"Decouture","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marine","family":"Bruneau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mallorie","family":"Depond","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Forest-Villegas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Gauthier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Audrey","family":"Lafragette","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9176-0715","authenticated-orcid":true,"given":"Gwenola","family":"Touzot-Jourde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,5,5]]},"reference":[{"key":"pone.0322568.ref001","doi-asserted-by":"crossref","DOI":"10.1002\/14651858.CD001888.pub3","article-title":"Cell salvage for minimising perioperative allogeneic blood transfusion.","volume-title":"Cochrane Database of Systematic Reviews","author":"PA Carless","year":"2010"},{"key":"pone.0322568.ref002","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1186\/s12913-018-3673-x","article-title":"Improvements in blood transfusion management: cross-sectional data analysis from nine hospitals in Zhejiang, China","volume":"18","author":"Y Yao","year":"2018","journal-title":"BMC Health Serv Res"},{"issue":"4","key":"pone.0322568.ref003","doi-asserted-by":"crossref","first-page":"152","DOI":"10.33545\/orthor.2018.v2.i4c.88","article-title":"Effect of intra-operative cell salvage in negating the need for allogenic transfusion in patients undergoing primary hip and knee arthroplasty","volume":"2","author":"DrD Sharma","year":"2018","journal-title":"National Journal of Clinical Orthopaedics"},{"key":"pone.0322568.ref004","doi-asserted-by":"crossref","first-page":"15","DOI":"10.4103\/0971-9784.38444","article-title":"Blood transfusion is associated with increased resource utilisation, morbidity and mortality in cardiac surgery","volume":"11","author":"B Scott","year":"2008","journal-title":"Ann Card Anaesth"},{"key":"pone.0322568.ref005","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1111\/j.1423-0410.2012.01584.x","article-title":"Current incidence and residual risk of HIV, HBV and HCV at Canadian Blood Services","volume":"103","author":"SF O\u2019Brien","year":"2012","journal-title":"Vox Sang"},{"key":"pone.0322568.ref006","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.transci.2013.12.010","article-title":"The ONTraC Ontario program in blood conservation","volume":"50","author":"J Freedman","year":"2014","journal-title":"Transfusion and Apheresis Science"},{"key":"pone.0322568.ref007","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1001\/jama.2014.2726","article-title":"Health care-associated infection after red blood cell transfusion","volume":"311","author":"JM Rohde","year":"2014","journal-title":"JAMA"},{"key":"pone.0322568.ref008","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1093\/bjaceaccp\/mkl042","article-title":"Autologous blood transfusion","volume":"6","author":"A Walunj","year":"2006","journal-title":"Continuing Education in Anaesthesia Critical Care & Pain"},{"key":"pone.0322568.ref009","first-page":"1501","article-title":"Cost of allogeneic and autologous blood transfusion in Canada. 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