{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T10:52:36Z","timestamp":1784976756724,"version":"3.55.0"},"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T00:00:00Z","timestamp":1752451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"International Congress of Oral Implantologists"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Limited clinical data exists on the peri-implant microbiome in edentulous patients with implant-retained overdentures (IRO). This study aimed to examine the peri-implant microbiome and its correlation with pre-treatment periodontal diagnosis. A total of twenty-five patients with 50 implants were included, with demographic information and pre-treatment periodontal diagnosis collected. Clinical measurements and subgingival plaque samples were obtained from each implant, followed by 16S rRNA gene-targeted sequencing. Peri-implant parameters and microbiome were analyzed in relation to gender, remaining teeth prior to treatment, IRO function time, implant system, bone graft at implant placement, and peri-implant diagnosis. The mean age of patients was 71 years, with pre-extraction diagnoses including clinical healthy gingiva on a reduced periodontium (4 patients), localized periodontitis (4 patients), generalized periodontitis (7 patients), and generalized Stage IV Grade C periodontitis (10 patients). Nineteen implants were diagnosed with peri-implant mucositis, while 31 were healthy. The predominant genera detected were <jats:italic>Streptococcus<\/jats:italic>, <jats:italic>Rothia<\/jats:italic>, <jats:italic>Veillonella<\/jats:italic>, <jats:italic>Actinomyces<\/jats:italic>, and <jats:italic>Schaalia<\/jats:italic>. No significant correlation was found between pre-treatment periodontal diagnosis and peri-implant diagnosis. The findings suggest that while pre-extraction periodontal diagnosis might influence the peri-implant subgingival microbiome in IRO patients, it may not correlate with the clinical peri-implant diagnosis.<\/jats:p>","DOI":"10.1371\/journal.pone.0325711","type":"journal-article","created":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T17:30:56Z","timestamp":1752514256000},"page":"e0325711","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Correlation between pre-extraction periodontal diagnosis and peri-implant microbiome for patients treated with implant-retained overdenture \u2013 A retrospective cohort pilot study"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1954-6404","authenticated-orcid":true,"given":"Xiaoxi","family":"Cui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Acela A.","family":"Martinez Luna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Gillone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud","family":"Serag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8478-6981","authenticated-orcid":true,"given":"Ramiro Mendonca","family":"Murata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,7,14]]},"reference":[{"key":"pone.0325711.ref001","doi-asserted-by":"crossref","DOI":"10.1590\/1807-3107bor-2019.vol33.0063","article-title":"Prevalence of peri-implant diseases - a critical review on the current evidence","volume":"33","author":"R Cosgarea","year":"2019","journal-title":"Braz Oral Res"},{"key":"pone.0325711.ref002","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jdent.2017.04.011","article-title":"Prevalences of peri-implantitis and peri-implant mucositis: systematic review and meta-analysis","volume":"62","author":"C-T Lee","year":"2017","journal-title":"J Dent"},{"issue":"3","key":"pone.0325711.ref003","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1902\/jop.2014.140438","article-title":"Prevalence and predictive factors for peri-implant disease and implant failure: a cross-sectional analysis","volume":"86","author":"DM Daubert","year":"2015","journal-title":"J Periodontol"},{"key":"pone.0325711.ref004","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1111\/j.1600-051X.2010.01666.x","article-title":"The characteristics of biofilms in peri-implant disease","volume":"38","author":"A Mombelli","year":"2011","journal-title":"J Clin Periodontol"},{"key":"pone.0325711.ref005","doi-asserted-by":"crossref","first-page":"104776","DOI":"10.1016\/j.archoralbio.2020.104776","article-title":"A systematic review of the microbiota composition in various peri-implant conditions: data from 16S rRNA gene sequencing","volume":"117","author":"F de Melo","year":"2020","journal-title":"Arch Oral Biol"},{"key":"pone.0325711.ref006","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1111\/j.1600-051X.2008.01283.x","article-title":"Peri-implant diseases: Consensus Report of the Sixth European Workshop on Periodontology","volume":"35","author":"Group D of European Workshop on Periodontology","year":"2008","journal-title":"J Clin Periodontol"},{"key":"pone.0325711.ref007","article-title":"Peri-implantitis","author":"F Schwarz","year":"2018","journal-title":"J Clin Periodontol"},{"issue":"1","key":"pone.0325711.ref008","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1111\/prd.12008","article-title":"Surgical and nonsurgical periodontal therapy. 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