{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T02:24:50Z","timestamp":1792808690208,"version":"4.5.0"},"reference-count":61,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS KAKENHI","award":["23K09384"],"award-info":[{"award-number":["23K09384"]}]},{"name":"JSPS KAKENHI","award":["21K19617"],"award-info":[{"award-number":["21K19617"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>In tooth extraction sockets, natural healing is usually expected; however, early healing is advantageous for subsequent treatment and leads to improvement in the patient\u2019s quality of life. In recent years, it has been found that dental pulp tissue contains several dental pulp stem cells. The main objective of this study was to verify whether immediate transplantation of dental pulp tissue without any culture process into the extraction socket would promote healing of the socket. To verify this, the rat\u2019s bilateral maxillary second molars were extracted simultaneously, and dental pulp tissue was collected from the extracted teeth. The right extraction socket was immediately transplanted with dental pulp tissue, and the left extraction socket was used as a control. Both sockets were then covered with immediate curing resin. Micro-CT was performed over time, up to 28 days after tooth extraction, bone mineral content was measured, and histological examination was performed. As a result, inflammatory bone resorption had a normal course in both groups after tooth extraction, but bone resorption tended to be suppressed on the 3 days after tooth extraction in the transplant group. In the transplant group, compared to the control group, the radiopaque area increased from the 5 days after tooth extraction onward, and a significant difference was observed on the 7 days after tooth extraction. Furthermore, bone mineral content measurements suggested that bone maturation progressed from earlier in the transplant group, and histological examination confirmed that bone healing was promoted in the transplant group. These findings confirmed that immediate transplantation of dental pulp tissue can promote bone healing in the extraction socket and suggested that this method can be useful as a new method to promote healing in the extraction socket.<\/jats:p>","DOI":"10.1371\/journal.pone.0324536","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T17:42:44Z","timestamp":1751564564000},"page":"e0324536","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development of a new method to promote tooth extraction socket healing through immediate transplantation of extracted dental pulp tissue - An in vivo study"],"prefix":"10.1371","volume":"20","author":[{"given":"Kohei","family":"Ijichi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3932-5913","authenticated-orcid":true,"given":"Reiko","family":"Tokuyama-Toda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai","family":"Takeshita-Umehara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshio","family":"Yudo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Takebe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhito","family":"Satomura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,7,3]]},"reference":[{"issue":"3","key":"pone.0324536.ref001","article-title":"Effects of ozone therapy on postoperative pain, swelling, and trismus caused by surgical extraction of unerupted lower third molars: a double-blinded split-mouth randomized controlled trial","volume":"30","author":"R-B Almeida","year":"2025","journal-title":"Med Oral Patol Oral Cir Bucal"},{"issue":"1","key":"pone.0324536.ref002","article-title":"Evaluating the effectiveness of low-level laser therapy in patients undergoing lower third molar extraction: a double-blinded randomized controlled trial","volume":"30","author":"M-J Uzeda","year":"2025","journal-title":"Med Oral Patol Oral Cir Bucal"},{"issue":"4","key":"pone.0324536.ref003","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1016\/j.ijom.2016.11.006","article-title":"Effect of submucosal dexamethasone injection on postoperative pain, oedema, and trismus following mandibular third molar surgery: a prospective, randomized, double-blind clinical trial","volume":"46","author":"IM Mojsa","year":"2017","journal-title":"Int J Oral Maxillofac Surg"},{"issue":"3","key":"pone.0324536.ref004","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/0030-4220(69)90357-0","article-title":"The time sequence of tissue regeneration in human extraction wounds","volume":"27","author":"MH Amler","year":"1969","journal-title":"Oral Surg Oral