{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:02:55Z","timestamp":1787022175410,"version":"build-2736575974"},"reference-count":17,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T00:00:00Z","timestamp":1750636800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["408214\/2021-0"],"award-info":[{"award-number":["408214\/2021-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"name":"CAPES (Foundation Coordination for the Improvement of Higher Education Personnel), INCT Oral Health and Dentistry","award":["406840\/2022-9"],"award-info":[{"award-number":["406840\/2022-9"]}]},{"DOI":"10.13039\/501100004901","name":"FAPEMIG","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec>\n                    <jats:title>Statement of the problem<\/jats:title>\n                    <jats:p>It is not clear the effect of multiple autoclaving processes on the accuracy of scan bodies. Autoclaving dental disposables enables reuse, reducing the environmental impact of raw materials and waste.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>This in vitro laboratory study evaluated the effect of the autoclaving process on the intraoral scan body (SB) manufactured from different materials (Titanium and PEEK), positioned at the implant and abutment levels.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Two models, each with 10 implants, were created to evaluate scan body trueness made of two materials (Titanium and PEEK), and implant junction positioned at two levels (implant and abutment levels). Analyses were conducted at five distinct time points: T0 (control group with new SB), T1 (after one autoclaving cycle), T2 (10 cycles), T3 (50 cycles), and T4 (100 cycles). Forty SBs were divided into four groups (n = 10), fixed on the models according to the initial positioning, using the recommended torque, and scanned 10 times using an intraoral Sccaner (InEOS X5 scanner, Dentsply Sirona). The surface deviation was evaluated by .STL mesh overlay using an image software (Geomagic Control X software). Microscopic analyses of marginal adaptation were performed simultaneously with the digital analysis using an optical microscope (Mitutoyo\u2122-500 Optical Microscope) with 40x magnification at four places (mesial, distal, buccal, and lingual). Statistical analysis was performed using statistical software (SPSS 29.0 software), with \u03b1 = 0,05. Data were subjected to the Shapiro-Wilk normality test. To assess spatial variation after the cycles, Repeated Measures ANOVA with Bonferroni correction was used. For microscopic qualitative analysis, percentile was used to check the prevalence of clinically adapted and misfitting faces.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Statistically significant differences in mesh deformation were detected, with PEEK SB showing greater deformation compared to Titanium, especially at the abutment level, after 100 cycles (P &lt; 0.01). Microscopic analysis revealed that 100% of the faces were classified as \u201cClinically Adapted\u201d by the three calibrated evaluators (KAPPA &gt; 0.08).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Titanium scan bodies showed less surface deviation than PEEK but both remained below 50 \u00b5m. Internal-junction scan bodies were more affected than abutment-level ones. All scan bodies stayed within acceptable limits and viable after 100 autoclaving cycles.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0325068","type":"journal-article","created":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T13:42:15Z","timestamp":1750686135000},"page":"e0325068","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of autoclaving process on trueness and qualitative marginal fit of intraoral scan body manufactured from two materials: An in vitro study"],"prefix":"10.1371","volume":"20","author":[{"given":"F\u00e1bio Henrique de Paulo","family":"Costa Santos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4201-4059","authenticated-orcid":true,"given":"Patr\u00edcia Santos","family":"de Melo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo S\u00e9rgio","family":"Borella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1vio Domingues das","family":"Neves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2205-9842","authenticated-orcid":true,"given":"Karla","family":"Zancope","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,6,23]]},"reference":[{"issue":"1","key":"pone.0325068.ref001","doi-asserted-by":"crossref","first-page":"52","DOI":"10.4012\/dmj.2019-224","article-title":"Progress in digital dentistry: The practical use of intraoral scanners","volume":"39","author":"K Suese","year":"2020","journal-title":"Dent Mater J"},{"issue":"8","key":"pone.0325068.ref002","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.3889\/oamjms.2018.308","article-title":"Advantages of CAD\/CAM versus Conventional Complete Dentures - A Review","volume":"6","author":"NM Janeva","year":"2018","journal-title":"Open Access Maced J Med