{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T11:00:27Z","timestamp":1775473227425,"version":"3.50.1"},"reference-count":99,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T00:00:00Z","timestamp":1747699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Roadkill is a global issue contributing to biodiversity decline which is increasingly recognized by scientists and decision-makers. In Cyprus, a biodiversity hotspot with one of the highest road densities in Europe, the impact of roads on wildlife has been largely overlooked due to the absence of life-threatening collisions. We analysed data from a 10-year roadkill dataset (2013\u20132022) collected systematically by the Department of Public Works across 27 main roads, primarily aimed at driver safety. Due to the absence of spatial attributes for roadkill locations, the dataset was analysed to identify taxonomic, seasonal, and temporal roadkill trends in Cyprus for the first time. A total of 1,985 roadkill incidents were recorded, involving seven wildlife taxa: foxes (44%), birds (26%), hedgehogs (11.5%), snakes (7%), hares, rats, and lizards. Most roadkill occurred in the Nicosia district (65%). Statistical analyses using chi-square tests with post-hoc Bonferroni corrections revealed strong associations between road types and taxa. Fox and bird roadkill incidents were most common on highways, while hedgehogs and snakes were frequent on secondary interurban or rural roads. Roads near protected areas exhibited higher roadkill frequencies than highways. A positive relationship between traffic volume and roadkill risk was evident, with higher traffic roads posing greater risks. Seasonal patterns showed increased roadkill during spring and summer, peaking in June. This synthesis provides crucial insights into roadkill patterns, offering guidance for conservation and mitigation actions. However, the current monitoring system, focused on driver safety, is inadequate for comprehensive roadkill reporting. Key limitations, such as the absence of spatial data and weaknesses in the protocol, have been identified, with recommendations for improvement proposed to enhance future monitoring efforts.<\/jats:p>","DOI":"10.1371\/journal.pone.0322644","type":"journal-article","created":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T20:01:36Z","timestamp":1747771296000},"page":"e0322644","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Roadkill in a Mediterranean island: Evaluating ten-years of official records"],"prefix":"10.1371","volume":"20","author":[{"given":"Ioannis 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opportunities","volume":"42","author":"C Soulsbury","year":"2015","journal-title":"Wildl Res"},{"key":"pone.0322644.ref002","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.biocon.2017.11.019","article-title":"Habitat use, ranching, and human-wildlife conflict within a fragmented landscape in the Pantanal, Brazil","volume":"217","author":"JC de Souza","year":"2018","journal-title":"Biol Conserv"},{"key":"pone.0322644.ref003","article-title":"Levels of conflict over wildlife: Understanding and addressing the right problem","volume":"2","author":"A Zimmermann","year":"2020","journal-title":"Conserv Sci Pract"},{"key":"pone.0322644.ref004","doi-asserted-by":"crossref","DOI":"10.1038\/s41558-023-01608-5","article-title":"Climate change as a global amplifier of human\u2013wildlife conflict","volume":"13","author":"B Abrahms","year":"2023","journal-title":"Nat Clim 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D\u2019Amico","year":"2018","journal-title":"Eur J Wildl Res"},{"key":"pone.0322644.ref016","doi-asserted-by":"crossref","DOI":"10.1111\/geb.12564","article-title":"Global exposure of carnivores to roads: exposure of carnivores to roads","volume":"26","author":"A Ceia-Hasse","year":"2017","journal-title":"Glob Ecol Biogeogr"},{"key":"pone.0322644.ref017","article-title":"Wildlife mortality risk posed by high and low traffic roads","volume":"38","author":"D Denneboom","year":"2023","journal-title":"Conserv Biol"},{"issue":"7797","key":"pone.0322644.ref018","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1038\/s41586-020-2022-5","article-title":"A simple dynamic model explains the diversity of island birds worldwide","volume":"579","author":"L Valente","year":"2020","journal-title":"Nature"},{"issue":"19","key":"pone.0322644.ref019","doi-asserted-by":"crossref","DOI":"10.1016\/j.cub.2021.07.033","article-title":"Island biogeography","volume":"31","author":"TJ 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Ascens\u00e3o","year":"2021","journal-title":"Biol Conserv"},{"key":"pone.0322644.ref027","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.biocon.2019.07.014","article-title":"The