{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T11:08:50Z","timestamp":1788779330122,"version":"build-2803163510"},"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T00:00:00Z","timestamp":1749081600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec>\n<jats:title>Background\/aims<\/jats:title>\n<jats:p>Ulcerative colitis (UC) is a chronic inflammatory disease affecting the colon. Mesenchymal stem cells (MSCs) are candidates for use in inflammatory diseases with their tissue regeneration and anti-inflammatory capabilities. Chemokine receptor overexpression on MSCs is an effective strategy for the migration of MSCs into inflammatory tissue in high amounts. In this study, the anti-inflammatory and regenerative effects of genetically CCR2 overexpressed gingiva MSCs (GMSCs) on the inflamed colon in UC were studied.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Materials and methods<\/jats:title>\n<jats:p>GMSCs were transduced with a lentiviral vector for CCR2 overexpression. The UC experimental model was induced with a single intrarectal administration of 4% w\/w acetic acid. Colon tissues were analyzed for IFN or IL-17 secreting CD4+ \u2009T lymphocytes via flow cytometry and lymphocytic infiltration, fibrosis, and ulcers by histopathologic evaluation. Homing analysis of GMSCs was done by analyzing fluorescence intensity under the fluorescence microscope.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>GMSCs and CCR2<jats:sup>+<\/jats:sup>GMSCs equally downregulated colonic IFN-\u03b3 or IL-17 secreting CD4+ T lymphocytes ratios compared to the untreated group (p\u2009&lt;\u20090.05). Fluorescence intensity of labeled cells was significantly high in colon tissues of CCR2<jats:sup>+<\/jats:sup>GMSCs administered rats (61.2\u2009\u00b1\u200913.7%) compared to GMSCs administered rats (19.6\u2009\u00b1\u20099.8%) (p\u2009&lt;\u20090.05). In addition, mucosal integrity significantly increased and fibrosis and ulcers notably decreased in CCR2<jats:sup>+<\/jats:sup>GMSCs administered rats compared to GMSCs administered rats (p\u2009&lt;\u20090.05).<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion<\/jats:title>\n<jats:p>The overexpression of CCR2 on GMSCs increases migration to the inflammatory colon tissue, which has a high regenerative effect in UC. Overexpression of CCR2 on GMSCs may be an alternative to cellular therapies in UC.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0325566","type":"journal-article","created":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T17:53:26Z","timestamp":1749146006000},"page":"e0325566","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["CCR2 overexpressing gingiva mesenchymal stem cells provide high intestinal regeneration in a rat model of ulcerative 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