{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T02:17:34Z","timestamp":1792894654921,"version":"4.5.0"},"reference-count":62,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T00:00:00Z","timestamp":1747958400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","award":["HI22C1465"],"award-info":[{"award-number":["HI22C1465"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec017\">\n<jats:title>Background<\/jats:title>\n<jats:p>Obesity negatively affects reproduction and <jats:italic>in vitro<\/jats:italic> fertilization (IVF) outcomes. However, its effects on lipid metabolism during embryonic development remain unclear. We examined follicular fluid (FF) lipid composition and body mass index (BMI)-related embryological outcomes in Korean women undergoing IVF\/intracytoplasmic sperm injection (ICSI).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec018\">\n<jats:title>Methods<\/jats:title>\n<jats:p>This prospective cohort study included 68 Korean women with infertility without metabolic diseases who underwent IVF\/ICSI. Patients were categorized according to the 2022 guidelines of the Korean Society for the Study of Obesity as follows: Group A (obese, BMI\u2009\u2265\u200925\u2009kg\/m<jats:sup>2<\/jats:sup>, n\u2009=\u200928) and Group B (non-obese, BMI\u2009&lt;\u200925\u2009kg\/m<jats:sup>2<\/jats:sup>, n\u2009=\u200940). Liquid chromatography-tandem mass spectrometry (LC-MS) was used to analyze lipids in the FF. Principal component analysis (PCA) and correlation analyses were performed. Embryological outcomes according to the BMI were compared using the QUADE nonparametric analysis of covariance adjusted for age and anti-M\u00fcllerian hormone.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec019\">\n<jats:title>Results<\/jats:title>\n<jats:p>LC-MS identified 159 of the 230 lipids in the FF samples. Diacylglycerol (DAG), triacylglycerol (TAG), and acylcarnitine (AC) levels were significantly higher in the obese group; whereas monoacylglycerol (MAG) and plasmenyl phosphatidylcholine levels were lower. PCA explained 38.9% of the variance between the groups. Significant inter-group differences were found in the DAG (adjusted <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) and AC 16:1 (adjusted <jats:italic>p<\/jats:italic>\u2009=\u20090.0139) levels. BMI and TAG, DAG, and AC levels (adjusted <jats:italic>p\u2009<\/jats:italic>&lt;\u20090.05) were positively correlated. Obese group had fewer fertilized oocytes (5.07\u2009\u00b1\u20094.16 vs. 6.65\u2009\u00b1\u20094.61, <jats:italic>p\u2009<\/jats:italic>=\u20090.043), cleavage-stage embryos (4.86\u2009\u00b1\u20094.26 vs. 6.63\u2009\u00b1\u20094.61, <jats:italic>p\u2009<\/jats:italic>=\u20090.016), and morula-stage embryos (4.00\u2009\u00b1\u20094.51 vs. 6.05\u2009\u00b1\u20095.14, <jats:italic>p\u2009<\/jats:italic>=\u20090.024).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec020\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>Obesity alters FF lipid composition in women with infertility undergoing IVF\/ICSI, potentially affecting early embryonic development. This study improves our understanding of its effects on the ovarian microenvironment and offers insights into targeted IVF interventions.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0324511","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T17:53:41Z","timestamp":1748022821000},"page":"e0324511","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["Impact of obesity on follicular fluid lipid composition and IVF\/ICSI outcomes in Korean women: A lipidomic study"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0738-4153","authenticated-orcid":true,"given":"Yoo Ra","family":"Ko","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1753-2674","authenticated-orcid":true,"given":"Min Kyoung","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Won","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. Jung","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se Jeong","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Kyun","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"So Yeon","family":"Ahn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minseo","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surim","family":"Oh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sohyun","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donghyeon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2191-666X","authenticated-orcid":true,"given":"Hyunjung Jade","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Woo 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Si","year":"2021","journal-title":"Reprod Biol Endocrinol"},{"issue":"9933","key":"pone.0324511.ref013","doi-asserted-by":"crossref","first-page":"1990","DOI":"10.1016\/S0140-6736(13)62674-4","article-title":"DNA methylation and body-mass index: a genome-wide analysis","volume":"383","author":"KJ Dick","year":"2014","journal-title":"Lancet"},{"issue":"2","key":"pone.0324511.ref014","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1093\/humupd\/dmu060","article-title":"Obesity and the reproductive system disorders: epigenetics as a potential bridge","volume":"21","author":"AB Crujeiras","year":"2015","journal-title":"Hum Reprod Update"},{"key":"pone.0324511.ref015","doi-asserted-by":"crossref","first-page":"806890","DOI":"10.3389\/fcell.2022.806890","article-title":"Lipid metabolic process involved in oocyte maturation during folliculogenesis","volume":"10","author":"T Liu","year":"2022","journal-title":"Front Cell Dev 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Viardot-Foucault","year":"2023","journal-title":"J Ovarian Res"},{"issue":"1","key":"pone.0324511.ref022","doi-asserted-by":"crossref","DOI":"10.1530\/REP-13-0251","article-title":"Lipids and oocyte developmental competence: the role of fatty acids and \u03b2-oxidation","volume":"148","author":"KR Dunning","year":"2014","journal-title":"Reproduction"},{"issue":"3","key":"pone.0324511.ref023","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/S0015-0282(16)48440-2","article-title":"Lipoprotein and phospholipid distribution in human follicular fluids","volume":"43","author":"BP