magnitude of roadkill in Taiwan: patterns and consequences revealed by citizen science","volume":"237","author":"K Chyn","year":"2019","journal-title":"Biol Conserv"},{"key":"pone.0322644.ref028","doi-asserted-by":"crossref","first-page":"125798","DOI":"10.1016\/j.jnc.2020.125798","article-title":"Benefits and challenges of collaborating with volunteers: examples from national wildlife roadkill reporting systems in Europe","author":"M B\u00edl","year":"2020","journal-title":"J Nat Conserv"},{"key":"pone.0322644.ref029","doi-asserted-by":"crossref","first-page":"89","DOI":"10.3389\/fevo.2017.00089","article-title":"Large extent volunteer roadkill and wildlife observation systems as sources of reliable data","volume":"5","author":"D Waetjen","year":"2017","journal-title":"Front Ecol 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BOU Checklist n. o6","author":"P Flint","year":"1992","journal-title":"Br Ornithol Union Tring"},{"key":"pone.0322644.ref042","first-page":"695","article-title":"Aves.","volume-title":"An introduction to the wildlife of Cyprus","author":"I Charalambidou","year":"2016"},{"key":"pone.0322644.ref043","volume-title":"Road transport: Length of public roads and total expenditure on roads and bridges, 1985-2022","author":"Statistical Service","year":"2022"},{"key":"pone.0322644.ref044","volume-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team","year":"2023"},{"key":"pone.0322644.ref045","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.biocon.2012.09.006","article-title":"Vertebrate road mortality estimates: Effects of sampling methods and carcass removal","volume":"157","author":"FZ Teixeira","year":"2013","journal-title":"Biol Conserv"},{"issue":"9","key":"pone.0322644.ref046","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0025383","article-title":"How long do the dead survive on the road? Carcass persistence probability and implications for road-kill monitoring surveys","volume":"6","author":"SM Santos","year":"2011","journal-title":"PLoS One"},{"issue":"1","key":"pone.0322644.ref047","article-title":"Roadkill scavenging behaviour in an urban environment","volume":"4","author":"ALW Schwartz","year":"2018","journal-title":"J Urban Ecol"},{"key":"pone.0322644.ref048","article-title":"Carcass permanency time and its implications to the roadkill data","volume":"60","author":"P Ratton","year":"2014","journal-title":"Eur J Wildl Res"},{"key":"pone.0322644.ref049","doi-asserted-by":"crossref","first-page":"121","DOI":"10.3897\/natureconservation.47.72970","article-title":"Animals under wheels: Wildlife roadkill data collection by citizen scientists as a part of their nature recording activities","volume":"47","author":"K Swinnen","year":"2022","journal-title":"Nat Conserv"},{"key":"pone.0322644.ref050","unstructured":"Vogiatzakis IN, Zotos S, Litskas V, Leontiou S. Assessment of the impacts of the road network on the wildlife of Cyprus. 2022."},{"key":"pone.0322644.ref051","doi-asserted-by":"crossref","first-page":"109012","DOI":"10.1016\/j.biocon.2021.109012","article-title":"COVID-19 restrictions provide a brief respite from the wildlife roadkill toll","volume":"256","author":"MM Driessen","year":"2021","journal-title":"Biol Conserv"},{"issue":"6","key":"pone.0322644.ref052","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1111\/1365-2656.13913","article-title":"The impact of the COVID-19 lockdowns on wildlife-vehicle collisions in the UK","volume":"92","author":"S Raymond","year":"2023","journal-title":"J Anim Ecol"},{"key":"pone.0322644.ref053","doi-asserted-by":"crossref","DOI":"10.1111\/1365-2664.14140","article-title":"Wildlife roadkill and COVID-19: a biologically significant, but heterogeneous, reduction","volume":"59","author":"B Pokorny","year":"2022","journal-title":"J Appl 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Santos","year":"2016","journal-title":"Biol Conserv"},{"issue":"1","key":"pone.0322644.ref058","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1111\/j.1365-2664.2011.02068.x","article-title":"The effect of a major road on bat activity and diversity","volume":"49","author":"A Berthinussen","year":"2011","journal-title":"J Appl Ecol"},{"issue":"2","key":"pone.0322644.ref059","article-title":"Estimates of avian mortality attributed to vehicle collisions in Canada","volume":"8","author":"CA Bishop","year":"2013","journal-title":"ACE"},{"key":"pone.0322644.ref060","article-title":"Carnivore abundance near motorways related to prey and roadkills","volume":"82","author":"A Planillo","year":"2017","journal-title":"J Wildl Manage"},{"issue":"1","key":"pone.0322644.ref061","doi-asserted-by":"crossref","DOI":"10.1093\/jue\/juaa039","article-title":"Life