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TMEM63 ion channels are reported to function as mechanosensors\/osmosensors in other organs, and our studies revealed that the primary site of\n                    <jats:italic>Tmem63a<\/jats:italic>\n                    and\n                    <jats:italic>Tmem63b<\/jats:italic>\n                    gene expression and TMEM63B protein expression in the mouse bladder wall was the urothelium. Despite this localization, voiding behavior in conditional urothelial\n                    <jats:italic>Tmem63b<\/jats:italic>\n                    knockout mice, assessed using a video-monitored void-spot screening assay, was not significantly different from control mice, even when the urothelium was stressed by exposure to cyclophosphamide. We further observed that dorsal root ganglia sensory neurons, including those innervating the bladder, were also sites of\n                    <jats:italic>Tmem63a<\/jats:italic>\n                    ,\n                    <jats:italic>Tmem63b<\/jats:italic>\n                    , and TMEM63B expression. Again, voiding behavior was not impacted in conditional sensory neuron\n                    <jats:italic>Tmem63b<\/jats:italic>\n                    knockout mice, treated or not with cyclophosphamide. Our studies reveal that the urothelium and dorsal root ganglia are sites of\n                    <jats:italic>Tmem63a<\/jats:italic>\n                    ,\n                    <jats:italic>Tmem63b<\/jats:italic>\n                    , and TMEM63B expression, but deletion of\n                    <jats:italic>Tmem63b<\/jats:italic>\n                    alone in these tissues does not result in a demonstrable voiding phenotype.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0324092","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T18:23:14Z","timestamp":1764958994000},"page":"e0324092","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Conditional deletion of Tmem63b does not impact mouse voiding behavior"],"prefix":"10.1371","volume":"20","author":[{"given":"Marianela 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