Med Oral Pathol"},{"issue":"2","key":"pone.0324536.ref005","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1111\/j.1600-051X.2005.00642.x","article-title":"Dimensional ridge alterations following tooth extraction. An experimental study in the dog","volume":"32","author":"MG Ara\u00fajo","year":"2005","journal-title":"J Clin Periodontol"},{"issue":"1","key":"pone.0324536.ref006","first-page":"27","article-title":"Morphologic changes of the mandible after extraction and wearing of dentures. A longitudinal, clinical, and x-ray cephalometric study covering 5 years","volume":"18","author":"GE Carlsson","year":"1967","journal-title":"Odontol Revy"},{"issue":"5","key":"pone.0324536.ref007","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1016\/j.joms.2005.12.046","article-title":"Assessing postoperative discomfort after third molar surgery: a prospective study","volume":"65","author":"GB Grossi","year":"2007","journal-title":"J Oral Maxillofac Surg"},{"issue":"3","key":"pone.0324536.ref008","doi-asserted-by":"crossref","first-page":"510","DOI":"10.2319\/050814-332.1","article-title":"Early vs late orthodontic treatment of tooth crowding by first premolar extraction: a systematic review","volume":"85","author":"HL Filho","year":"2015","journal-title":"The Angle Orthodontist"},{"issue":"4","key":"pone.0324536.ref009","first-page":"393","article-title":"The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells","volume":"3","author":"AJ Friedenstein","year":"1970","journal-title":"Cell Tissue Kinet"},{"issue":"5","key":"pone.0324536.ref010","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1002\/jor.1100090504","article-title":"Mesenchymal stem cells","volume":"9","author":"AI Caplan","year":"1991","journal-title":"J Orthop Res"},{"issue":"2","key":"pone.0324536.ref011","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1089\/107632701300062859","article-title":"Multilineage cells from human adipose tissue: implications for cell-based therapies","volume":"7","author":"PA Zuk","year":"2001","journal-title":"Tissue Eng"},{"issue":"1","key":"pone.0324536.ref012","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1159\/000345615","article-title":"Introduction to stem cells and regenerative medicine","volume":"85","author":"G Kolios","year":"2013","journal-title":"Respiration"},{"key":"pone.0324536.ref013","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-3-030-31206-0_1","article-title":"Potential clinical applications of stem cells in regenerative medicine","volume":"1201","author":"S Suman","year":"2019","journal-title":"Adv Exp Med Biol"},{"issue":"1","key":"pone.0324536.ref014","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1111\/nyas.14243","article-title":"Adult stem cells and regenerative medicine-a symposium report","volume":"1462","author":"J Cable","year":"2020","journal-title":"Ann N Y Acad Sci"},{"issue":"25","key":"pone.0324536.ref015","doi-asserted-by":"crossref","first-page":"13625","DOI":"10.1073\/pnas.240309797","article-title":"Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo","volume":"97","author":"S Gronthos","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"10","key":"pone.0324536.ref016","doi-asserted-by":"crossref","first-page":"5807","DOI":"10.1073\/pnas.0937635100","article-title":"SHED: stem cells from human exfoliated deciduous teeth","volume":"100","author":"M Miura","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"4","key":"pone.0324536.ref017","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1016\/j.biotechadv.2018.03.011","article-title":"Stem cells: their source, potency and use in regenerative therapies with focus on adipose-derived stem cells - a review","volume":"36","author":"L Bacakova","year":"2018","journal-title":"Biotechnol Adv"},{"key":"pone.0324536.ref018","article-title":"The influence of Aloe vera with mesenchymal stem cells from dental pulp on bone regeneration: characterization and treatment of non-critical defects of the tibia in rats","volume":"27","author":"IMV Soares","year":"2019","journal-title":"J Appl Oral Sci"},{"key":"pone.0324536.ref019","doi-asserted-by":"crossref","first-page":"1339573","DOI":"10.3389\/fmed.2024.1339573","article-title":"Application of dental pulp stem cells for bone regeneration","volume":"11","author":"Y Liu","year":"2024","journal-title":"Front Med (Lausanne)"},{"issue":"03","key":"pone.0324536.ref020","doi-asserted-by":"crossref","first-page":"307","DOI":"10.4103\/1305-7456.137631","article-title":"Comparison of phenotype and differentiation marker gene expression profiles