{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T08:48:44Z","timestamp":1787906924260,"version":"build-2784847793"},"reference-count":53,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T00:00:00Z","timestamp":1750636800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    <jats:italic>Aleuritopteris bicolor<\/jats:italic>\n                    (Roxb.) Fraser-Jenk. is used in traditional medicine in Nepal to treat a variety of ailments. This study aimed to investigate qualitative phytochemical screening, quantitative phytochemical analysis (total phenolic and flavonoid content estimation),\n                    <jats:italic>in vitro<\/jats:italic>\n                    antioxidant activity, alpha-amylase inhibitory activity of\n                    <jats:italic>Aleuritopteris bicolor<\/jats:italic>\n                    (Roxb.) Fraser-Jenk. leaves from Nepal. Furthermore, this study examined the analgesic (tail flick and hot plate test method) and anti-inflammatory (carrageenan-induced paw edema) activities of\n                    <jats:italic>Aleuritopteris bicolor<\/jats:italic>\n                    leaves in an acute pain and inflammation rat model. The\n                    <jats:italic>Aleuritopteris bicolor<\/jats:italic>\n                    extract exhibited flavonoid (405.95\u2009\u00b1\u20090.28\u2009mg QE\/g) and phenolic content (20.98\u2009\u00b1\u20090.20\u2009mg GAE\/g).\n                    <jats:italic>Aleuritopteris bicolor<\/jats:italic>\n                    showed free radical scavenging activity with IC\n                    <jats:sub>50<\/jats:sub>\n                    value in difference antioxidant methods (IC\n                    <jats:sub>50<\/jats:sub>\n                    :9.87 \u00b5g\/mL, DPPH scavenging assay; 249.59 \u00b5g\/mL, Hydrogen peroxide; 72.98 \u00b5g\/mL, Nitric oxide assay), and significant Ferric reducing power assay (0.51\u2009\u00b1\u20090.004). It increased analgesic activity by 38.89% (hot plate test) and 16.84% (tail-flick test) at 250\u2009mg\/kg, reduced inflammation by 36.44% at 180\u2009min (500\u2009mg\/kg), and inhibited alpha-amylase activity (IC\n                    <jats:sub>50<\/jats:sub>\n                    :59.31 \u00b5g\/mL).\n                    <jats:italic>Aleuritopteris bicolor<\/jats:italic>\n                    exhibits significant analgesic, anti-inflammatory, and hypoglycemic potential, making it a promising candidate for further pharmacological exploration. This study marks the first scientific study of the\n                    <jats:italic>in vivo<\/jats:italic>\n                    analgesic and anti-inflammatory effects from the leaf extract of\n                    <jats:italic>Aleuritopteris bicolor<\/jats:italic>\n                    till date.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0326808","type":"journal-article","created":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T13:40:07Z","timestamp":1750686007000},"page":"e0326808","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Anti-inflammatory, analgesic, antioxidant, and alpha-amylase inhibitory effects of the hydroethanolic leaf extract of Aleuritopteris bicolor (Roxb.) Fraser-Jenk."],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4782-5013","authenticated-orcid":true,"given":"Prabhat Kumar","family":"Jha","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1123-2806","authenticated-orcid":true,"given":"Bipindra","family":"Pandey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram Kishor","family":"Yadav","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. C.","family":"Sindhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandesh","family":"Poudel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sushil","family":"Panta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,6,23]]},"reference":[{"issue":"9","key":"pone.0326808.ref001","doi-asserted-by":"crossref","first-page":"4596","DOI":"10.3390\/ijms23094596","article-title":"Inflammation: a new look at an old problem","volume":"23","author":"E Gusev","year":"2022","journal-title":"Int J Mol Sci"},{"issue":"3","key":"pone.0326808.ref002","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1007\/s10238-022-00829-w","article-title":"Pathophysiology of deep vein thrombosis","volume":"23","author":"S Navarrete","year":"2023","journal-title":"Clin Exp 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