{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:39:48Z","timestamp":1783039188078,"version":"3.54.6"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T00:00:00Z","timestamp":1753228800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>The <jats:italic>bla<\/jats:italic><jats:sub>IMP<\/jats:sub> resistance gene encodes a metallo-beta-lactamase in bacteria, which confers reduced susceptibility or resistance to all the beta-lactams, including carbapenems which are critical for treating life-threatening infections. The dissemination of <jats:italic>bla<\/jats:italic><jats:sub>IMP<\/jats:sub> among various taxonomic families shows the diversity and range of horizontal gene transfer. Using short-read whole genome sequencing and bioinformatic tools, we determined the genetic motifs surrounding <jats:italic>bla<\/jats:italic><jats:sub>IMP<\/jats:sub> present in 32 bacterial isolates recovered from environmental sources and agriculture facilities. <jats:italic>bla<\/jats:italic><jats:sub>IMP<\/jats:sub> can be located extra-chromosomally on plasmids or within incomplete and complete Tn7 chromosomal structures. We identified a complete Tn7 transposon harboring the <jats:italic>bla<\/jats:italic><jats:sub>IMP-27<\/jats:sub> gene cassette within a class 2 integron located in chromosomal contigs of <jats:italic>Shewanella<\/jats:italic> spp<jats:italic>.<\/jats:italic> and <jats:italic>Providencia<\/jats:italic> spp. <jats:italic>Acinetobacter<\/jats:italic> spp. isolates were observed with truncated and incomplete Tn7 transposons, while conserving the class 2 integron and resistance gene cassettes. Additionally, IncQ1 plasmids carried by <jats:italic>Proteus<\/jats:italic> spp<jats:italic>., Escherichia coli,<\/jats:italic> and other <jats:italic>Enterobacteriaceae<\/jats:italic> spp<jats:italic>.<\/jats:italic> harbored class 2 integrons with <jats:italic>bla<\/jats:italic><jats:sub>IMP-64<\/jats:sub> and <jats:italic>sat<\/jats:italic>2 resistance gene cassettes. In an <jats:italic>Acidovorax<\/jats:italic> sp. isolate, <jats:italic>bla<\/jats:italic><jats:sub>IMP-27<\/jats:sub> and <jats:italic>sat<\/jats:italic>2 gene cassettes were found associated with an insertion sequence, ISL3 transposase, in an RP4 plasmid. The conserved structure of Tn7 in <jats:italic>Shewanella<\/jats:italic> spp. and <jats:italic>Providencia<\/jats:italic> spp. is consistent with these species being potential reservoirs from which other bacterial species have acquired partial Tn7 motifs, and the <jats:italic>bla<\/jats:italic><jats:sub>IMP-27<\/jats:sub> gene cassette. These data contribute to a broader understanding of the dissemination and temporality of <jats:italic>bla<\/jats:italic><jats:sub>IMP<\/jats:sub> alleles and their mobile genetic elements.<\/jats:p>","DOI":"10.1371\/journal.pone.0327200","type":"journal-article","created":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T17:24:47Z","timestamp":1753291487000},"page":"e0327200","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["The genetic context of blaIMP varies among bacterial families from One Health sources"],"prefix":"10.1371","volume":"20","author":[{"given":"Susan Vaughn","family":"Grooters","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dixie 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