in human dental pulp and bone marrow mesenchymal stem cells","volume":"08","author":"D Ponnaiyan","year":"2014","journal-title":"Eur J Dent"},{"issue":"29","key":"pone.0324536.ref021","doi-asserted-by":"crossref","first-page":"6995","DOI":"10.1016\/j.biomaterials.2011.05.086","article-title":"A comparison between adipose tissue and dental pulp as sources of MSCs for tooth regeneration","volume":"32","author":"C-N Hung","year":"2011","journal-title":"Biomaterials"},{"issue":"1","key":"pone.0324536.ref022","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1186\/s13287-018-1094-8","article-title":"Recycle the dental fairy\u2019s package: overview of dental pulp stem cells","volume":"9","author":"X Yang","year":"2018","journal-title":"Stem Cell Res Ther"},{"issue":"4","key":"pone.0324536.ref023","doi-asserted-by":"crossref","first-page":"132","DOI":"10.14712\/fb2017063040132","article-title":"The differentiation potential of human natal dental pulp stem cells into insulin-producing cells","volume":"63","author":"J Suchanek","year":"2017","journal-title":"Folia Biol (Praha)"},{"key":"pone.0324536.ref024","first-page":"91","article-title":"Dental pulp stem cell (DPSC) isolation, characterization, and differentiation","volume-title":"Methods Mol Biol","author":"F Ferro","year":"2014"},{"issue":"5","key":"pone.0324536.ref025","first-page":"900","article-title":"Comparative study of differentiation potential of mesenchymal stem cells derived from orofacial system into vascular endothelial cells","volume":"51","author":"J Xie","year":"2019","journal-title":"Beijing Da Xue Xue Bao Yi Xue Ban"},{"key":"pone.0324536.ref026","first-page":"33","article-title":"Dental pulp stem cells: advances to applications","volume":"13","author":"TW Tsutsui","year":"2020","journal-title":"Stem Cells Cloning"},{"key":"pone.0324536.ref027","doi-asserted-by":"crossref","first-page":"5734539","DOI":"10.1155\/2020\/5734539","article-title":"Tissue engineering approaches for enamel, dentin, and pulp regeneration: an update","volume":"2020","author":"GM Ahmed","year":"2020","journal-title":"Stem Cells Int"},{"issue":"1","key":"pone.0324536.ref028","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.bbrc.2018.04.213","article-title":"Comparative characterization of stem cells from human exfoliated deciduous teeth, dental pulp, and bone marrow-derived mesenchymal stem cells","volume":"501","author":"R Kunimatsu","year":"2018","journal-title":"Biochem Biophys Res Commun"},{"key":"pone.0324536.ref029","doi-asserted-by":"crossref","DOI":"10.1590\/1678-7757-2017-0326","article-title":"Alveolar bone healing in rats: micro-CT, immunohistochemical and molecular analysis","volume":"26","author":"JS Hassumi","year":"2018","journal-title":"J Appl Oral Sci"},{"issue":"16","key":"pone.0324536.ref030","doi-asserted-by":"crossref","first-page":"4457","DOI":"10.3390\/ma14164457","article-title":"Replacement process of carbonate apatite by alveolar bone in a rat extraction socket","volume":"14","author":"X Zhang","year":"2021","journal-title":"Materials (Basel)"},{"issue":"2","key":"pone.0324536.ref031","article-title":"The influence of alendronate on the healing of extraction sockets of ovariectomized rats assessed by in vivo micro-computed tomography","volume":"110","author":"J-H Jee","year":"2010","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endod"},{"issue":"4","key":"pone.0324536.ref032","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1016\/j.bone.2009.12.016","article-title":"Osteocalcin immunolabeling during the alveolar healing process in ovariectomized rats treated with estrogen or raloxifene","volume":"46","author":"ER Luvizuto","year":"2010","journal-title":"Bone"},{"issue":"1","key":"pone.0324536.ref033","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.archoralbio.2009.11.001","article-title":"Histomorphometric analysis and immunolocalization of RANKL and OPG during the alveolar healing process in female ovariectomized rats treated with oestrogen or raloxifene","volume":"55","author":"ER Luvizuto","year":"2010","journal-title":"Arch Oral Biol"},{"issue":"2","key":"pone.0324536.ref034","first-page":"153","article-title":"Wound healing following tooth extraction. Histochemical study in rats","volume":"2","author":"T Okamoto","year":"1973","journal-title":"Rev Fac Odontol Aracatuba"},{"issue":"1","key":"pone.0324536.ref035","first-page":"63","article-title":"Comparative histological study of two methods of obtaining alveolar sections in rats","volume":"19","author":"T Okamoto","year":"1990","journal-title":"Rev