{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T04:44:02Z","timestamp":1790484242734,"version":"4.1.0"},"reference-count":65,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"IST Carver Mead Endowment","award":["25550038"],"award-info":[{"award-number":["25550038"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>The ability to image blood flow in early-stage avian embryos has significant applications in developmental biology, drug and vaccine testing, as well as determining sex differentiation. In this project, a recently developed laser speckle contrast imaging (LSCI) system was used to non-invasively image extraembryonic blood vessels and used these images to attempt early sex identification of chick embryos. Specifically, blood vessels images were captured from 1,251 living chicken embryos between day three and day four of incubation. Then, deep neural network (DNN) models were applied to evaluate whether it is possible to differentiate sex based on vascular patterns. Using ResNetBiT and YOLOv5s-cls models, our results indicate that sex differentiation from extraembryonic blood vessel images was not achievable with sufficiently high accuracy or statistical significance for practical use. Specifically, ResNetBiT had a five-fold cross-validated average accuracy of 56%\u2009\u00b1\u20094% (p-value of 0.28 across cross-validation folds) at day 3 and 57%\u2009\u00b1\u20093% (p-value of 0.07 across cross-validation folds) at day 4. YOLOv5s-cls had a five-fold cross-validated average accuracy of 55%\u2009\u00b1\u20092% (p-value of 0.13 across folds) at day 3 and 57%\u2009\u00b1\u20093% (p-value of 0.10 across folds) at day 4. Our findings suggest that under the current experimental conditions and modeling approaches, per-egg evaluation did not produce sufficiently accurate or statistically robust results for early sex classification.<\/jats:p>","DOI":"10.1371\/journal.pone.0323847","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T17:24:36Z","timestamp":1782494676000},"page":"e0323847","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Detection of non-invasive sexing of early chick embryos in intact eggs using laser speckle contrast imaging and deep neural 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imaging","volume":"31","author":"P Li","year":"2006","journal-title":"Opt Lett"},{"key":"pone.0323847.ref037","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1109\/RBME.2013.2243140","article-title":"Laser speckle contrast imaging: Theory, instrumentation and applications","volume":"6","author":"J Senarathna","year":"2013","journal-title":"IEEE Rev Biomed Eng"},{"issue":"18","key":"pone.0323847.ref038","doi-asserted-by":"crossref","first-page":"28461","DOI":"10.1364\/OE.431989","article-title":"Real-time monitoring of biomechanical activity in aphids by laser speckle contrast imaging","volume":"29","author":"S Yordanov","year":"2021","journal-title":"Opt Express"},{"issue":"6","key":"pone.0323847.ref039","doi-asserted-by":"crossref","first-page":"3571","DOI":"10.1364\/BOE.426521","article-title":"Choosing a model for laser speckle contrast imaging","volume":"12","author":"C Liu","year":"2021","journal-title":"Biomed Opt 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Smith","year":"2004","journal-title":"Bioessays"},{"issue":"1","key":"pone.0323847.ref046","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1007\/s10577-011-9263-y","article-title":"The potential role of microRNAs in regulating gonadal sex differentiation in the chicken embryo","volume":"20","author":"AD Cutting","year":"2012","journal-title":"Chromosome Res"},{"issue":"1","key":"pone.0323847.ref047","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1080\/713655025","article-title":"Sexing chick embryos: A rapid and simple protocol","volume":"42","author":"M Clinton","year":"2001","journal-title":"Br Poult Sci"},{"issue":"1","key":"pone.0323847.ref048","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1002\/jmor.1050880104","article-title":"A series of normal stages in the development of the chick embryo","volume":"88","author":"V Hamburger","year":"1951","journal-title":"J Morphol"},{"key":"pone.0323847.ref049","doi-asserted-by":"crossref","unstructured":"Deng J, Dong W, Socher R, Li L-J, Kai Li, Li FF. ImageNet: A large-scale hierarchical image database. 