{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T11:22:05Z","timestamp":1784287325421,"version":"3.55.0"},"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"USDA National Institute of Food and Agriculture multi-state project","award":["ILLU-698-981"],"award-info":[{"award-number":["ILLU-698-981"]}]},{"name":"University of Illinois at Urbana-Champaign, Urbana","award":["IL 61801"],"award-info":[{"award-number":["IL 61801"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Whole genome sequences (WGSs) of\n                    <jats:italic>\n                      <jats:italic>Enterococcus fa<\/jats:italic>\n                      ecalis\n                    <\/jats:italic>\n                    S3 and\n                    <jats:italic>\n                      S\n                      <jats:italic>taphylococ<\/jats:italic>\n                      cus haemolyticus\n                    <\/jats:italic>\n                    S5, isolated from gymnasium equipment in Tennessee, USA, were analyzed. The genome sizes of\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    S3 and\n                    <jats:italic>S. haemolyticus<\/jats:italic>\n                    S5 were approximately 3.0\u2009Mb and 2.5\u2009Mb, respectively. Both isolates were found to harbor genes conferring resistance to multiple antibiotics, including tetracycline, fluoroquinolone, and macrolide. Gene cluster analysis revealed a cyclic lactone inducer cluster in both strains, which is critical for quorum sensing-mediated pathogenicity. Multilocus sequence typing (MLST) identified\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    S3 as ST40 and\n                    <jats:italic>S. haemolyticus<\/jats:italic>\n                    S5 as ST52. Notably, evolutionary analysis of gene contraction and expansion in these isolates revealed an expansion of genes associated with horizontal gene transfer. This expansion likely represents an evolutionary strategy to facilitate the spread of antibiotic resistance genes to other isolates. These findings offer valuable insights into the genomic apparatus responsible for antibiotic resistance and potential transmission mechanisms in human-associated environments.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0324894","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T17:29:25Z","timestamp":1761758965000},"page":"e0324894","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Whole genome sequence of multidrug-resistant Staphylococcus haemolyticus and Enterococcus faecalis isolates from public gymnasium equipment reveals evolving infection potential and resistance"],"prefix":"10.1371","volume":"20","author":[{"given":"Goutam","family":"Banerjee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5553-5350","authenticated-orcid":true,"given":"Pratik","family":"Banerjee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,10,29]]},"reference":[{"issue":"10325","key":"pone.0324894.ref001","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis.","volume":"399","author":"CJL Murray","year":"2022","journal-title":"Lancet"},{"key":"pone.0324894.ref002","volume-title":"Antimicrobial resistance: global report on surveillance","author":"WHO","year":"2014"},{"key":"pone.0324894.ref003","unstructured":"WHO. 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