Odontol UNESP"},{"issue":"3","key":"pone.0324536.ref036","doi-asserted-by":"crossref","first-page":"430","DOI":"10.4012\/dmj.2023-262","article-title":"Detection of senescent cells in the mucosal healing process on type 2 diabetic rats after tooth extraction for biomaterial development","volume":"43","author":"C Luo","year":"2024","journal-title":"Dent Mater J"},{"key":"pone.0324536.ref037","first-page":"908098","article-title":"Impact of ellagic acid in bone formation after tooth extraction: an experimental study on diabetic rats","volume":"2014","author":"MMJ Al-Obaidi","year":"2014","journal-title":"Scient WorldJ"},{"key":"pone.0324536.ref038","doi-asserted-by":"crossref","first-page":"107772","DOI":"10.1016\/j.intimp.2021.107772","article-title":"Malnutrition delayed wound healing after tooth extraction by HMGB1-related prolonged inflammation","volume":"96","author":"Y Zhang","year":"2021","journal-title":"Int Immunopharmacol"},{"issue":"1","key":"pone.0324536.ref039","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1186\/s12903-024-04127-7","article-title":"Artesunate-loaded thermosensitive chitosan hydrogel promotes osteogenesis of maxillary tooth extraction through regulating T lymphocytes in type 2 diabetic rats","volume":"24","author":"J Luo","year":"2024","journal-title":"BMC Oral Health"},{"issue":"11","key":"pone.0324536.ref040","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.3390\/bioengineering11111057","article-title":"Tetracalcium Phosphate\/Monetite\/Calcium sulfate hemihydrate biocement for alveolar bone augmentation after tooth extraction in pig mandible","volume":"11","author":"K Vdoviakov\u00e1","year":"2024","journal-title":"Bioengineering (Basel)"},{"issue":"9","key":"pone.0324536.ref041","first-page":"577","article-title":"Comparative investigation of photobiomodulation in diabetes-impaired alveolar bone healing: a histomorphometrical and molecular study","volume":"42","author":"Z Dalirsani","year":"2024","journal-title":"Photobiomodul Photomed Laser Surg"},{"issue":"3","key":"pone.0324536.ref042","doi-asserted-by":"crossref","DOI":"10.3892\/etm.2023.11803","article-title":"G\u2011CSF delays tooth extraction socket bone healing via the inhibition of bone turnover in mice","volume":"25","author":"M Oshitani","year":"2023","journal-title":"Exp Ther Med"},{"issue":"18","key":"pone.0324536.ref043","doi-asserted-by":"crossref","first-page":"6850","DOI":"10.3390\/ijms21186850","article-title":"Wound healing and cell dynamics including mesenchymal and dental pulp stem cells induced by photobiomodulation therapy: an example of socket-preserving effects after tooth extraction in rats and a literature review","volume":"21","author":"Y Daigo","year":"2020","journal-title":"Int J Mol Sci"},{"issue":"1","key":"pone.0324536.ref044","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1186\/s40064-016-2263-9","article-title":"Tooth replantation with adipose tissue stem cells and fibrin sealant: microscopic analysis of rat\u2019s teeth","volume":"5","author":"S Demirel","year":"2016","journal-title":"Springerplus"},{"issue":"2","key":"pone.0324536.ref045","doi-asserted-by":"crossref","first-page":"284","DOI":"10.2334\/josnusd.18-0143","article-title":"Bone marrow-derived mesenchymal stem cells enhance bone marrow regeneration in dental extraction sockets","volume":"61","author":"T Mashimo","year":"2019","journal-title":"J Oral Sci"},{"issue":"1","key":"pone.0324536.ref046","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1186\/s13287-019-1277-y","article-title":"Adipose-derived stem cells prevent the onset of bisphosphonate-related osteonecrosis of the jaw through transforming growth factor \u03b2-1-mediated gingival wound healing","volume":"10","author":"X Zang","year":"2019","journal-title":"Stem Cell Res Ther"},{"key":"pone.0324536.ref047","doi-asserted-by":"crossref","first-page":"119480","DOI":"10.1016\/j.lfs.2021.119480","article-title":"Transplantation of bone marrow mesenchymal stem cells and fibrin glue into extraction socket in maxilla promoted bone regeneration in osteoporosis rat","volume":"290","author":"Q Niu","year":"2022","journal-title":"Life Sci"},{"issue":"4","key":"pone.0324536.ref048","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1007\/s10561-015-9498-5","article-title":"Effect of uncontrolled freezing on biological characteristics of human dental pulp stem cells","volume":"16","author":"A Kumar","year":"2015","journal-title":"Cell Tissue Bank"},{"key":"pone.0324536.ref049","doi-asserted-by":"crossref","first-page":"101819","DOI":"10.1016\/j.tice.2022.101819","article-title":"Long-term