{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T09:14:54Z","timestamp":1782206094057,"version":"3.54.5"},"reference-count":54,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T00:00:00Z","timestamp":1750896000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ratchadapisek Somphot Endowment Fund","award":["RA 66\/002"],"award-info":[{"award-number":["RA 66\/002"]}]},{"name":"Ratchadapisek Somphot Fund for Postdoctoral Fellowship"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Ivermectin (IVM), an antiparasitic drug, has been explored for its anticancer properties in various cancer types, including breast cancer. Endocrine therapy resistance poses a significant challenge in breast cancer treatment, often leading to metastasis prevention failure. This study aimed to investigate the effects of IVM on endocrine-resistant breast cancer cells, focusing on mechanisms associated with epithelial-to-mesenchymal transition (EMT). IVM was administered to endocrine-resistant breast cancer cell lines, MCF-7\/LCC2 (tamoxifen resistant) and MCF-7\/LCC9 (fulvestrant resistant), to evaluate its influence on cell proliferation, invasion, and EMT-related mechanisms. The findings indicated that IVM\u2019s half-maximum inhibitory concentration (IC<jats:sub>50<\/jats:sub>) inhibited MCF-7\/LCC2 and MCF-7\/LCC9 at 9.35 and 9.06 \u00b5M, respectively, within 24\u2009h of treatment. Moreover, IC<jats:sub>50<\/jats:sub> concentration treatment for 24\u2009h led to over a 50% reduction in cell motility and a 62% and 35% decrease in cell invasion in MCF-7\/LCC2 and MCF-7\/LCC9 cells, respectively. Metastasis biomarkers demonstrated that IVM treatment reduced the expression of vimentin and snail. The study also discovered that IVM diminished the expression of Wnt5a\/b and lipoprotein receptor-related protein 6 (LRP6), associated with the metastasis-related Wnt signaling pathway. In conclusion, IVM inhibits the Wnt signaling pathway associated with EMT in the metastasis of endocrine-resistant breast cancer cells. These insights underscore the potential of repurposing IVM for endocrine-resistant breast cancer patients.<\/jats:p>","DOI":"10.1371\/journal.pone.0326742","type":"journal-article","created":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T17:40:44Z","timestamp":1750959644000},"page":"e0326742","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["Ivermectin inhibits epithelial-to-mesenchymal transition via Wnt signaling in endocrine-resistant breast cancer 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