Perret","year":"1985","journal-title":"Fertil Steril"},{"issue":"5","key":"pone.0324511.ref024","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1210\/jc.2014-3649","article-title":"Follicular fluid concentrations of lipids and their metabolites are associated with intraovarian gonadotropin-stimulated androgen production in women undergoing in vitro fertilization","volume":"100","author":"A Gervais","year":"2015","journal-title":"J Clin Endocrinol Metab"},{"issue":"8","key":"pone.0324511.ref025","doi-asserted-by":"crossref","DOI":"10.3390\/biom10081135","article-title":"A shift in glycerolipid metabolism defines the follicular fluid of IVF patients with unexplained infertility","volume":"10","author":"A Batushansky","year":"2020","journal-title":"Biomolecules"},{"issue":"2","key":"pone.0324511.ref026","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.rbmo.2020.09.024","article-title":"Fatty acids of follicular fluid phospholipids and triglycerides display distinct association with IVF outcomes","volume":"42","author":"R Zarezadeh","year":"2021","journal-title":"Reprod Biomed Online"},{"issue":"1","key":"pone.0324511.ref027","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1186\/s12958-022-00974-7","article-title":"The fatty acid composition in follicles is related to the developmental potential of oocytes up to the blastocyst stage: a 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embryos","volume":"43","author":"K Ohata","year":"2021","journal-title":"Reprod Biomed Online"},{"issue":"2","key":"pone.0324511.ref039","doi-asserted-by":"crossref","first-page":"121","DOI":"10.7570\/jomes23031","article-title":"Diagnosis of obesity: 2022 update of clinical practice guidelines for obesity by the Korean society for the study of obesity","volume":"32","author":"J-H Haam","year":"2023","journal-title":"J Obes Metab Syndr"},{"issue":"5","key":"pone.0324511.ref040","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1194\/jlr.D700041-JLR200","article-title":"Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics","volume":"49","author":"V Matyash","year":"2008","journal-title":"J Lipid Res"},{"issue":"6","key":"pone.0324511.ref041","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1038\/nprot.2010.50","article-title":"Global metabolic profiling procedures for urine using UPLC-MS","volume":"5","author":"EJ Want","year":"2010","journal-title":"Nat Protoc"},{"key":"pone.0324511.ref042","doi-asserted-by":"crossref","first-page":"639704","DOI":"10.3389\/fcell.2021.639704","article-title":"Role of lipid metabolism and signaling in mammalian oocyte maturation, quality, and acquisition of competence","volume":"9","author":"R Khan","year":"2021","journal-title":"Front Cell Dev Biol"},{"issue":"20","key":"pone.0324511.ref043","doi-asserted-by":"crossref","DOI":"10.1242\/dev.202148","article-title":"Dynamic metabolism during early mammalian embryogenesis","volume":"150","author":"J Zhao","year":"2023","journal-title":"Development"},{"issue":"2","key":"pone.0324511.ref044","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1038\/s41556-023-01341-3","article-title":"Low-input lipidomics reveals lipid metabolism remodelling during early mammalian embryo development","volume":"26","author":"L Zhang","year":"2024","journal-title":"Nat Cell Biol"},{"issue":"5","key":"pone.0324511.ref045","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1016\/j.fertnstert.2012.01.122","article-title":"An investigation into the relationship between the metabolic profile of follicular fluid, oocyte developmental potential, and implantation outcome","volume":"97","author":"M Wallace","year":"2012","journal-title":"Fertil Steril"},{"issue":"4","key":"pone.0324511.ref046","doi-asserted-by":"crossref","DOI":"10.3390\/metabo10040147","article-title":"The lipolysome-a highly complex and dynamic protein network orchestrating cytoplasmic triacylglycerol degradation","volume":"10","author":"P Hofer","year":"2020","journal-title":"Metabolites"},{"key":"pone.0324511.ref047","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1146\/annurev.nutr.27.061406.093734","article-title":"Regulation of lipolysis in adipocytes","volume":"27","author":"RE Duncan","year":"2007","journal-title":"Annu Rev Nutr"},{"issue":"1","key":"pone.0324511.ref048","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1080\/19396368.2018.1516000","article-title":"Artificial oocyte activation: physiological, pathophysiological and ethical aspects","volume":"65","author":"G Anifandis","year":"2019","journal-title":"Syst Biol Reprod Med"},{"issue":"2","key":"pone.0324511.ref049","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1002\/mrd.21112","article-title":"Involvement of the PKC family in regulation of early development","volume":"77","author":"M Kalive","year":"2010","journal-title":"Mol Reprod Dev"},{"issue":"4","key":"pone.0324511.ref050","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1080\/15384101.2018.1564492","article-title":"PKC\u03b21 regulates meiotic cell cycle in mouse oocyte","volume":"18","author":"Z-Y Yi","year":"2019","journal-title":"Cell 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Chem"},{"issue":"35","key":"pone.0324511.ref054","doi-asserted-by":"crossref","first-page":"23761","DOI":"10.1016\/S0021-9258(18)54348-9","article-title":"Differential regulation of protein kinase C isozymes by thyrotropin-releasing hormone in GH4C1 cells","volume":"266","author":"SC Kiley","year":"1991","journal-title":"J Biol Chem"},{"issue":"4","key":"pone.0324511.ref055","doi-asserted-by":"crossref","first-page":"2758","DOI":"10.1016\/S0021-9258(17)42008-4","article-title":"Bombesin, platelet-derived growth factor, and diacylglycerol induce selective membrane association and down-regulation of protein kinase C isotypes in Swiss 3T3 cells","volume":"269","author":"AR Olivier","year":"1994","journal-title":"J Biol Chem"},{"issue":"4","key":"pone.0324511.ref056","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1038\/nm0410-400","article-title":"Diacylglycerol-mediated insulin resistance","volume":"16","author":"DM Erion","year":"2010","journal-title":"Nat 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