{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:21:55Z","timestamp":1791228115881,"version":"4.1.0"},"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T00:00:00Z","timestamp":1753142400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>There is a strong need to develop technologies that reduce anthropogenic pollution and the dependence on nonrenewable Earth resources. One way of doing so is by harnessing biological systems for replacing the production of fossil-fuel based goods with low-environmental-impact alternatives. Recently, progress was made in engineering the model organism <jats:italic>E. coli<\/jats:italic> to grow using CO<jats:sub>2<\/jats:sub> and formate as its only carbon and energy sources using the reductive glycine pathway (rGlyP). Here, we use this engineered strain of <jats:italic>E. coli<\/jats:italic> as a host system for the production of polyhydroxybutyrate (PHB), a biologically derived and biodegradable plastic. The production of PHB in this strain was confirmed using Nile red fluorescence microscopy, transmission electron microscopy, and HPLC analysis, with a yield of 0.172\u2009\u00b1\u20090.005\u2009mg\/L of PHB after 120 hours of incubation. Since formate can be efficiently generated from CO<jats:sub>2<\/jats:sub> by electrochemical reduction using renewable energy sources, this study serves as a proof of concept for the emerging field of electro-bioproduction.<\/jats:p>","DOI":"10.1371\/journal.pone.0327512","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T17:23:18Z","timestamp":1753204998000},"page":"e0327512","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Demonstration of bioplastic production from CO2 and formate using the reductive glycine pathway in E. coli"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4160-5607","authenticated-orcid":true,"given":"Daria","family":"Fedorova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roee","family":"Ben-Nissan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliya","family":"Milshtein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cassandra","family":"Reyes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghil","family":"Jona","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nili","family":"Dezorella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1167-0849","authenticated-orcid":true,"given":"Gil","family":"Feiguelman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5341-3066","authenticated-orcid":true,"given":"Rostislav","family":"Fedorov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aya","family":"Gomaa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariel B.","family":"Lindner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8776-4799","authenticated-orcid":true,"given":"Elad","family":"Noor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1641-2299","authenticated-orcid":true,"given":"Ron","family":"Milo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,7,22]]},"reference":[{"key":"pone.0327512.ref001","doi-asserted-by":"crossref","DOI":"10.1787\/9789264307414-en","volume-title":"The future of petrochemicals: Towards more sustainable plastics and fertilisers","author":"International Energy Agency","year":"2018"},{"key":"pone.0327512.ref002","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/j.rser.2016.12.076","article-title":"Bioethanol production from renewable sources: Current perspectives and technological progress","volume":"71","author":"H Zabed","year":"2017","journal-title":"Renewable and Sustainable Energy Reviews"},{"issue":"1","key":"pone.0327512.ref003","doi-asserted-by":"crossref","first-page":"22521","DOI":"10.1038\/srep22521","article-title":"The water-land-food nexus of first-generation biofuels","volume":"6","author":"MC Rulli","year":"2016","journal-title":"Scientific Reports"},{"issue":"1","key":"pone.0327512.ref004","doi-asserted-by":"crossref","first-page":"2844","DOI":"10.1038\/s41467-019-10775-z","article-title":"Global impacts of future cropland expansion and intensification on agricultural markets and biodiversity","volume":"10","author":"F Zabel","year":"2019","journal-title":"Nat Commun"},{"issue":"5734","key":"pone.0327512.ref005","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1126\/science.1111772","article-title":"Global consequences of land use","volume":"309","author":"JA Foley","year":"2005","journal-title":"Science"},{"issue":"24","key":"pone.0327512.ref006","doi-asserted-by":"crossref","first-page":"7402","DOI":"10.1073\/pnas.1406485112","article-title":"Spatial patterns of agricultural expansion determine impacts on biodiversity and carbon storage","volume":"112","author":"R Chaplin-Kramer","year":"2015","journal-title":"Proc Natl Acad Sci U S A"},{"key":"pone.0327512.ref007","doi-asserted-by":"crossref","first-page":"171296","DOI":"10.1016\/j.scitotenv.2024.171296","article-title":"Biodiversity responses to agricultural practices in cropland and natural habitats","volume":"922","author":"J Zhao","year":"2024","journal-title":"Sci Total Environ"},{"issue":"26","key":"pone.0327512.ref008","doi-asserted-by":"crossref","first-page":"269","DOI":"10.17230\/ingciencia.13.26.10","article-title":"From obtaining to degradation of PHB: Material properties. 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