in the fast lane: roadkill risk along an urban\u2013rural gradient","volume":"7","author":"E Kent","year":"2021","journal-title":"J Urban Ecol"},{"issue":"3","key":"pone.0322644.ref062","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0118604","article-title":"Transport infrastructure shapes foraging habitat in a raptor community","volume":"10","author":"A Planillo","year":"2015","journal-title":"PLoS One"},{"issue":"1","key":"pone.0322644.ref063","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1111\/brv.12093","article-title":"Animal reactions to oncoming vehicles: a conceptual review","volume":"90","author":"SL Lima","year":"2015","journal-title":"Biol Rev Camb Philos Soc"},{"key":"pone.0322644.ref064","doi-asserted-by":"crossref","DOI":"10.1111\/acv.12120","article-title":"Understanding the mechanisms behind road effects: linking occurrence with road mortality in owls","volume":"17","author":"C Grilo","year":"2014","journal-title":"Anim Conserv"},{"issue":"1","key":"pone.0322644.ref065","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.anbehav.2010.04.007","article-title":"Nocturnal ranging behaviour of urban hedgehogs, Erinaceus europaeus, in relation to risk and reward","volume":"80","author":"CV Dowding","year":"2010","journal-title":"Anim Behav"},{"key":"pone.0322644.ref066","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1046\/j.1365-2435.2002.00651.x","article-title":"Roads as barriers to movement for hedgehogs","volume":"16","author":"C Rondinini","year":"2002","journal-title":"Funct Ecol"},{"key":"pone.0322644.ref067","article-title":"Predicting hedgehog mortality risks on British roads using habitat suitability modelling","volume":"7","author":"PGR Wright","year":"2020","journal-title":"PeerJ"},{"issue":"1","key":"pone.0322644.ref068","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s12898-017-0134-z","article-title":"Amphibian and reptile road-kills on tertiary roads in relation to landscape structure: using a citizen science approach with open-access land cover data","volume":"17","author":"F Heigl","year":"2017","journal-title":"BMC Ecol"},{"key":"pone.0322644.ref069","doi-asserted-by":"crossref","unstructured":"Andrews K, Langen T, Struijk R. Reptiles: overlooked but often at risk from roads. 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Pagany","year":"2020","journal-title":"Biol Conserv"},{"key":"pone.0322644.ref074","doi-asserted-by":"crossref","first-page":"115272","DOI":"10.1016\/j.jenvman.2022.115272","article-title":"Effects of roads on small-mammal movements: Opportunities and risks of vegetation management on roadsides","volume":"316","author":"A Galantinho","year":"2022","journal-title":"J Environ Manage"},{"issue":"4","key":"pone.0322644.ref075","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1016\/j.jenvman.2010.11.027","article-title":"Spatial wildlife-vehicle collision models: a review of current work and its application to transportation mitigation projects","volume":"92","author":"KE Gunson","year":"2011","journal-title":"J Environ Manage"},{"key":"pone.0322644.ref076","article-title":"Effective wildlife roadkill mitigation","volume":"3","author":"D Lester","year":"2015","journal-title":"J Traffic Transp 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Res"},{"issue":"1","key":"pone.0322644.ref080","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1046\/j.1523-1739.2000.99088.x","article-title":"The ecological road-effect zone of a Massachusetts (U.S.A.) suburban highway","volume":"14","author":"R Forman","year":"2000","journal-title":"Conserv Biol"},{"key":"pone.0322644.ref081","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.ecolmodel.2004.12.015","article-title":"Predicting when animal populations are at risk from roads: an interactive model of road avoidance behavior","volume":"185","author":"J Jaeger","year":"2005","journal-title":"Ecol Modell"},{"issue":"2","key":"pone.0322644.ref082","doi-asserted-by":"crossref","DOI":"10.1890\/ES10-00093.1","article-title":"Evaluating the road-effect zone on wildlife distribution in a rural landscape","volume":"2","author":"CS Shanley","year":"2011","journal-title":"Ecosphere"},{"issue":"4","key":"pone.0322644.ref083","doi-asserted-by":"crossref","DOI":"10.1002\/ecs2.1345","article-title":"A behavior\u2010based framework for assessing barrier effects to wildlife from vehicle traffic volume","volume":"7","author":"SL Jacobson","year":"2016","journal-title":"Ecosphere"},{"key":"pone.0322644.ref084","volume-title":"Ecological effects of roads: a review","author":"A Seiler","year":"2001"},{"key":"pone.0322644.ref085","article-title":"Barrier