{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T21:06:05Z","timestamp":1791839165167,"version":"4.3.4"},"reference-count":73,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T00:00:00Z","timestamp":1750118400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Antimicrobial resistance (AMR) poses a growing threat to global health, with wound infections serving as significant reservoirs for multidrug-resistant bacteria. Understanding the socio-demographic, clinical, and microbiological characteristics of wound infections is critical for effective intervention strategies in resource-limited settings. Thus, this study aimed to evaluate the diversity, bacterial profiles, and resistance patterns of wound infections at Kisii Teaching and Referral Hospital. To do this, a total of 118 bacterial isolates from wound swabs were collected from 149 patients who met the eligibility criteria, from May to November 2024. These samples were cultured and analysed using standard microbiological techniques and antibiotic susceptibility testing according to the Clinical and Laboratory Standards Institute guidelines. Resistance genes associated with Vancomycin resistance in <jats:italic>Staphylococcus aureus<\/jats:italic> and <jats:italic>Staphylococcus xylosus<\/jats:italic> were also investigated using polymerase chain reaction (PCR) with appropriate primers and agarose gel electrophoresis. The results revealed a predominance of male patients (n\u2009=\u200979; 53.02%), avulsions (n\u2009=\u200950; 34.00%), and wounds on lower limbs (n\u2009=\u200963; 42.29%). <jats:italic>Escherichia coli<\/jats:italic> (n\u2009=\u200933; 27.97%), <jats:italic>Enterobacter gergoviae<\/jats:italic> (n\u2009=\u200920; 16.95%), <jats:italic>Staphylococcus aureus<\/jats:italic> (n\u2009=\u200918; 15.25%), and <jats:italic>Staphylococcus xylosus<\/jats:italic> (n\u2009=\u200918; 15.25%) were the most frequently isolated pathogens. Alarmingly high resistance rates were observed against ACCESS antibiotics, including Cotrimoxazole-Trimethoprim (n\u2009=\u2009114; 97%), Ampicillin (n\u2009=\u2009109; 92%), and Streptomycin (n\u2009=\u2009100; 85%). Resistance to Watch and Reserve antibiotics, including Vancomycin (n\u2009=\u200911; 29%) and Ceftriaxone (n\u2009=\u200927; 33%), was also documented, particularly among Gram-positive isolates such as <jats:italic>Staphylococcus aureus<\/jats:italic> and Gram-negative pathogens like <jats:italic>Pseudomonas aeruginosa<\/jats:italic>. Resistance genes (<jats:italic>Van<\/jats:italic>A, <jats:italic>Van<\/jats:italic>B, <jats:italic>Van<\/jats:italic>C) were detected in <jats:italic>S. aureus<\/jats:italic> and <jats:italic>S. xylosus<\/jats:italic>, underscoring the role of genetic mechanisms in resistance propagation. These findings highlight the critical need for targeted antimicrobial stewardship programmes and strengthened infection prevention measures in high-burden hospital settings, such as Kisii Teaching and Referral Hospital. This study underscores the importance of local resistance data in guiding empirical therapy and informs policies to mitigate AMR in resource-limited contexts. Moreover, enhanced surveillance and research into alternative therapies are crucial to safeguarding the efficacy of life-saving antibiotics and improving patient care outcomes.<\/jats:p>","DOI":"10.1371\/journal.pone.0326048","type":"journal-article","created":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T17:37:57Z","timestamp":1750181877000},"page":"e0326048","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Diversity and antibiotic susceptibility profiles of bacterial isolates from wound infections in patients at the surgical unit of Kisii teaching and referral hospital, Kenya"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-3616-8277","authenticated-orcid":true,"given":"Samson","family":"Onsando","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wycliffe","family":"Masanta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Nyerere","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moses","family":"Njire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5604-9987","authenticated-orcid":true,"given":"Gervason","family":"Moriasi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,6,17]]},"reference":[{"key":"pone.0326048.ref001","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.jgar.2021.05.010","article-title":"Challenges 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