{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T15:21:29Z","timestamp":1783524089745,"version":"3.55.0"},"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T00:00:00Z","timestamp":1747958400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Mosquitoes are important vectors for various infectious pathogens. More than 200 species of mosquitoes are common in Vietnam, one of the main carriers of viruses that are important for humans is the mosquito of the genus <jats:italic>Aedes aegypti<\/jats:italic>. Metavirome sequencing can shed light on the diversity of mosquito-borne viruses classified as insect-specific viruses (ISV). After BLAST analysis using the viral database, contigs were classified as belonging to seventeen ISVs. Ten of them are distributed among five families: <jats:italic>Totiviridae<\/jats:italic>; <jats:italic>Flaviviridae<\/jats:italic>; <jats:italic>Partitiviridae<\/jats:italic>; <jats:italic>Phenuiviridae;<\/jats:italic> and <jats:italic>Orthomyxoviridae<\/jats:italic>. The other seven species belonged to recently identified RNA viruses whose taxonomic position is undefined in the current classification of the International Committee on Taxonomy of Viruses (ICTV). This is the first study to reveal the diversity of RNA viruses associated with <jats:italic>Aedes aegypti<\/jats:italic> mosquitoes in Vietnam, while highlighting the need for further study of ISV in mosquito vectors.<\/jats:p>","DOI":"10.1371\/journal.pone.0322924","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T20:03:26Z","timestamp":1748030606000},"page":"e0322924","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["High throughput sequencing sheds light on the viral diversity in Aedes aegypti mosquitoes from Binh Thuan Province, Vietnam"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-1268-311X","authenticated-orcid":true,"given":"Margarita","family":"Popova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Gladkikh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2086-7480","authenticated-orcid":true,"given":"Alena","family":"Sharova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"Arbuzova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeriya","family":"Sbarzaglia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina","family":"Klyuchnikova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadezhda","family":"Tsyganova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majid","family":"Forghani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia","family":"Gritseva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward","family":"Ramsay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Thanh","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bui Thanh","family":"Phu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2097-7364","authenticated-orcid":true,"given":"Do Thai","family":"Hung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5500-0169","authenticated-orcid":true,"given":"Vladimir","family":"Dedkov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,5,23]]},"reference":[{"key":"pone.0322924.ref001","author":"World Health Organization","year":"2014"},{"issue":"4","key":"pone.0322924.ref002","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1038\/s41579-021-00639-z","article-title":"Infectious disease in an era of global change","volume":"20","author":"RE Baker","year":"2022","journal-title":"Nat Rev Microbiol"},{"issue":"11","key":"pone.0322924.ref003","doi-asserted-by":"crossref","first-page":"2041","DOI":"10.1007\/s10096-017-3030-8","article-title":"Zika virus infection in Vietnam: current epidemic, strain origin, spreading risk, and perspective","volume":"36","author":"D-T Chu","year":"2017","journal-title":"Eur J Clin Microbiol Infect Dis"},{"issue":"11","key":"pone.0322924.ref004","first-page":"24","article-title":"Demographic and clinical profile of dengue fever in a tertiary care hospital of South India","volume":"68","author":"KV Padmaprakash","year":"2020","journal-title":"J Assoc Physicians India"},{"key":"pone.0322924.ref005","year":"2024"},{"issue":"1","key":"pone.0322924.ref006","first-page":"1660129","article-title":"Urban transmission of mosquito-borne flaviviruses - 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