Sci"},{"issue":"2","key":"pone.0325068.ref003","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.jpor.2019.06.001","article-title":"Influence of implant scanbody material, position and operator on the accuracy of digital impression for complete-arch: A randomized in vitro trial","volume":"64","author":"L Arcuri","year":"2020","journal-title":"J Prosthodont Res"},{"issue":"1","key":"pone.0325068.ref004","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/s40729-023-00517-8","article-title":"Accuracy of digital implant impressions obtained using intraoral scanners: a systematic review and meta-analysis of in vivo studies","volume":"9","author":"J Ma","year":"2023","journal-title":"Int J Implant Dent"},{"issue":"12","key":"pone.0325068.ref005","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0295790","article-title":"Influence of scanbody design and intraoral scanner on the trueness of complete arch implant digital impressions: An in vitro study","volume":"18","author":"PC Meneghetti","year":"2023","journal-title":"PLoS One"},{"issue":"4","key":"pone.0325068.ref006","first-page":"414","article-title":"Validation of the sterilization procedure of medical and hospital devices according to different packaging types","volume":"55","author":"FP Brito M de","year":"2002","journal-title":"Rev Bras Enferm"},{"issue":"4","key":"pone.0325068.ref007","first-page":"352","article-title":"Contemporary Sterilization Protocols of Healing Abutments for Reusability: A Systematic Review","volume":"7","author":"P Eswaramurthy","year":"2022","journal-title":"JDR Clin Trans Res"},{"issue":"21","key":"pone.0325068.ref008","doi-asserted-by":"crossref","first-page":"7717","DOI":"10.3390\/ma15217717","article-title":"Effects of Autoclave Sterilization and Multiple Use on Implant Scanbody Deformation In Vitro","volume":"15","author":"T Kato","year":"2022","journal-title":"Materials (Basel)"},{"issue":"1","key":"pone.0325068.ref009","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s40729-024-00543-0","article-title":"A systematic review of factors impacting intraoral scanning accuracy in implant dentistry with emphasis on scan bodies","volume":"10","author":"P Gehrke","year":"2024","journal-title":"Int J Implant Dent"},{"issue":"2","key":"pone.0325068.ref010","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1111\/cid.13187","article-title":"Effect of material type, torque value, and sterilization on linear displacements of a scan body: An in vitro study","volume":"25","author":"E Diker","year":"2023","journal-title":"Clin Implant Dent Relat Res"},{"key":"pone.0325068.ref011","volume-title":"Health and the Sustainable Development Goals","author":"World Health Organization","year":"2015"},{"issue":"1","key":"pone.0325068.ref012","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.prosdent.2016.09.024","article-title":"Evaluation of the accuracy of 7 digital scanners: An in vitro analysis based on 3-dimensional comparisons","volume":"118","author":"W Renne","year":"2017","journal-title":"J Prosthet Dent"},{"issue":"6","key":"pone.0325068.ref013","doi-asserted-by":"crossref","first-page":"785","DOI":"10.3390\/jcm8060785","article-title":"A Comparison Study of Marginal and Internal Fit Assessment Methods for Fixed Dental Prostheses","volume":"8","author":"K Son","year":"2019","journal-title":"J Clin Med"},{"issue":"2","key":"pone.0325068.ref014","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1515\/ijb-2023-0037","article-title":"Statistical models for assessing agreement for quantitative data with heterogeneous random raters and replicate measurements","volume":"20","author":"CT Ekstr\u00f8m","year":"2024","journal-title":"Int J Biostat"},{"issue":"2","key":"pone.0325068.ref015","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.prosdent.2019.12.011","article-title":"Influence of operator experience, scanner type, and scan size on 3D scans","volume":"125","author":"CCD Resende","year":"2021","journal-title":"J Prosthet Dent"},{"issue":"6","key":"pone.0325068.ref016","first-page":"1045","article-title":"The effect of different screw-tightening techniques on the stress generated on an internal-connection implant superstructure","volume":"24","author":"J-H Choi","year":"2009","journal-title":"Int J Oral Maxillofac Implants"},{"key":"pone.0325068.ref017","volume-title":"Instructions for the use of the intraoral scan body. Code 330306. 2023","author":"NEODENT","year":"2021"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0325068","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T13:42:20Z","timestamp":1750686140000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0325068"}},"subtitle":[],"editor":[{"given":"Antonio","family":"Riveiro Rodr\u00edguez,","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,6,23]]},"references-count":17,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2025,6,23]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0325068","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,23]]}}}