effects of roads on movements of small mammals","volume":"56","author":"A Cernohorska","year":"2007","journal-title":"Folia Zool"},{"issue":"2","key":"pone.0322644.ref086","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1353\/gpr.2017.0025","article-title":"Roads and ecological infrastructure: concepts and applications for small animals","volume":"27","author":"S Vantassel","year":"2017","journal-title":"Great Plains Res"},{"key":"pone.0322644.ref087","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/geb.13557","article-title":"Roadkill patterns in Latin American birds and mammals","volume":"31","author":"P Medrano-Vizca\u00edno","year":"2022","journal-title":"Glob Ecol Biogeogr"},{"key":"pone.0322644.ref088","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10980-023-01671-5","article-title":"How did the animal come to cross the road? Drawing insights on animal movement from existing roadkill data and expert knowledge","volume":"38","author":"I Cassimiro","year":"2023","journal-title":"Landsc Ecol"},{"key":"pone.0322644.ref089","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.landurbplan.2016.05.029","article-title":"Seasonal variation in vertebrate traffic casualties and its implications for mitigation measures","volume":"157","author":"N Garriga","year":"2017","journal-title":"Landsc Urban Plan"},{"issue":"10","key":"pone.0322644.ref090","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0258083","article-title":"Temporal patterns of wildlife roadkill in the UK","volume":"16","author":"S Raymond","year":"2021","journal-title":"PLoS One"},{"key":"pone.0322644.ref091","volume-title":"National action plan for the conservation of species Hierophis cypriensis (Cyprus whip snake)","author":"S Zotos","year":"2022"},{"key":"pone.0322644.ref092","article-title":"Protecting the protected: reducing wildlife roadkill in protected areas","volume":"22","author":"W CollinsonJonker","year":"2019","journal-title":"Anim Conserv"},{"issue":"7","key":"pone.0322644.ref093","doi-asserted-by":"crossref","first-page":"737","DOI":"10.3390\/land10070737","article-title":"Sustainable Landscape Planning to Mitigate Wildlife\u2013Vehicle Collisions","volume":"10","author":"A Fedorca","year":"2021","journal-title":"Land"},{"issue":"11","key":"pone.0322644.ref094","doi-asserted-by":"crossref","first-page":"2761","DOI":"10.1007\/s10531-012-0332-0","article-title":"Are protected areas truly protected? The impact of road traffic on vertebrate fauna","volume":"21","author":"N Garriga","year":"2012","journal-title":"Biodivers Conserv"},{"issue":"9","key":"pone.0322644.ref095","doi-asserted-by":"crossref","first-page":"2343","DOI":"10.1007\/s10980-022-01491-z","article-title":"Stay home, stay safe? High habitat suitability and environmental connectivity increases road mortality in a colonizing mesocarnivore","volume":"37","author":"L Frangini","year":"2022","journal-title":"Landsc Ecol"},{"issue":"6","key":"pone.0322644.ref096","doi-asserted-by":"crossref","DOI":"10.1007\/s10344-023-01750-7","article-title":"Examining vertebrate road mortality on highways passing through protected areas of eastern Ethiopia","volume":"69","author":"G Mulualem","year":"2023","journal-title":"Eur J Wildl Res"},{"key":"pone.0322644.ref097","doi-asserted-by":"crossref","unstructured":"Zotos S, Baier F, Sparrow D, Vogiatzakis IN. A citizen science approach to assess the impact of roads on reptile mortality in Cyprus. In: Sixth International Conference on Remote Sensing and Geoinformation of the Environment (RSCy2018). SPIE. 2018. 168\u201380.","DOI":"10.1117\/12.2326222"},{"issue":"30","key":"pone.0322644.ref098","first-page":"675","article-title":"Nonfatal motor-vehicle animal crash-related injuries--United States, 2001-2002","volume":"53","author":"Centers for Disease Control and Prevention","year":"2004","journal-title":"MMWR Morb Mortal Wkly Rep"},{"issue":"6","key":"pone.0322644.ref099","doi-asserted-by":"crossref","first-page":"1865","DOI":"10.1016\/j.aap.2008.08.002","article-title":"Road crashes involving animals in Australia","volume":"40","author":"P Rowden","year":"2008","journal-title":"Accid Anal Prev"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0322644","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T20:02:09Z","timestamp":1747771329000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0322644"}},"subtitle":[],"editor":[{"given":"Juan","family":"Scheun","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2025,5,20]]},"references-count":99,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2025,5,20]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0322644","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,20]]}}}