hypoxia inhibits the passage-dependent stemness decrease and senescence increase of human dental pulp stem cells","volume":"76","author":"H Meng","year":"2022","journal-title":"Tissue Cell"},{"issue":"1","key":"pone.0324536.ref050","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1007\/s10561-021-09926-8","article-title":"Comparative evaluation of proliferative potential and replicative senescence associated changes in mesenchymal stem cells derived from dental pulp and umbilical cord","volume":"23","author":"M Das","year":"2022","journal-title":"Cell Tissue Bank"},{"issue":"1","key":"pone.0324536.ref051","doi-asserted-by":"crossref","DOI":"10.1186\/s13287-017-0705-0","article-title":"Isolation and prolonged expansion of oral mesenchymal stem cells under clinical-grade, GMP-compliant conditions differentially affects \u201cstemness\u201d properties","volume":"8","author":"A Bakopoulou","year":"2017","journal-title":"Stem Cell Res Ther"},{"issue":"1","key":"pone.0324536.ref052","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1159\/000448026","article-title":"Analysis of senescence-related differentiation potentials and gene expression profiles in human dental pulp stem cells","volume":"203","author":"Q Yi","year":"2016","journal-title":"Cells Tissues Organs"},{"key":"pone.0324536.ref053","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.archoralbio.2017.09.002","article-title":"Simplified conditions for storing and cryopreservation of dental pulp stem cells","volume":"84","author":"NC-N Huynh","year":"2017","journal-title":"Arch Oral Biol"},{"issue":"5","key":"pone.0324536.ref054","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.3390\/biomedicines10051049","article-title":"The therapeutic potential of secreted factors from dental pulp stem cells for various diseases","volume":"10","author":"K Ogata","year":"2022","journal-title":"Biomedicines"},{"key":"pone.0324536.ref055","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.jfma.2018.08.003","article-title":"Pure type-1 collagen application to third molar extraction socket reduces postoperative pain score and duration and promotes socket bone healing","volume":"118","author":"S-J Tsai","year":"2019","journal-title":"J Formos Med Assoc"},{"issue":"12","key":"pone.0324536.ref056","doi-asserted-by":"crossref","first-page":"1738","DOI":"10.3390\/biom12121738","article-title":"Histological and immunohistochemical analysis of the effects of topical melatonin treatment associated with collagen sponge and rhBMP-2 protein on bone remodeling","volume":"12","author":"LF Montarele","year":"2022","journal-title":"Biomolecules"},{"issue":"2","key":"pone.0324536.ref057","first-page":"31","article-title":"Evaluation of the effects of melatonin and vitamin D on wound healing in immunosuppressive rats following tooth extraction","volume":"25","author":"D Celik","year":"2023","journal-title":"Stomatologija"},{"issue":"6","key":"pone.0324536.ref058","article-title":"Effects of local melatonin application on post-extraction sockets after third molar surgery. A pilot study","volume":"19","author":"C Cobo-V\u00e1zquez","year":"2014","journal-title":"Med Oral Patol Oral Cir Bucal"},{"issue":"8","key":"pone.0324536.ref059","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.kjms.2016.06.005","article-title":"Therapeutic potential of melatonin in oral medicine and periodontology","volume":"32","author":"S Najeeb","year":"2016","journal-title":"Kaohsiung J Med Sci"},{"issue":"1","key":"pone.0324536.ref060","article-title":"Melatonin enhances vertical bone augmentation in rat calvaria secluded spaces","volume":"21","author":"H Shino","year":"2016","journal-title":"Med Oral Patol Oral Cir Bucal"},{"issue":"5","key":"pone.0324536.ref061","doi-asserted-by":"crossref","first-page":"10063","DOI":"10.3390\/ijms140510063","article-title":"Melatonin effects on hard tissues: bone and tooth","volume":"14","author":"J Liu","year":"2013","journal-title":"Int J Mol Sci"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0324536","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T17:42:58Z","timestamp":1751564578000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0324536"}},"subtitle":[],"editor":[{"given":"Carlos Alberto Antunes","family":"Viegas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,7,3]]},"references-count":61,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7,3]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0324536","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,3]]}}}