2009 IEEE Conference on Computer Vision and Pattern Recognition, 2009. 248\u201355. https:\/\/doi.org\/10.1109\/cvpr.2009.5206848","DOI":"10.1109\/CVPR.2009.5206848"},{"issue":"9","key":"pone.0323847.ref050","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1109\/TKDE.2008.239","article-title":"Learning from imbalanced data","volume":"21","author":"He Haibo","year":"2009","journal-title":"IEEE Trans Knowl Data Eng"},{"key":"pone.0323847.ref051","unstructured":"Yosinski J, Clune J, Bengio Y, Lipson H. How transferable are features in deep neural networks?. 2014. https:\/\/doi.org\/10.48550\/ARXIV.1411.1792"},{"issue":"3","key":"pone.0323847.ref052","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1109\/83.826787","article-title":"Image enhancement via adaptive unsharp masking","volume":"9","author":"A Polesel","year":"2000","journal-title":"IEEE Trans Image Process"},{"issue":"5","key":"pone.0323847.ref053","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1109\/TIP.2010.2092441","article-title":"A generalized unsharp masking algorithm","volume":"20","author":"G Deng","year":"2011","journal-title":"IEEE Trans Image Process"},{"key":"pone.0323847.ref054","doi-asserted-by":"crossref","first-page":"3435","DOI":"10.1007\/s13369-023-07986-4","article-title":"SEBR: Scharr edge-based regularization method for blind image deblurring","volume":"49","author":"N Bibi","year":"2024","journal-title":"Arab J Sci Eng"},{"issue":"1","key":"pone.0323847.ref055","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1023\/B:VLSI.0000028532.53893.82","article-title":"Realization of the Contrast Limited Adaptive Histogram Equalization (CLAHE) for real-time image enhancement","volume":"38","author":"AM Reza","year":"2004","journal-title":"The Journal of VLSI Signal Processing-Systems for Signal, Image, and Video Technology"},{"key":"pone.0323847.ref056","doi-asserted-by":"crossref","unstructured":"Musa P, Rafi FA, Lamsani M. A Review: Contrast-Limited Adaptive Histogram Equalization (CLAHE) methods to help the application of face recognition. 2018 Third International Conference on Informatics and Computing (ICIC), 2018. 1\u20136. https:\/\/doi.org\/10.1109\/iac.2018.8780492","DOI":"10.1109\/IAC.2018.8780492"},{"key":"pone.0323847.ref057","doi-asserted-by":"crossref","unstructured":"Kolesnikov A, Beyer L, Zhai X, Puigcerver J, Yung J, Gelly S. Big Transfer (BiT): General visual representation learning. 2019. https:\/\/doi.org\/10.48550\/ARXIV.1912.11370","DOI":"10.1007\/978-3-030-58558-7_29"},{"key":"pone.0323847.ref058","doi-asserted-by":"crossref","unstructured":"Chollet F. Xception: Deep learning with depthwise separable convolutions. 2016. https:\/\/doi.org\/10.48550\/ARXIV.1610.02357","DOI":"10.1109\/CVPR.2017.195"},{"key":"pone.0323847.ref059","doi-asserted-by":"crossref","unstructured":"Huang G, Liu Z, van der Maaten L, Weinberger KQ. Densely connected convolutional networks. 2016. https:\/\/doi.org\/10.48550\/ARXIV.1608.06993","DOI":"10.1109\/CVPR.2017.243"},{"key":"pone.0323847.ref060","doi-asserted-by":"crossref","unstructured":"Szegedy C, Vanhoucke V, Ioffe S, Shlens J, Wojna Z. Rethinking the inception architecture for computer vision. 2015. https:\/\/doi.org\/10.48550\/ARXIV.1512.00567","DOI":"10.1109\/CVPR.2016.308"},{"key":"pone.0323847.ref061","first-page":"022022","article-title":"An overview of overfitting and its solutions","volume":"1168","author":"X Ying","year":"2019","journal-title":"J Phys: Conf Ser"},{"issue":"2","key":"pone.0323847.ref062","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1111\/j.2517-6161.1958.tb00292.x","article-title":"The regression analysis of binary sequences","volume":"20","author":"DR Cox","year":"1958","journal-title":"Journal of the Royal Statistical Society Series B: Statistical Methodology"},{"key":"pone.0323847.ref063","doi-asserted-by":"crossref","first-page":"4806","DOI":"10.1109\/ACCESS.2019.2962617","article-title":"The real-world-weight cross-entropy loss function: Modeling the costs of mislabeling","volume":"8","author":"Y Ho","year":"2020","journal-title":"IEEE Access"},{"key":"pone.0323847.ref064","doi-asserted-by":"crossref","unstructured":"Redmon J, Divvala S, Girshick R, Farhadi A. 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